Adaptor polymerase chain reaction for single molecule amplification

Michihiko Nakano; Jun Komatsu; Hirofumi Kurita; Hachiro Yasuda; Shinji Katsura; Akira Mizuno

doi:10.1263/jbb.100.216

Adaptor polymerase chain reaction for single molecule amplification

Michihiko Nakano, Jun Komatsu, Hirofumi Kurita, Hachiro Yasuda, Shinji Katsura, Akira Mizuno

Research output: Contribution to journal › Article

2 Citations (Scopus)

Abstract

The adaptor polymerase chain reaction (PCR) permits the amplification of DNA fragments with arbitrary sequences. In this paper, we describe the successful amplification of plasmid-derived single molecule DNAs digested by a restriction enzyme. By using adaptors made of short and long oligonucleotides, nonspecific interactions during PCR were suppressed. The method will be applicable to the detection of single molecule DNA fragments even if their sequence is unknown.

Original language	English
Pages (from-to)	216-218
Number of pages	3
Journal	Journal of Bioscience and Bioengineering
Volume	100
Issue number	2
DOIs	https://doi.org/10.1263/jbb.100.216
Publication status	Published - Jan 1 2005
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1263/jbb.100.216

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Nakano, M., Komatsu, J., Kurita, H., Yasuda, H., Katsura, S., & Mizuno, A. (2005). Adaptor polymerase chain reaction for single molecule amplification. Journal of Bioscience and Bioengineering, 100(2), 216-218. https://doi.org/10.1263/jbb.100.216

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AU - Nakano, Michihiko

AU - Komatsu, Jun

AU - Kurita, Hirofumi

AU - Yasuda, Hachiro

AU - Katsura, Shinji

AU - Mizuno, Akira

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