Adaptor polymerase chain reaction for single molecule amplification

Michihiko Nakano, Jun Komatsu, Hirofumi Kurita, Hachiro Yasuda, Shinji Katsura, Akira Mizuno

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The adaptor polymerase chain reaction (PCR) permits the amplification of DNA fragments with arbitrary sequences. In this paper, we describe the successful amplification of plasmid-derived single molecule DNAs digested by a restriction enzyme. By using adaptors made of short and long oligonucleotides, nonspecific interactions during PCR were suppressed. The method will be applicable to the detection of single molecule DNA fragments even if their sequence is unknown.

Original languageEnglish
Pages (from-to)216-218
Number of pages3
JournalJournal of Bioscience and Bioengineering
Volume100
Issue number2
DOIs
Publication statusPublished - Jan 1 2005
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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