Abstract
The adaptor polymerase chain reaction (PCR) permits the amplification of DNA fragments with arbitrary sequences. In this paper, we describe the successful amplification of plasmid-derived single molecule DNAs digested by a restriction enzyme. By using adaptors made of short and long oligonucleotides, nonspecific interactions during PCR were suppressed. The method will be applicable to the detection of single molecule DNA fragments even if their sequence is unknown.
| Original language | English |
|---|---|
| Pages (from-to) | 216-218 |
| Number of pages | 3 |
| Journal | Journal of Bioscience and Bioengineering |
| Volume | 100 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jan 1 2005 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
Cite this
Adaptor polymerase chain reaction for single molecule amplification. / Nakano, Michihiko; Komatsu, Jun; Kurita, Hirofumi; Yasuda, Hachiro; Katsura, Shinji; Mizuno, Akira.
In: Journal of Bioscience and Bioengineering, Vol. 100, No. 2, 01.01.2005, p. 216-218.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Adaptor polymerase chain reaction for single molecule amplification
AU - Nakano, Michihiko
AU - Komatsu, Jun
AU - Kurita, Hirofumi
AU - Yasuda, Hachiro
AU - Katsura, Shinji
AU - Mizuno, Akira
PY - 2005/1/1
Y1 - 2005/1/1
N2 - The adaptor polymerase chain reaction (PCR) permits the amplification of DNA fragments with arbitrary sequences. In this paper, we describe the successful amplification of plasmid-derived single molecule DNAs digested by a restriction enzyme. By using adaptors made of short and long oligonucleotides, nonspecific interactions during PCR were suppressed. The method will be applicable to the detection of single molecule DNA fragments even if their sequence is unknown.
AB - The adaptor polymerase chain reaction (PCR) permits the amplification of DNA fragments with arbitrary sequences. In this paper, we describe the successful amplification of plasmid-derived single molecule DNAs digested by a restriction enzyme. By using adaptors made of short and long oligonucleotides, nonspecific interactions during PCR were suppressed. The method will be applicable to the detection of single molecule DNA fragments even if their sequence is unknown.
UR - http://www.scopus.com/inward/record.url?scp=27644576868&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=27644576868&partnerID=8YFLogxK
U2 - 10.1263/jbb.100.216
DO - 10.1263/jbb.100.216
M3 - Article
C2 - 16198268
AN - SCOPUS:27644576868
VL - 100
SP - 216
EP - 218
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
SN - 1389-1723
IS - 2
ER -