Adaptor polymerase chain reaction for single molecule amplification

Michihiko Nakano, Jun Komatsu, Hirofumi Kurita, Hachiro Yasuda, Shinji Katsura, Akira Mizuno

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The adaptor polymerase chain reaction (PCR) permits the amplification of DNA fragments with arbitrary sequences. In this paper, we describe the successful amplification of plasmid-derived single molecule DNAs digested by a restriction enzyme. By using adaptors made of short and long oligonucleotides, nonspecific interactions during PCR were suppressed. The method will be applicable to the detection of single molecule DNA fragments even if their sequence is unknown.

Original languageEnglish
Pages (from-to)216-218
Number of pages3
JournalJournal of Bioscience and Bioengineering
Volume100
Issue number2
DOIs
Publication statusPublished - Jan 1 2005
Externally publishedYes

Fingerprint

Polymerase chain reaction
Amplification
DNA
Polymerase Chain Reaction
Molecules
Oligonucleotides
Plasmids
Enzymes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Adaptor polymerase chain reaction for single molecule amplification. / Nakano, Michihiko; Komatsu, Jun; Kurita, Hirofumi; Yasuda, Hachiro; Katsura, Shinji; Mizuno, Akira.

In: Journal of Bioscience and Bioengineering, Vol. 100, No. 2, 01.01.2005, p. 216-218.

Research output: Contribution to journalArticle

Nakano, M, Komatsu, J, Kurita, H, Yasuda, H, Katsura, S & Mizuno, A 2005, 'Adaptor polymerase chain reaction for single molecule amplification', Journal of Bioscience and Bioengineering, vol. 100, no. 2, pp. 216-218. https://doi.org/10.1263/jbb.100.216
Nakano, Michihiko ; Komatsu, Jun ; Kurita, Hirofumi ; Yasuda, Hachiro ; Katsura, Shinji ; Mizuno, Akira. / Adaptor polymerase chain reaction for single molecule amplification. In: Journal of Bioscience and Bioengineering. 2005 ; Vol. 100, No. 2. pp. 216-218.
@article{3fd5820b2e324cc59790b60c663bef7b,
title = "Adaptor polymerase chain reaction for single molecule amplification",
abstract = "The adaptor polymerase chain reaction (PCR) permits the amplification of DNA fragments with arbitrary sequences. In this paper, we describe the successful amplification of plasmid-derived single molecule DNAs digested by a restriction enzyme. By using adaptors made of short and long oligonucleotides, nonspecific interactions during PCR were suppressed. The method will be applicable to the detection of single molecule DNA fragments even if their sequence is unknown.",
author = "Michihiko Nakano and Jun Komatsu and Hirofumi Kurita and Hachiro Yasuda and Shinji Katsura and Akira Mizuno",
year = "2005",
month = "1",
day = "1",
doi = "10.1263/jbb.100.216",
language = "English",
volume = "100",
pages = "216--218",
journal = "Journal of Bioscience and Bioengineering",
issn = "1389-1723",
publisher = "The Society for Biotechnology, Japan",
number = "2",

}

TY - JOUR

T1 - Adaptor polymerase chain reaction for single molecule amplification

AU - Nakano, Michihiko

AU - Komatsu, Jun

AU - Kurita, Hirofumi

AU - Yasuda, Hachiro

AU - Katsura, Shinji

AU - Mizuno, Akira

PY - 2005/1/1

Y1 - 2005/1/1

N2 - The adaptor polymerase chain reaction (PCR) permits the amplification of DNA fragments with arbitrary sequences. In this paper, we describe the successful amplification of plasmid-derived single molecule DNAs digested by a restriction enzyme. By using adaptors made of short and long oligonucleotides, nonspecific interactions during PCR were suppressed. The method will be applicable to the detection of single molecule DNA fragments even if their sequence is unknown.

AB - The adaptor polymerase chain reaction (PCR) permits the amplification of DNA fragments with arbitrary sequences. In this paper, we describe the successful amplification of plasmid-derived single molecule DNAs digested by a restriction enzyme. By using adaptors made of short and long oligonucleotides, nonspecific interactions during PCR were suppressed. The method will be applicable to the detection of single molecule DNA fragments even if their sequence is unknown.

UR - http://www.scopus.com/inward/record.url?scp=27644576868&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=27644576868&partnerID=8YFLogxK

U2 - 10.1263/jbb.100.216

DO - 10.1263/jbb.100.216

M3 - Article

C2 - 16198268

AN - SCOPUS:27644576868

VL - 100

SP - 216

EP - 218

JO - Journal of Bioscience and Bioengineering

JF - Journal of Bioscience and Bioengineering

SN - 1389-1723

IS - 2

ER -