@article{3fd5820b2e324cc59790b60c663bef7b,
title = "Adaptor polymerase chain reaction for single molecule amplification",
abstract = "The adaptor polymerase chain reaction (PCR) permits the amplification of DNA fragments with arbitrary sequences. In this paper, we describe the successful amplification of plasmid-derived single molecule DNAs digested by a restriction enzyme. By using adaptors made of short and long oligonucleotides, nonspecific interactions during PCR were suppressed. The method will be applicable to the detection of single molecule DNA fragments even if their sequence is unknown.",
author = "Michihiko Nakano and Jun Komatsu and Hirofumi Kurita and Hachiro Yasuda and Shinji Katsura and Akira Mizuno",
note = "Funding Information: The authors are grateful to Assoc. Prof. Hideo Nakano and Prof. Tsuneo Yamane of Nagoya University for valuable discussions and providing primer sequence information. This work was supported in part by a Grant-in-Aid for Scientific Research of the Ministry of Education, Culture, Sports, Science and Technology, Japan (no. 15360440), the Japan Society for the Promotion of Science (JSPS) Research Fellowships for Young Scientists (no. 08351), and also by the 21st Century COE Program “Ecological Engineering for Homeostatic Human Activities”, from the Ministry of Education, Culture, Sports, Science and Technology.",
year = "2005",
doi = "10.1263/jbb.100.216",
language = "English",
volume = "100",
pages = "216--218",
journal = "Journal of Bioscience and Bioengineering",
issn = "1389-1723",
publisher = "The Society for Biotechnology, Japan",
number = "2",
}