Additive roles of XPA and MSH2 genes in UVB-induced skin tumorigenesis in mice

Masafumi Yoshino, Yoshimichi Nakatsu, Hein te Riele, Seiichi Hirota, Yukihiko Kitamura, Kiyoji Tanaka

Research output: Contribution to journalArticle

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Abstract

We have made xeroderma pigmentosum group A gene (XPA)-knockout mice (XPA-/- mice). The XPA-/- mice had no detectable activity for nucleotide excision repair (NER) and showed a high incidence of UVB-induced skin tumorigenesis. We have also found that cell lines derived from skin cancers in UVB-irradiated XPA-/- mice become tolerant to UV-irradiation and showed abnormal UV-induced cell cycle checkpoints and decreased mismatch repair (MMR) activity. These results suggested that the MMR-downregulation may help cells escape killing by UV-irradiation and thus MMR-deficient clones are selected for during the tumorigenic transformation of XPA-/- cells. In this report, we examined whether the incidence of UVB-induced skin tumorigenesis is enhanced in XPA-/-MSH2-/-, XPA-/- and MSH2-/- mice when compared with that in wild-type mice. Our results indicate that the MSH2-deficiency caused a high incidence of spontaneous and UVB-induced skin tumorigenesis and the XPA and MSH2 genes have additive roles in the UV-induced skin tumorigenesis.

Original languageEnglish
Pages (from-to)935-940
Number of pages6
JournalDNA Repair
Volume1
Issue number11
DOIs
Publication statusPublished - Nov 1 2002
Externally publishedYes

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DNA Mismatch Repair
Skin
Carcinogenesis
Genes
Knockout Mice
Repair
Incidence
Neoplastic Cell Transformation
Xeroderma Pigmentosum
Gene Knockout Techniques
Cells
Irradiation
Skin Neoplasms
Cell Cycle Checkpoints
DNA Repair
Down-Regulation
Clone Cells
Cell Line
Nucleotides

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Additive roles of XPA and MSH2 genes in UVB-induced skin tumorigenesis in mice. / Yoshino, Masafumi; Nakatsu, Yoshimichi; Riele, Hein te; Hirota, Seiichi; Kitamura, Yukihiko; Tanaka, Kiyoji.

In: DNA Repair, Vol. 1, No. 11, 01.11.2002, p. 935-940.

Research output: Contribution to journalArticle

Yoshino, M, Nakatsu, Y, Riele, HT, Hirota, S, Kitamura, Y & Tanaka, K 2002, 'Additive roles of XPA and MSH2 genes in UVB-induced skin tumorigenesis in mice', DNA Repair, vol. 1, no. 11, pp. 935-940. https://doi.org/10.1016/S1568-7864(02)00144-1
Yoshino, Masafumi ; Nakatsu, Yoshimichi ; Riele, Hein te ; Hirota, Seiichi ; Kitamura, Yukihiko ; Tanaka, Kiyoji. / Additive roles of XPA and MSH2 genes in UVB-induced skin tumorigenesis in mice. In: DNA Repair. 2002 ; Vol. 1, No. 11. pp. 935-940.
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