Adenovirus-mediated ectopic expression of Msx2 in even-numbered rhombomeres induces apoptotic elimination of cranial neural crest cells in ovo

Katsu Takahashi, Glen H. Nuckolls, Osamu Tanaka, Ichiro Semba, Ichiro Takahashi, Ralph Dashner, Lillian Shum, Harold C. Slavkin

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50 Citations (Scopus)

Abstract

Distinct cranial neural crest-derived cell types (a number of neuronal as well as non-neuronal cell lineages) are generated at characteristic times and positions in the rhombomeres of the hindbrain in developing vertebrate embryos. To examine this developmental process, we developed a novel strategy designed to test the efficacy of gain-of-function Msx2 expression within rhombomeres in ovo prior to the emigration of cranial neural crest cells (CNCC). Previous studies indicate that CNCC from odd-numbered rhombomeres (r3 and r5) undergo apoptosis in response to exogenous BMP4. We provide evidence that targeted infection in ovo using adenovirus containing Msx2 and a reporter molecule indicative of translation can induce apoptosis in either even- or odd-numbered rhombomeres. Furthermore, infected lacZ-control explants indicated that CNCC emigrated, and that 20% of these cells were double positive for crest cell markers HNK-1 and β-gal. In contrast, there were no HNK-1 and Msx2 double positive cells emigrating from Msx2 infected explants. These results support the hypothesis that apoptotic elimination of CNCC can be induced by 'gain-of-function' Msx2 expression in even-numbered rhombomeres. These inductive interactions involve qualitative, quantitative, positional and temporal differences in TGF-β-related signals, Msx2 expression and other transcriptional control.

Original languageEnglish
Pages (from-to)1627-1635
Number of pages9
JournalDevelopment
Volume125
Issue number9
Publication statusPublished - 1998
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Cell Biology

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    Takahashi, K., Nuckolls, G. H., Tanaka, O., Semba, I., Takahashi, I., Dashner, R., Shum, L., & Slavkin, H. C. (1998). Adenovirus-mediated ectopic expression of Msx2 in even-numbered rhombomeres induces apoptotic elimination of cranial neural crest cells in ovo. Development, 125(9), 1627-1635.