Abstract
The nucleus tractus solitarii (NTS) is an important site for the regulation of sympathetic nerve activity. It receives the signals through afferent fibers from arterial baroreceptors, chemoreceptors, cardiopulmonary receptors, and other visceral receptors. Many studies have examined the role of nitric oxide (NO) in the NTS in cardiovascular regulation. However, most of these studies were conducted in an acute state with anesthesia. We have developed a novel technique of endothelial nitric oxide synthase (eNOS) gene transfer into the NTS in vivo. Adenovirus vectors encoding either the β-galactosidase gene (Adβgal) or the endothelial nitric oxide synthase gene (Ad-eNOS) gene were transfected into the NTS. In the Adβgal-treated rats, the local expression of β-galactosidase was confirmed by X-Gal staining, and β-galactosidase activity was quantified using a colorimetric assay. In the AdeNOS-treated rats, the local expression of eNOS protein was confirmed by immunohistochemistry, and eNOS production was measured by in vivo microdialysis. Blood pressure and heart rate were monitored by a radiotelemetry system in a conscious state. The expression of each gene was observed from day 5 to day 10 after the gene transfer. In the AdeNOS-treated rats, blood pressure and heart rate significantly decreased from day 5 to day 10, and then thereafter gradually recovered over time. Our method may be useful in examining the local effect of a particular substance produced by a specific gene in the brain on cardiovascular function.
| Original language | English |
|---|---|
| Pages (from-to) | 197-205 |
| Number of pages | 9 |
| Journal | Annals of the New York Academy of Sciences |
| Volume | 940 |
| Publication status | Published - Jan 1 2001 |
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All Science Journal Classification (ASJC) codes
- Neuroscience(all)
- Biochemistry, Genetics and Molecular Biology(all)
- History and Philosophy of Science
Cite this
Adenovirus-mediated gene transfer into the NTS in conscious rats : A new approach to examining the central control of cardiovascular regulation. / Hirooka, Yoshitaka; Sakai, Koji; Kishi, Takuya; Takeshita, Akira.
In: Annals of the New York Academy of Sciences, Vol. 940, 01.01.2001, p. 197-205.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Adenovirus-mediated gene transfer into the NTS in conscious rats
T2 - A new approach to examining the central control of cardiovascular regulation
AU - Hirooka, Yoshitaka
AU - Sakai, Koji
AU - Kishi, Takuya
AU - Takeshita, Akira
PY - 2001/1/1
Y1 - 2001/1/1
N2 - The nucleus tractus solitarii (NTS) is an important site for the regulation of sympathetic nerve activity. It receives the signals through afferent fibers from arterial baroreceptors, chemoreceptors, cardiopulmonary receptors, and other visceral receptors. Many studies have examined the role of nitric oxide (NO) in the NTS in cardiovascular regulation. However, most of these studies were conducted in an acute state with anesthesia. We have developed a novel technique of endothelial nitric oxide synthase (eNOS) gene transfer into the NTS in vivo. Adenovirus vectors encoding either the β-galactosidase gene (Adβgal) or the endothelial nitric oxide synthase gene (Ad-eNOS) gene were transfected into the NTS. In the Adβgal-treated rats, the local expression of β-galactosidase was confirmed by X-Gal staining, and β-galactosidase activity was quantified using a colorimetric assay. In the AdeNOS-treated rats, the local expression of eNOS protein was confirmed by immunohistochemistry, and eNOS production was measured by in vivo microdialysis. Blood pressure and heart rate were monitored by a radiotelemetry system in a conscious state. The expression of each gene was observed from day 5 to day 10 after the gene transfer. In the AdeNOS-treated rats, blood pressure and heart rate significantly decreased from day 5 to day 10, and then thereafter gradually recovered over time. Our method may be useful in examining the local effect of a particular substance produced by a specific gene in the brain on cardiovascular function.
AB - The nucleus tractus solitarii (NTS) is an important site for the regulation of sympathetic nerve activity. It receives the signals through afferent fibers from arterial baroreceptors, chemoreceptors, cardiopulmonary receptors, and other visceral receptors. Many studies have examined the role of nitric oxide (NO) in the NTS in cardiovascular regulation. However, most of these studies were conducted in an acute state with anesthesia. We have developed a novel technique of endothelial nitric oxide synthase (eNOS) gene transfer into the NTS in vivo. Adenovirus vectors encoding either the β-galactosidase gene (Adβgal) or the endothelial nitric oxide synthase gene (Ad-eNOS) gene were transfected into the NTS. In the Adβgal-treated rats, the local expression of β-galactosidase was confirmed by X-Gal staining, and β-galactosidase activity was quantified using a colorimetric assay. In the AdeNOS-treated rats, the local expression of eNOS protein was confirmed by immunohistochemistry, and eNOS production was measured by in vivo microdialysis. Blood pressure and heart rate were monitored by a radiotelemetry system in a conscious state. The expression of each gene was observed from day 5 to day 10 after the gene transfer. In the AdeNOS-treated rats, blood pressure and heart rate significantly decreased from day 5 to day 10, and then thereafter gradually recovered over time. Our method may be useful in examining the local effect of a particular substance produced by a specific gene in the brain on cardiovascular function.
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UR - http://www.scopus.com/inward/citedby.url?scp=0034931189&partnerID=8YFLogxK
M3 - Article
C2 - 11458677
AN - SCOPUS:0034931189
VL - 940
SP - 197
EP - 205
JO - Annals of the New York Academy of Sciences
JF - Annals of the New York Academy of Sciences
SN - 0077-8923
ER -