Adenovirus-mediated gene transfer into the NTS in conscious rats: A new approach to examining the central control of cardiovascular regulation

Yoshitaka Hirooka, Koji Sakai, Takuya Kishi, Akira Takeshita

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The nucleus tractus solitarii (NTS) is an important site for the regulation of sympathetic nerve activity. It receives the signals through afferent fibers from arterial baroreceptors, chemoreceptors, cardiopulmonary receptors, and other visceral receptors. Many studies have examined the role of nitric oxide (NO) in the NTS in cardiovascular regulation. However, most of these studies were conducted in an acute state with anesthesia. We have developed a novel technique of endothelial nitric oxide synthase (eNOS) gene transfer into the NTS in vivo. Adenovirus vectors encoding either the β-galactosidase gene (Adβgal) or the endothelial nitric oxide synthase gene (Ad-eNOS) gene were transfected into the NTS. In the Adβgal-treated rats, the local expression of β-galactosidase was confirmed by X-Gal staining, and β-galactosidase activity was quantified using a colorimetric assay. In the AdeNOS-treated rats, the local expression of eNOS protein was confirmed by immunohistochemistry, and eNOS production was measured by in vivo microdialysis. Blood pressure and heart rate were monitored by a radiotelemetry system in a conscious state. The expression of each gene was observed from day 5 to day 10 after the gene transfer. In the AdeNOS-treated rats, blood pressure and heart rate significantly decreased from day 5 to day 10, and then thereafter gradually recovered over time. Our method may be useful in examining the local effect of a particular substance produced by a specific gene in the brain on cardiovascular function.

Original languageEnglish
Pages (from-to)197-205
Number of pages9
JournalAnnals of the New York Academy of Sciences
Volume940
Publication statusPublished - Jan 1 2001

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Gene transfer
Solitary Nucleus
Adenoviridae
Nitric Oxide Synthase Type III
Galactosidases
Rats
Genes
Blood pressure
Heart Rate
Blood Pressure
Pressoreceptors
Microdialysis
Assays
Brain
Nitric Oxide
Nucleus
Conscious
Gene
Rat
Anesthesia

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

Cite this

Adenovirus-mediated gene transfer into the NTS in conscious rats : A new approach to examining the central control of cardiovascular regulation. / Hirooka, Yoshitaka; Sakai, Koji; Kishi, Takuya; Takeshita, Akira.

In: Annals of the New York Academy of Sciences, Vol. 940, 01.01.2001, p. 197-205.

Research output: Contribution to journalArticle

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