Adhesion and signal transduction of endothelial cells on a honeycomb films pre-coated by fibronectin

Sadaaki Yamamot, Masaru Tanaka, Hiroshi Sunami, Yuka Mortta, Emio Ito, Keiko Arai, Shigeko Yamashita, Masatsugu Shimomura

Research output: Contribution to conferencePaperpeer-review

Abstract

In order to determine how cells respond to a honeycomb film of poly(ε-caprolactone) (PCL), focal adhesion of porcine aortic endothelial cells (PAECs) cultured on the Fn coated honeycomb films in a serum free medium were characterized by using immunofluorescencet labeling of vinculin and focal adhesion kinase autophosphorylated at the tyrosine residue 397 (pY397FAK).. The sites of pY397FAK and vinculin were overlapped and agreed well with the adsorption site of Fn fibrils. This demonstrated that PAECs adhered onto the honeycomb films at focal contact points localized around pore periphery. The expression of pY397FAK was 3 times higher than that on a PCL flat film as a reference. These results imply that the signaling mediated by a integrin receptor - Fn binding were activated more effectively on honeycomb films compared with on a flat film The cell response to honeycomb films (adhesion pattern and phosphorilation of FAK) was supposed to be originated from the regularly arraigned adsorption pattern of Fn determined by the pore structure of the film.

Original languageEnglish
Pages5493-5494
Number of pages2
Publication statusPublished - Dec 1 2006
Externally publishedYes
Event55th Society of Polymer Science Japan Symposium on Macromolecules - Toyama, Japan
Duration: Sep 20 2006Sep 22 2006

Other

Other55th Society of Polymer Science Japan Symposium on Macromolecules
Country/TerritoryJapan
CityToyama
Period9/20/069/22/06

All Science Journal Classification (ASJC) codes

  • Engineering(all)

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