Adhesion of Escherichia coli onto oxide minerals - extended dlvo theory and Flotation behaviour

M. Farahat, T. Hirajima, Keiko Sasaki, Katsumi Doi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The adhesion behaviour of Escherichia coli to three oxide minerals (quartz, hematite and corundum) was investigated experimentally. A strain of E. coli was used that contains genes encoding a protein involved in silica precipitation. Maximum cell adhesion was observed at pH <4.3 for quartz and at pH 4.5 - 8.5 for corundum. Cell adhesion to hematite remained low at all pHs. Microbe-mineral adhesion was assessed with an extended DLVO theory approach. The essential parameters used to calculate the energy of the microbe-mineral interaction (Hamaker constants and acid-base components) were experimentally determined. The extended DLVO approach can be used to explain the results of the adhesion experiments. The effect of E coli on the floatability of the three oxide minerals was determined. Our results showed that Ecoli can act as a selective collector for quartz at acidic pHs, with 90 per cent of the quartz floated at 1.5 × 109 cells/mL. However, only 9 per cent of hematite and 30 per cent of corundum was floated under similar conditions. Using E coli and no reagents, it was possible to separate quartz from a hematite-quartz mixture with a Newton's efficiency of 0.7. Quartz was removed from a corundum mixture by E coli with a Newton's efficiency of 0.6.

Original languageEnglish
Title of host publicationXXV International Mineral Processing Congress 2010, IMPC 2010
Pages1841-1851
Number of pages11
Volume3
Publication statusPublished - 2010
Event25th International Mineral Processing Congress 2010, IMPC 2010 - Brisbane, QLD, Australia
Duration: Sep 6 2010Sep 10 2010

Other

Other25th International Mineral Processing Congress 2010, IMPC 2010
CountryAustralia
CityBrisbane, QLD
Period9/6/109/10/10

Fingerprint

Oxide minerals
Flotation
adhesion
Escherichia coli
Quartz
Adhesion
oxide
quartz
Corundum
corundum
Hematite
hematite
mineral
Cell adhesion
Minerals
Gene encoding
flotation
silica
Silica
Proteins

All Science Journal Classification (ASJC) codes

  • Earth-Surface Processes
  • Geology
  • Electrical and Electronic Engineering
  • Mechanical Engineering

Cite this

Farahat, M., Hirajima, T., Sasaki, K., & Doi, K. (2010). Adhesion of Escherichia coli onto oxide minerals - extended dlvo theory and Flotation behaviour. In XXV International Mineral Processing Congress 2010, IMPC 2010 (Vol. 3, pp. 1841-1851)

Adhesion of Escherichia coli onto oxide minerals - extended dlvo theory and Flotation behaviour. / Farahat, M.; Hirajima, T.; Sasaki, Keiko; Doi, Katsumi.

XXV International Mineral Processing Congress 2010, IMPC 2010. Vol. 3 2010. p. 1841-1851.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Farahat, M, Hirajima, T, Sasaki, K & Doi, K 2010, Adhesion of Escherichia coli onto oxide minerals - extended dlvo theory and Flotation behaviour. in XXV International Mineral Processing Congress 2010, IMPC 2010. vol. 3, pp. 1841-1851, 25th International Mineral Processing Congress 2010, IMPC 2010, Brisbane, QLD, Australia, 9/6/10.
Farahat M, Hirajima T, Sasaki K, Doi K. Adhesion of Escherichia coli onto oxide minerals - extended dlvo theory and Flotation behaviour. In XXV International Mineral Processing Congress 2010, IMPC 2010. Vol. 3. 2010. p. 1841-1851
Farahat, M. ; Hirajima, T. ; Sasaki, Keiko ; Doi, Katsumi. / Adhesion of Escherichia coli onto oxide minerals - extended dlvo theory and Flotation behaviour. XXV International Mineral Processing Congress 2010, IMPC 2010. Vol. 3 2010. pp. 1841-1851
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