Adhesion of Escherichia coli onto oxide minerals - extended dlvo theory and Flotation behaviour

M. Farahat, T. Hirajima, K. Sasaki, K. Doi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The adhesion behaviour of Escherichia coli to three oxide minerals (quartz, hematite and corundum) was investigated experimentally. A strain of E. coli was used that contains genes encoding a protein involved in silica precipitation. Maximum cell adhesion was observed at pH <4.3 for quartz and at pH 4.5 - 8.5 for corundum. Cell adhesion to hematite remained low at all pHs. Microbe-mineral adhesion was assessed with an extended DLVO theory approach. The essential parameters used to calculate the energy of the microbe-mineral interaction (Hamaker constants and acid-base components) were experimentally determined. The extended DLVO approach can be used to explain the results of the adhesion experiments. The effect of E coli on the floatability of the three oxide minerals was determined. Our results showed that Ecoli can act as a selective collector for quartz at acidic pHs, with 90 per cent of the quartz floated at 1.5 × 109 cells/mL. However, only 9 per cent of hematite and 30 per cent of corundum was floated under similar conditions. Using E coli and no reagents, it was possible to separate quartz from a hematite-quartz mixture with a Newton's efficiency of 0.7. Quartz was removed from a corundum mixture by E coli with a Newton's efficiency of 0.6.

Original languageEnglish
Title of host publicationXXV International Mineral Processing Congress 2010, IMPC 2010
Pages1841-1851
Number of pages11
Publication statusPublished - Dec 1 2010
Event25th International Mineral Processing Congress 2010, IMPC 2010 - Brisbane, QLD, Australia
Duration: Sep 6 2010Sep 10 2010

Publication series

NameXXV International Mineral Processing Congress 2010, IMPC 2010
Volume3

Other

Other25th International Mineral Processing Congress 2010, IMPC 2010
CountryAustralia
CityBrisbane, QLD
Period9/6/109/10/10

All Science Journal Classification (ASJC) codes

  • Earth-Surface Processes
  • Geology
  • Electrical and Electronic Engineering
  • Mechanical Engineering

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    Farahat, M., Hirajima, T., Sasaki, K., & Doi, K. (2010). Adhesion of Escherichia coli onto oxide minerals - extended dlvo theory and Flotation behaviour. In XXV International Mineral Processing Congress 2010, IMPC 2010 (pp. 1841-1851). (XXV International Mineral Processing Congress 2010, IMPC 2010; Vol. 3).