TY - JOUR
T1 - Adipogenesis of perfluorooctanesulfonate (pfos) on japanese medaka (oryzias latipes) embryo using ovo-nanoinjection-mRNA seq analysis
AU - Chen, Kun
AU - Iwasaki, Naoto
AU - Qiu, Xuchun
AU - Xu, Hai
AU - Takai, Yuki
AU - Tashiro, Kosuke
AU - Shimasaki, Yohei
AU - Oshima, Yuji
N1 - Funding Information:
This study was partly funded by the cooperative research program of the Institute of Nature and Environmental Technology, Kanazawa University Accept No. 5 in 2015.
PY - 2020
Y1 - 2020
N2 - Perfluorooctanesulfonate (PFOS) is a worldwide persistent organic pollutant and suspected to have effects on animals. The present study used in ovo-nanoinjection to simulate the maternal bioaccumulation and biotransformation of PFOS from parental Japanese medaka (Oryzias latipes) to embryos, investigated and elucidated the obesogenic activity in vivo and the gene expression changes. Results showed that no significant differences on the survival of medaka embryos and larvae between control, solvent control, and three PFOS exposure groups (0.05, 0.5 and 5 ng per embryo). Hatching time of medaka embryo was significantly delayed in the 0.05 ng PFOS exposure group and significantly accelerated in the 5 ng exposure group compare with those in control. The adipocyte areas were significantly increased in 0.05 and 0.5 ng per embryo PFOS exposure groups comparing to that of the control. The mRNA sequencing analysis on the medaka larvae chromosome gene expressions showed that 24 genes had significantly changed expressions (p < 0.05, FDR < 0.05) including genes involved in the pathways of metabolism and adipogenesis, like g6pca.2 in the 'Insulin signaling pathway' and 'Adipocytokine signaling pathway'. These results suggested that ovo-nanoinjection-mRNA Seq analysis is a reliable method to evaluate and understand the maternal effect of chemicals.
AB - Perfluorooctanesulfonate (PFOS) is a worldwide persistent organic pollutant and suspected to have effects on animals. The present study used in ovo-nanoinjection to simulate the maternal bioaccumulation and biotransformation of PFOS from parental Japanese medaka (Oryzias latipes) to embryos, investigated and elucidated the obesogenic activity in vivo and the gene expression changes. Results showed that no significant differences on the survival of medaka embryos and larvae between control, solvent control, and three PFOS exposure groups (0.05, 0.5 and 5 ng per embryo). Hatching time of medaka embryo was significantly delayed in the 0.05 ng PFOS exposure group and significantly accelerated in the 5 ng exposure group compare with those in control. The adipocyte areas were significantly increased in 0.05 and 0.5 ng per embryo PFOS exposure groups comparing to that of the control. The mRNA sequencing analysis on the medaka larvae chromosome gene expressions showed that 24 genes had significantly changed expressions (p < 0.05, FDR < 0.05) including genes involved in the pathways of metabolism and adipogenesis, like g6pca.2 in the 'Insulin signaling pathway' and 'Adipocytokine signaling pathway'. These results suggested that ovo-nanoinjection-mRNA Seq analysis is a reliable method to evaluate and understand the maternal effect of chemicals.
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M3 - Article
AN - SCOPUS:85098704656
VL - 65
SP - 295
EP - 303
JO - Journal of the Faculty of Agriculture, Kyushu University
JF - Journal of the Faculty of Agriculture, Kyushu University
SN - 0023-6152
IS - 2
ER -