Adiponectin inhibits induction of TNF-α/RANKL-stimulated NFATc1 via the AMPK signaling

Noboru Yamaguchi, Toshio Kukita, Yin Ji Li, Noriaki Kamio, Satoshi Fukumoto, Kazuaki Nonaka, Yuzo Ninomiya, Shigemasa Hanazawa, Yoshihisa Yamashita

Research output: Contribution to journalArticle

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Abstract

We investigated here whether adiponectin can exhibit an inhibitory effect on tumor necrosis factor-alpha (TNF-α)- and receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclastogenesis by using RAW264 cell D clone with a high efficiency to form osteoclasts. Globular adiponectin (gAd) strongly inhibited TNF-α/RANKL-induced differentiation of osteoclasts by interfering with TNF receptor-associated factor 6 production and calcium signaling; consequently, the induction of nuclear factor of activated T cells c1 (NFATc1) was strongly inhibited. Moreover, we observed that inhibition of AMP-activated protein kinase abrogated gAd inhibition for TNF-α/RANKL-induced NFATc1 expression. Our data suggest that adiponectin acts as a potent regulator of bone resorption observed in diseases associated with cytokine activation.

Original languageEnglish
Pages (from-to)451-456
Number of pages6
JournalFEBS Letters
Volume582
Issue number3
DOIs
Publication statusPublished - Feb 6 2008

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NFATC Transcription Factors
AMP-Activated Protein Kinases
Adiponectin
Tumor Necrosis Factor-alpha
Osteoclasts
TNF Receptor-Associated Factor 6
Calcium Signaling
Tumor Necrosis Factor Receptors
Bone Resorption
Osteogenesis
Bone
Clone Cells
Chemical activation
Cytokines
Ligands
Calcium

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Adiponectin inhibits induction of TNF-α/RANKL-stimulated NFATc1 via the AMPK signaling. / Yamaguchi, Noboru; Kukita, Toshio; Li, Yin Ji; Kamio, Noriaki; Fukumoto, Satoshi; Nonaka, Kazuaki; Ninomiya, Yuzo; Hanazawa, Shigemasa; Yamashita, Yoshihisa.

In: FEBS Letters, Vol. 582, No. 3, 06.02.2008, p. 451-456.

Research output: Contribution to journalArticle

Yamaguchi, Noboru ; Kukita, Toshio ; Li, Yin Ji ; Kamio, Noriaki ; Fukumoto, Satoshi ; Nonaka, Kazuaki ; Ninomiya, Yuzo ; Hanazawa, Shigemasa ; Yamashita, Yoshihisa. / Adiponectin inhibits induction of TNF-α/RANKL-stimulated NFATc1 via the AMPK signaling. In: FEBS Letters. 2008 ; Vol. 582, No. 3. pp. 451-456.
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AU - Fukumoto, Satoshi

AU - Nonaka, Kazuaki

AU - Ninomiya, Yuzo

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