Adipose differentiation related protein induces lipid accumulation and lipid droplet formation in hepatic stellate cells

Marie Fukushima, Munechika Enjoji, Motoyuki Kohjima, Rie Sugimoto, Satoshi Ohta, Kazuhiro Kotoh, Masami Kuniyoshi, Kunihisa Kobayashi, Minako Imamura, Toyoshi Inoguchi, Makoto Nakamuta, Hajime Nawata

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The function of adipose differentiation-related protein (ADRP) is known to be the uptake of long-chain fatty acids and formation of lipid droplets in lipid-accumulating cells. We hypothesized that ADRP might stimulate activated hepatic stellate cells (HSCs) to accumulate lipids, resulting in their transition to the quiescent state. In this study, cultured HSCs in fifth passages isolated from rat were infected by adenovirus vector expressing ADRP (Ad.GFP-ADRP), and morphologic and functional changes were evaluated in comparison with control HSCs infected by recombinant adenovirus-expressing β-galactosidase (Ad.LacZ). In Ad.GFP-ADRP-infected cells only, many tiny lipid droplets were apparent in the cytoplasm, while the outline of the cells was not changed. The ADRP was detected around the lipid droplets. In HSCs with intracellular actin filaments, the staining pattern of the filaments before and after infection with Ad.GFP-ADRP or Ad.LacZ did not differ. The cell proliferation rate was not influenced by infection with Ad.LacZ or Ad.GFP-ADRP. Type I collagen secretion from cells overexpressing ADRP was not significantly different from that of Ad.LacZ-infected cells. In our in vitro study, ADRP overexpression induced the formation of cytoplasmic lipid droplets in activated HSCs but could not convert other characteristics of the activated form into those of the quiescent form.

Original languageEnglish
Pages (from-to)321-324
Number of pages4
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume41
Issue number10
DOIs
Publication statusPublished - Nov 1 2005

Fingerprint

Hepatic Stellate Cells
Lipids
Adenoviridae
Perilipin-2
Lipid Droplets
Galactosidases
Collagen Type I
Infection
Actin Cytoskeleton
Cultured Cells
Cytoplasm
Fatty Acids
Cell Proliferation
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

Cite this

Adipose differentiation related protein induces lipid accumulation and lipid droplet formation in hepatic stellate cells. / Fukushima, Marie; Enjoji, Munechika; Kohjima, Motoyuki; Sugimoto, Rie; Ohta, Satoshi; Kotoh, Kazuhiro; Kuniyoshi, Masami; Kobayashi, Kunihisa; Imamura, Minako; Inoguchi, Toyoshi; Nakamuta, Makoto; Nawata, Hajime.

In: In Vitro Cellular and Developmental Biology - Animal, Vol. 41, No. 10, 01.11.2005, p. 321-324.

Research output: Contribution to journalArticle

Fukushima, M, Enjoji, M, Kohjima, M, Sugimoto, R, Ohta, S, Kotoh, K, Kuniyoshi, M, Kobayashi, K, Imamura, M, Inoguchi, T, Nakamuta, M & Nawata, H 2005, 'Adipose differentiation related protein induces lipid accumulation and lipid droplet formation in hepatic stellate cells', In Vitro Cellular and Developmental Biology - Animal, vol. 41, no. 10, pp. 321-324. https://doi.org/10.1290/0410069.1
Fukushima, Marie ; Enjoji, Munechika ; Kohjima, Motoyuki ; Sugimoto, Rie ; Ohta, Satoshi ; Kotoh, Kazuhiro ; Kuniyoshi, Masami ; Kobayashi, Kunihisa ; Imamura, Minako ; Inoguchi, Toyoshi ; Nakamuta, Makoto ; Nawata, Hajime. / Adipose differentiation related protein induces lipid accumulation and lipid droplet formation in hepatic stellate cells. In: In Vitro Cellular and Developmental Biology - Animal. 2005 ; Vol. 41, No. 10. pp. 321-324.
@article{94899d2eef204494b4f1a9cda36fe47a,
title = "Adipose differentiation related protein induces lipid accumulation and lipid droplet formation in hepatic stellate cells",
abstract = "The function of adipose differentiation-related protein (ADRP) is known to be the uptake of long-chain fatty acids and formation of lipid droplets in lipid-accumulating cells. We hypothesized that ADRP might stimulate activated hepatic stellate cells (HSCs) to accumulate lipids, resulting in their transition to the quiescent state. In this study, cultured HSCs in fifth passages isolated from rat were infected by adenovirus vector expressing ADRP (Ad.GFP-ADRP), and morphologic and functional changes were evaluated in comparison with control HSCs infected by recombinant adenovirus-expressing β-galactosidase (Ad.LacZ). In Ad.GFP-ADRP-infected cells only, many tiny lipid droplets were apparent in the cytoplasm, while the outline of the cells was not changed. The ADRP was detected around the lipid droplets. In HSCs with intracellular actin filaments, the staining pattern of the filaments before and after infection with Ad.GFP-ADRP or Ad.LacZ did not differ. The cell proliferation rate was not influenced by infection with Ad.LacZ or Ad.GFP-ADRP. Type I collagen secretion from cells overexpressing ADRP was not significantly different from that of Ad.LacZ-infected cells. In our in vitro study, ADRP overexpression induced the formation of cytoplasmic lipid droplets in activated HSCs but could not convert other characteristics of the activated form into those of the quiescent form.",
author = "Marie Fukushima and Munechika Enjoji and Motoyuki Kohjima and Rie Sugimoto and Satoshi Ohta and Kazuhiro Kotoh and Masami Kuniyoshi and Kunihisa Kobayashi and Minako Imamura and Toyoshi Inoguchi and Makoto Nakamuta and Hajime Nawata",
year = "2005",
month = "11",
day = "1",
doi = "10.1290/0410069.1",
language = "English",
volume = "41",
pages = "321--324",
journal = "In Vitro Cellular and Developmental Biology - Animal",
issn = "1071-2690",
publisher = "Springer New York",
number = "10",

}

TY - JOUR

T1 - Adipose differentiation related protein induces lipid accumulation and lipid droplet formation in hepatic stellate cells

AU - Fukushima, Marie

AU - Enjoji, Munechika

AU - Kohjima, Motoyuki

AU - Sugimoto, Rie

AU - Ohta, Satoshi

AU - Kotoh, Kazuhiro

AU - Kuniyoshi, Masami

AU - Kobayashi, Kunihisa

AU - Imamura, Minako

AU - Inoguchi, Toyoshi

AU - Nakamuta, Makoto

AU - Nawata, Hajime

PY - 2005/11/1

Y1 - 2005/11/1

N2 - The function of adipose differentiation-related protein (ADRP) is known to be the uptake of long-chain fatty acids and formation of lipid droplets in lipid-accumulating cells. We hypothesized that ADRP might stimulate activated hepatic stellate cells (HSCs) to accumulate lipids, resulting in their transition to the quiescent state. In this study, cultured HSCs in fifth passages isolated from rat were infected by adenovirus vector expressing ADRP (Ad.GFP-ADRP), and morphologic and functional changes were evaluated in comparison with control HSCs infected by recombinant adenovirus-expressing β-galactosidase (Ad.LacZ). In Ad.GFP-ADRP-infected cells only, many tiny lipid droplets were apparent in the cytoplasm, while the outline of the cells was not changed. The ADRP was detected around the lipid droplets. In HSCs with intracellular actin filaments, the staining pattern of the filaments before and after infection with Ad.GFP-ADRP or Ad.LacZ did not differ. The cell proliferation rate was not influenced by infection with Ad.LacZ or Ad.GFP-ADRP. Type I collagen secretion from cells overexpressing ADRP was not significantly different from that of Ad.LacZ-infected cells. In our in vitro study, ADRP overexpression induced the formation of cytoplasmic lipid droplets in activated HSCs but could not convert other characteristics of the activated form into those of the quiescent form.

AB - The function of adipose differentiation-related protein (ADRP) is known to be the uptake of long-chain fatty acids and formation of lipid droplets in lipid-accumulating cells. We hypothesized that ADRP might stimulate activated hepatic stellate cells (HSCs) to accumulate lipids, resulting in their transition to the quiescent state. In this study, cultured HSCs in fifth passages isolated from rat were infected by adenovirus vector expressing ADRP (Ad.GFP-ADRP), and morphologic and functional changes were evaluated in comparison with control HSCs infected by recombinant adenovirus-expressing β-galactosidase (Ad.LacZ). In Ad.GFP-ADRP-infected cells only, many tiny lipid droplets were apparent in the cytoplasm, while the outline of the cells was not changed. The ADRP was detected around the lipid droplets. In HSCs with intracellular actin filaments, the staining pattern of the filaments before and after infection with Ad.GFP-ADRP or Ad.LacZ did not differ. The cell proliferation rate was not influenced by infection with Ad.LacZ or Ad.GFP-ADRP. Type I collagen secretion from cells overexpressing ADRP was not significantly different from that of Ad.LacZ-infected cells. In our in vitro study, ADRP overexpression induced the formation of cytoplasmic lipid droplets in activated HSCs but could not convert other characteristics of the activated form into those of the quiescent form.

UR - http://www.scopus.com/inward/record.url?scp=33645016805&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645016805&partnerID=8YFLogxK

U2 - 10.1290/0410069.1

DO - 10.1290/0410069.1

M3 - Article

C2 - 16448220

AN - SCOPUS:33645016805

VL - 41

SP - 321

EP - 324

JO - In Vitro Cellular and Developmental Biology - Animal

JF - In Vitro Cellular and Developmental Biology - Animal

SN - 1071-2690

IS - 10

ER -