Adipose tissue complement factor B promotes adipocyte maturation

Hiroaki Matsunaga, Misaki Iwashita, Takanori Shinjo, Akiko Yamashita, Mitsudai Tsuruta, Shoichiro Nagasaka, Ataru Taniguchi, Mitsuo Fukushima, Naoya Watanabe, Fusanori Nishimura

Research output: Contribution to journalArticle

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Abstract

Objectives It is well-known that the complement system plays an essential role in host immunity. Observational studies have indicated that complement system-related molecules such as complement factor B (CfB) and other components are correlated with obesity and/or insulin resistance parameters. In this study, we investigated the role of adipocyte-derived CfB in adipose tissue metabolism. Methods We investigated the expression level of complement system-related genes in adipocytes. To understand the role of CfB in adipocyte, we performed Cfb overexpression in 3T3-L1 preadipocytes and generated adipocyte-specific Cfb transgenic mice. Results Cfb expression was markedly enhanced in 3T3-L1 adipocytes co-cultured with macrophages following endotoxin stimulation. In Cfb-overexpressing cells, the expression of adipocyte differentiation/maturation-related genes encoding peroxisome proliferator-activated receptor γ (Pparγ), adipocyte Protein 2 and perilipin was significantly enhanced. Cfb transgenic mice showed a marked increase in the expression of genes encoding Pparγ, perilipin, sterol regulatory element-binding protein 1 c, and Cd36 in the subcutaneous adipose tissue. Conclusions CfB plays a crucial role in late-phase of adipocyte differentiation and subsequent lipid droplet formation.

Original languageEnglish
Pages (from-to)740-748
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume495
Issue number1
DOIs
Publication statusPublished - Jan 1 2018

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Complement Factor B
Thromboplastin
Adipocytes
Adipose Tissue
Tissue
Peroxisome Proliferator-Activated Receptors
Gene encoding
Sterol Regulatory Element Binding Protein 1
Macrophages
Metabolism
Endotoxins
Transgenic Mice
Genes
Insulin
Lipids
Molecules
Subcutaneous Fat
Observational Studies
Insulin Resistance
Immunity

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Adipose tissue complement factor B promotes adipocyte maturation. / Matsunaga, Hiroaki; Iwashita, Misaki; Shinjo, Takanori; Yamashita, Akiko; Tsuruta, Mitsudai; Nagasaka, Shoichiro; Taniguchi, Ataru; Fukushima, Mitsuo; Watanabe, Naoya; Nishimura, Fusanori.

In: Biochemical and Biophysical Research Communications, Vol. 495, No. 1, 01.01.2018, p. 740-748.

Research output: Contribution to journalArticle

Matsunaga, Hiroaki ; Iwashita, Misaki ; Shinjo, Takanori ; Yamashita, Akiko ; Tsuruta, Mitsudai ; Nagasaka, Shoichiro ; Taniguchi, Ataru ; Fukushima, Mitsuo ; Watanabe, Naoya ; Nishimura, Fusanori. / Adipose tissue complement factor B promotes adipocyte maturation. In: Biochemical and Biophysical Research Communications. 2018 ; Vol. 495, No. 1. pp. 740-748.
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