TY - JOUR
T1 - Adipose Tissue-specific Expression of the Obese (ob) Gene in Rats and Its Marked Augmentation in Genetically Obese-hyperglycemic Wistar Fatty Rats
AU - Masuzaki, Hiroaki
AU - Ogawa, Yoshihiro
AU - Shigemoto, Michika
AU - Satoh, Noriko
AU - Mori, Kiyoshi
AU - Tamura, Naohisa
AU - Hosoda, Kiminori
AU - Yoshimasa, Yasunao
AU - Jingami, Hisato
AU - Nakao, Kazuwa
PY - 1995
Y1 - 1995
N2 - The obese (ob) gene has recently been isolated by positional genetics, the mutation of which causes marked obesity and diabetes in mice. In the present study, we elucidated the tissue distribution of the ob gene expression in rats and examined the gene expression in genetically obese-hyperglycemic Wistar fatty rats. Northern blot analysis using the rat ob cDNA probe identified a single mRNA species of 4.5 kilobase in size in the adipose tissue, while no significant amount of ob mRNA was detected in other tissues in Sprague-Dawley and Wistar rats. The ob gene expression occurred in the adipose tissue with region-specificities. The rank order of the ob mRNA level in the adipose tissue was epididymal, retroperitoneal, and pericardial white adipose tissue > mesenteric and subcutaneous white adipose tissue ⑥ interscapular brown adipose tissue in both rat strains. Furthermore, expression of the ob gene was augmented in all the adipose tissue examined in Wistar fatty rats at the stage of established obesity.
AB - The obese (ob) gene has recently been isolated by positional genetics, the mutation of which causes marked obesity and diabetes in mice. In the present study, we elucidated the tissue distribution of the ob gene expression in rats and examined the gene expression in genetically obese-hyperglycemic Wistar fatty rats. Northern blot analysis using the rat ob cDNA probe identified a single mRNA species of 4.5 kilobase in size in the adipose tissue, while no significant amount of ob mRNA was detected in other tissues in Sprague-Dawley and Wistar rats. The ob gene expression occurred in the adipose tissue with region-specificities. The rank order of the ob mRNA level in the adipose tissue was epididymal, retroperitoneal, and pericardial white adipose tissue > mesenteric and subcutaneous white adipose tissue ⑥ interscapular brown adipose tissue in both rat strains. Furthermore, expression of the ob gene was augmented in all the adipose tissue examined in Wistar fatty rats at the stage of established obesity.
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U2 - 10.2183/pjab.71.148
DO - 10.2183/pjab.71.148
M3 - Article
AN - SCOPUS:21844506138
VL - 71
SP - 148
EP - 152
JO - Proceedings of the Japan Academy Series B: Physical and Biological Sciences
JF - Proceedings of the Japan Academy Series B: Physical and Biological Sciences
SN - 0386-2208
IS - 5
ER -
