Adipose Tissue-specific Expression of the Obese (ob) Gene in Rats and Its Marked Augmentation in Genetically Obese-hyperglycemic Wistar Fatty Rats

Hiroaki Masuzaki, Yoshihiro Ogawa, Michika Shigemoto, Noriko Satoh, Kiyoshi Mori, Naohisa Tamura, Kiminori Hosoda, Yasunao Yoshimasa, Hisato Jingami, Kazuwa Nakao

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Abstract

The obese (ob) gene has recently been isolated by positional genetics, the mutation of which causes marked obesity and diabetes in mice. In the present study, we elucidated the tissue distribution of the ob gene expression in rats and examined the gene expression in genetically obese-hyperglycemic Wistar fatty rats. Northern blot analysis using the rat ob cDNA probe identified a single mRNA species of 4.5 kilobase in size in the adipose tissue, while no significant amount of ob mRNA was detected in other tissues in Sprague-Dawley and Wistar rats. The ob gene expression occurred in the adipose tissue with region-specificities. The rank order of the ob mRNA level in the adipose tissue was epididymal, retroperitoneal, and pericardial white adipose tissue > mesenteric and subcutaneous white adipose tissue ⑥ interscapular brown adipose tissue in both rat strains. Furthermore, expression of the ob gene was augmented in all the adipose tissue examined in Wistar fatty rats at the stage of established obesity.

Original languageEnglish
Pages (from-to)148-152
Number of pages5
JournalProceedings of the Japan Academy Series B: Physical and Biological Sciences
Volume71
Issue number5
DOIs
Publication statusPublished - Jan 1 1995
Externally publishedYes

Fingerprint

adipose tissues
genes
adipose tissue
rats
Adipose Tissue
Wistar Rats
Gene Expression
White Adipose Tissue
augmentation
gene expression
Messenger RNA
obesity
white adipose tissue
Obesity
Brown Adipose Tissue
Subcutaneous Fat
Tissue Distribution
Northern Blotting
Sprague Dawley Rats
brown adipose tissue

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Physics and Astronomy(all)

Cite this

Adipose Tissue-specific Expression of the Obese (ob) Gene in Rats and Its Marked Augmentation in Genetically Obese-hyperglycemic Wistar Fatty Rats. / Masuzaki, Hiroaki; Ogawa, Yoshihiro; Shigemoto, Michika; Satoh, Noriko; Mori, Kiyoshi; Tamura, Naohisa; Hosoda, Kiminori; Yoshimasa, Yasunao; Jingami, Hisato; Nakao, Kazuwa.

In: Proceedings of the Japan Academy Series B: Physical and Biological Sciences, Vol. 71, No. 5, 01.01.1995, p. 148-152.

Research output: Contribution to journalArticle

Masuzaki, Hiroaki ; Ogawa, Yoshihiro ; Shigemoto, Michika ; Satoh, Noriko ; Mori, Kiyoshi ; Tamura, Naohisa ; Hosoda, Kiminori ; Yoshimasa, Yasunao ; Jingami, Hisato ; Nakao, Kazuwa. / Adipose Tissue-specific Expression of the Obese (ob) Gene in Rats and Its Marked Augmentation in Genetically Obese-hyperglycemic Wistar Fatty Rats. In: Proceedings of the Japan Academy Series B: Physical and Biological Sciences. 1995 ; Vol. 71, No. 5. pp. 148-152.
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abstract = "The obese (ob) gene has recently been isolated by positional genetics, the mutation of which causes marked obesity and diabetes in mice. In the present study, we elucidated the tissue distribution of the ob gene expression in rats and examined the gene expression in genetically obese-hyperglycemic Wistar fatty rats. Northern blot analysis using the rat ob cDNA probe identified a single mRNA species of 4.5 kilobase in size in the adipose tissue, while no significant amount of ob mRNA was detected in other tissues in Sprague-Dawley and Wistar rats. The ob gene expression occurred in the adipose tissue with region-specificities. The rank order of the ob mRNA level in the adipose tissue was epididymal, retroperitoneal, and pericardial white adipose tissue > mesenteric and subcutaneous white adipose tissue ⑥ interscapular brown adipose tissue in both rat strains. Furthermore, expression of the ob gene was augmented in all the adipose tissue examined in Wistar fatty rats at the stage of established obesity.",
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