ADRP stimulates lipid accumulation and lipid droplet formation in murine fibroblasts

Minako Imamura, Toyoshi Inoguchi, Shoichiro Ikuyama, Susumu Taniguchi, Kunihisa Kobayashi, Naoki Nakashima, Hajime Nawata

Research output: Contribution to journalArticle

200 Citations (Scopus)

Abstract

Adipose differentiation-related protein (ADRP) is a lipid droplet-associated protein that is expressed early during adipose differentiation. The present study was undertaken to reveal the role of ADRP in adipose differentiation. In murine fibroblasts infected with green fluorescent protein (GFP)-ADRP fusion protein expression adenovirus vector, confocal microscopic analysis showed the number and size of lipid droplets apparently increased comparing with those of control cells. Overexpressed GFP-ADRP were mainly located at the surface of lipid droplets and appeared to be "ring-shaped." Triacylglycerol content was also significantly (P < 0.001) increased in GFP-ADRP-over-expressed cells compared with control cells. ADRP-induced lipid accumulation did not depend on adipocyte-specific gene induction, such as peroxisome proliferator-activated receptor-γ, lipoprotein lipase, or other lipogenic genes, including acyl-CoA synthetase, fatty acid-binding protein, and fatty acid transporter. In conclusion, ADRP stimulated lipid accumulation and lipid droplet formation without induction of other adipocyte-specific genes or other lipogenic genes in murine fibroblasts. The detailed molecular mechanisms of ADRP on lipid accumulation remain to be elucidated.

Original languageEnglish
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume283
Issue number4 46-4
Publication statusPublished - Oct 1 2002

Fingerprint

Fibroblasts
Lipids
Green Fluorescent Proteins
Adipocytes
Genes
Coenzyme A Ligases
Fatty Acid-Binding Proteins
Peroxisome Proliferator-Activated Receptors
Perilipin-2
Lipid Droplets
Lipoprotein Lipase
Adenoviridae
Triglycerides
Fatty Acids
Proteins

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

Cite this

Imamura, M., Inoguchi, T., Ikuyama, S., Taniguchi, S., Kobayashi, K., Nakashima, N., & Nawata, H. (2002). ADRP stimulates lipid accumulation and lipid droplet formation in murine fibroblasts. American Journal of Physiology - Endocrinology and Metabolism, 283(4 46-4).

ADRP stimulates lipid accumulation and lipid droplet formation in murine fibroblasts. / Imamura, Minako; Inoguchi, Toyoshi; Ikuyama, Shoichiro; Taniguchi, Susumu; Kobayashi, Kunihisa; Nakashima, Naoki; Nawata, Hajime.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 283, No. 4 46-4, 01.10.2002.

Research output: Contribution to journalArticle

Imamura, M, Inoguchi, T, Ikuyama, S, Taniguchi, S, Kobayashi, K, Nakashima, N & Nawata, H 2002, 'ADRP stimulates lipid accumulation and lipid droplet formation in murine fibroblasts', American Journal of Physiology - Endocrinology and Metabolism, vol. 283, no. 4 46-4.
Imamura, Minako ; Inoguchi, Toyoshi ; Ikuyama, Shoichiro ; Taniguchi, Susumu ; Kobayashi, Kunihisa ; Nakashima, Naoki ; Nawata, Hajime. / ADRP stimulates lipid accumulation and lipid droplet formation in murine fibroblasts. In: American Journal of Physiology - Endocrinology and Metabolism. 2002 ; Vol. 283, No. 4 46-4.
@article{0acba02d93af4e76b7599440045c55b1,
title = "ADRP stimulates lipid accumulation and lipid droplet formation in murine fibroblasts",
abstract = "Adipose differentiation-related protein (ADRP) is a lipid droplet-associated protein that is expressed early during adipose differentiation. The present study was undertaken to reveal the role of ADRP in adipose differentiation. In murine fibroblasts infected with green fluorescent protein (GFP)-ADRP fusion protein expression adenovirus vector, confocal microscopic analysis showed the number and size of lipid droplets apparently increased comparing with those of control cells. Overexpressed GFP-ADRP were mainly located at the surface of lipid droplets and appeared to be {"}ring-shaped.{"} Triacylglycerol content was also significantly (P < 0.001) increased in GFP-ADRP-over-expressed cells compared with control cells. ADRP-induced lipid accumulation did not depend on adipocyte-specific gene induction, such as peroxisome proliferator-activated receptor-γ, lipoprotein lipase, or other lipogenic genes, including acyl-CoA synthetase, fatty acid-binding protein, and fatty acid transporter. In conclusion, ADRP stimulated lipid accumulation and lipid droplet formation without induction of other adipocyte-specific genes or other lipogenic genes in murine fibroblasts. The detailed molecular mechanisms of ADRP on lipid accumulation remain to be elucidated.",
author = "Minako Imamura and Toyoshi Inoguchi and Shoichiro Ikuyama and Susumu Taniguchi and Kunihisa Kobayashi and Naoki Nakashima and Hajime Nawata",
year = "2002",
month = "10",
day = "1",
language = "English",
volume = "283",
journal = "American Journal of Physiology - Heart and Circulatory Physiology",
issn = "0363-6135",
publisher = "American Physiological Society",
number = "4 46-4",

}

TY - JOUR

T1 - ADRP stimulates lipid accumulation and lipid droplet formation in murine fibroblasts

AU - Imamura, Minako

AU - Inoguchi, Toyoshi

AU - Ikuyama, Shoichiro

AU - Taniguchi, Susumu

AU - Kobayashi, Kunihisa

AU - Nakashima, Naoki

AU - Nawata, Hajime

PY - 2002/10/1

Y1 - 2002/10/1

N2 - Adipose differentiation-related protein (ADRP) is a lipid droplet-associated protein that is expressed early during adipose differentiation. The present study was undertaken to reveal the role of ADRP in adipose differentiation. In murine fibroblasts infected with green fluorescent protein (GFP)-ADRP fusion protein expression adenovirus vector, confocal microscopic analysis showed the number and size of lipid droplets apparently increased comparing with those of control cells. Overexpressed GFP-ADRP were mainly located at the surface of lipid droplets and appeared to be "ring-shaped." Triacylglycerol content was also significantly (P < 0.001) increased in GFP-ADRP-over-expressed cells compared with control cells. ADRP-induced lipid accumulation did not depend on adipocyte-specific gene induction, such as peroxisome proliferator-activated receptor-γ, lipoprotein lipase, or other lipogenic genes, including acyl-CoA synthetase, fatty acid-binding protein, and fatty acid transporter. In conclusion, ADRP stimulated lipid accumulation and lipid droplet formation without induction of other adipocyte-specific genes or other lipogenic genes in murine fibroblasts. The detailed molecular mechanisms of ADRP on lipid accumulation remain to be elucidated.

AB - Adipose differentiation-related protein (ADRP) is a lipid droplet-associated protein that is expressed early during adipose differentiation. The present study was undertaken to reveal the role of ADRP in adipose differentiation. In murine fibroblasts infected with green fluorescent protein (GFP)-ADRP fusion protein expression adenovirus vector, confocal microscopic analysis showed the number and size of lipid droplets apparently increased comparing with those of control cells. Overexpressed GFP-ADRP were mainly located at the surface of lipid droplets and appeared to be "ring-shaped." Triacylglycerol content was also significantly (P < 0.001) increased in GFP-ADRP-over-expressed cells compared with control cells. ADRP-induced lipid accumulation did not depend on adipocyte-specific gene induction, such as peroxisome proliferator-activated receptor-γ, lipoprotein lipase, or other lipogenic genes, including acyl-CoA synthetase, fatty acid-binding protein, and fatty acid transporter. In conclusion, ADRP stimulated lipid accumulation and lipid droplet formation without induction of other adipocyte-specific genes or other lipogenic genes in murine fibroblasts. The detailed molecular mechanisms of ADRP on lipid accumulation remain to be elucidated.

UR - http://www.scopus.com/inward/record.url?scp=0036786517&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036786517&partnerID=8YFLogxK

M3 - Article

VL - 283

JO - American Journal of Physiology - Heart and Circulatory Physiology

JF - American Journal of Physiology - Heart and Circulatory Physiology

SN - 0363-6135

IS - 4 46-4

ER -