Affinity capillary electrophoresis using DNA conjugates.

Y. Ozaki, T. Ihara, Yoshiki Katayama, M. Maeda

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

An oligonucleotide ((dT)12)-immobilized capillary was prepared for the affinity capillary electrophoresis of DNA. Oligonucleotides ((dA)6 and (dT)6) which had the same chain length but had different base composition were separated in high resolution in the presence of Mg2+ ion. Whereas (dA)6 eluted slightly earlier than (dT)6 in a conventional polyacrylamide-coated capillary, the order of their mobilities was reversed in the (dT)12-modified system, suggesting the complementary hydrogen bondings between (dA)6 and the immobilized (dT)12.

Original languageEnglish
Pages (from-to)235-236
Number of pages2
JournalNucleic acids symposium series
Issue number37
Publication statusPublished - Jan 1 1997

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Capillary Electrophoresis
Oligonucleotides
DNA
Base Composition
Hydrogen Bonding
Ions
polyacrylamide

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Affinity capillary electrophoresis using DNA conjugates. / Ozaki, Y.; Ihara, T.; Katayama, Yoshiki; Maeda, M.

In: Nucleic acids symposium series, No. 37, 01.01.1997, p. 235-236.

Research output: Contribution to journalArticle

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