Affinity capillary electrophoresis using DNA conjugates.

Y. Ozaki; T. Ihara; Yoshiki Katayama; M. Maeda

Affinity capillary electrophoresis using DNA conjugates.

Y. Ozaki, T. Ihara, Yoshiki Katayama, M. Maeda

Research output: Contribution to journal › Article › peer-review

Abstract

An oligonucleotide ((dT)12)-immobilized capillary was prepared for the affinity capillary electrophoresis of DNA. Oligonucleotides ((dA)6 and (dT)6) which had the same chain length but had different base composition were separated in high resolution in the presence of Mg2+ ion. Whereas (dA)6 eluted slightly earlier than (dT)6 in a conventional polyacrylamide-coated capillary, the order of their mobilities was reversed in the (dT)12-modified system, suggesting the complementary hydrogen bondings between (dA)6 and the immobilized (dT)12.

Original language	English
Pages (from-to)	235-236
Number of pages	2
Journal	Nucleic acids symposium series
Issue number	37
Publication status	Published - Jan 1 1997

All Science Journal Classification (ASJC) codes

Medicine(all)

Cite this

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AU - Katayama, Yoshiki

AU - Maeda, M.

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N2 - An oligonucleotide ((dT)12)-immobilized capillary was prepared for the affinity capillary electrophoresis of DNA. Oligonucleotides ((dA)6 and (dT)6) which had the same chain length but had different base composition were separated in high resolution in the presence of Mg2+ ion. Whereas (dA)6 eluted slightly earlier than (dT)6 in a conventional polyacrylamide-coated capillary, the order of their mobilities was reversed in the (dT)12-modified system, suggesting the complementary hydrogen bondings between (dA)6 and the immobilized (dT)12.

AB - An oligonucleotide ((dT)12)-immobilized capillary was prepared for the affinity capillary electrophoresis of DNA. Oligonucleotides ((dA)6 and (dT)6) which had the same chain length but had different base composition were separated in high resolution in the presence of Mg2+ ion. Whereas (dA)6 eluted slightly earlier than (dT)6 in a conventional polyacrylamide-coated capillary, the order of their mobilities was reversed in the (dT)12-modified system, suggesting the complementary hydrogen bondings between (dA)6 and the immobilized (dT)12.

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JF - Nucleic acids symposium series

IS - 37

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Affinity capillary electrophoresis using DNA conjugates.

Abstract

All Science Journal Classification (ASJC) codes

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