Affinity purification of multifunctional polymer nanoparticles

Yu Hoshino, Walter W. Haberaecker, Takashi Kodama, Zhiyang Zeng, Yoshio Okahata, Kenneth J. Shea

Research output: Contribution to journalArticlepeer-review

68 Citations (Scopus)

Abstract

We report that multifunctional polymer nanoparticles approximately the size of a large protein can be 'purified', on the basis of peptide affinity just as antibodies, using an affinity chromatography strategy. The selection process takes advantage of the thermoresponsiveness of the nanoparticles allowing 'catch and release' of the target peptide by adjusting the temperature. Purified particles show much stronger affinity (Kdapp ≈ nM) and a narrower affinity distribution than the average of particles before purification (Kdapp > μM) at room temperature but can release the peptide just by changing the temperature. We anticipate this affinity selection will be general and become an integral step for the preparation of "plastic antibodies" with near-homogeneous and tailored affinity for target biomacromolecules.

Original languageEnglish
Pages (from-to)13648-13650
Number of pages3
JournalJournal of the American Chemical Society
Volume132
Issue number39
DOIs
Publication statusPublished - Oct 6 2010
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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