Age-associated increase in the expression of T-cell antigen receptor γ-chain gene in conventional and germfree mice

Y. Yoshikai, G. Matsuzaki, K. Kishihara, K. Nomoto, T. Yokokura, K. Nomoto

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    To determine whether environmental antigens such as intestinal microflora contribute to expansion of the T-cell repertoire, age-related changes in the expression level of T-cell antigen receptor genes encoding γ, β, and α chains were compared in the lymphoid tissues of conventional versus germfree mice. Irrespective of the conditions of maintenance, an age-associated increase in the expression of the γ-chain gene was evident in the thymus and spleen. Both conventional and germfree old mice (age, 40 weeks) had a relatively high proportion of Thy1+ L3T4- Lyt2- cells but a reduced level of Thy1+ L3T4- Lyt2+ cells in the thymus compared with their counterparts (age 8 weeks). The thymic dysfunction but not the stimulation by intestinal microflora may contribute to this age-related increase in γ-gene transcripts in these tissues. On the other hand, an age-associated increase in the expression of γ RNA was not evident in the mesenteric lymph nodes of germfree mice, although a remarkable increase in the γ-chain gene messages was detected in the lymph nodes of the aged conventional mice. These results suggest that the expression of γ RNA in cells of gut-associated lymphoid tissue is partly influenced by intestinal microflora.

    Original languageEnglish
    Pages (from-to)2069-2074
    Number of pages6
    JournalInfection and Immunity
    Volume56
    Issue number8
    Publication statusPublished - Jan 1 1988

    Fingerprint

    T-Cell Antigen Receptor
    Lymphoid Tissue
    Thymus Gland
    Genes
    Lymph Nodes
    RNA
    T-Cell Receptor Genes
    Spleen
    Maintenance
    T-Lymphocytes
    Gene Expression
    Antigens
    Gastrointestinal Microbiome

    All Science Journal Classification (ASJC) codes

    • Parasitology
    • Microbiology
    • Immunology
    • Infectious Diseases

    Cite this

    Yoshikai, Y., Matsuzaki, G., Kishihara, K., Nomoto, K., Yokokura, T., & Nomoto, K. (1988). Age-associated increase in the expression of T-cell antigen receptor γ-chain gene in conventional and germfree mice. Infection and Immunity, 56(8), 2069-2074.

    Age-associated increase in the expression of T-cell antigen receptor γ-chain gene in conventional and germfree mice. / Yoshikai, Y.; Matsuzaki, G.; Kishihara, K.; Nomoto, K.; Yokokura, T.; Nomoto, K.

    In: Infection and Immunity, Vol. 56, No. 8, 01.01.1988, p. 2069-2074.

    Research output: Contribution to journalArticle

    Yoshikai, Y, Matsuzaki, G, Kishihara, K, Nomoto, K, Yokokura, T & Nomoto, K 1988, 'Age-associated increase in the expression of T-cell antigen receptor γ-chain gene in conventional and germfree mice', Infection and Immunity, vol. 56, no. 8, pp. 2069-2074.
    Yoshikai Y, Matsuzaki G, Kishihara K, Nomoto K, Yokokura T, Nomoto K. Age-associated increase in the expression of T-cell antigen receptor γ-chain gene in conventional and germfree mice. Infection and Immunity. 1988 Jan 1;56(8):2069-2074.
    Yoshikai, Y. ; Matsuzaki, G. ; Kishihara, K. ; Nomoto, K. ; Yokokura, T. ; Nomoto, K. / Age-associated increase in the expression of T-cell antigen receptor γ-chain gene in conventional and germfree mice. In: Infection and Immunity. 1988 ; Vol. 56, No. 8. pp. 2069-2074.
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