Age related changes in the localization of glycosaminoglycans in condylar cartilage of the mandible in rats

I. Takahashi, I. Mizoguchi, Y. Sasano, S. Saitoh, M. Ishida, M. Kagayama, H. Mitani

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

There is little information available regarding the morphological and biomolecular characteristics of mandibular condylar cartilage. The purpose of this study was to determine the age-related changes in the morphology and immunolocalization of glycosaminoglycans (GAGs) in mandibular condyles. The mandibular condylar cartilages from 4-, 8-, 16-, 32-, and 64-week-old Wistar male rats were examined to verify the localization of chondroitin-4-sulfate (Ch-4S), chondroitin-6-sulfate (Ch-6S) and keratan sulfate (KS) using an indirect immuno-fluorescent technique with three monoclonal antibodies for glycosaminoglycans, 2-B-6, 3-B-3 and 5-D-4, respectively. Morphologically, the condylar cartilage was a growth cartilage during growing periods, began to differentiate into articular cartilage from the central area of 16-week-old condyles, and became mature articular cartilage at 32 weeks of age. A regional difference was found in the morphological features and distribution of GAGs between the anterior, central, postero-superior and posterior areas of the condyles at each age. The immunohistochemical localizations of these three glycosaminoglycans showed age-related, morphology-dependent changes, from growth cartilage to articular cartilage-like cartilage. Immunoreactions for all of the antibodies decreased progressively with age in the interterritorial matrix, while the pericellular and territorial matrix in the condylar cartilage of the mandible maintained relatively higher immunoreactivity. In conclusion, age-related and regional differences in the localization of glycosaminoglycans Ch-4S, Ch-6S, and KS were found in the mandibular condyles in rats, and these changes are believed to be related to functional and developmental requirements.

Original languageEnglish
Pages (from-to)489-500
Number of pages12
JournalAnatomy and Embryology
Volume194
Issue number5
DOIs
Publication statusPublished - Jan 1 1996

Fingerprint

Glycosaminoglycans
Mandible
Cartilage
Chondroitin Sulfates
Articular Cartilage
Keratan Sulfate
Mandibular Condyle
Bone and Bones
Growth
Wistar Rats
Monoclonal Antibodies
Antibodies

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Embryology
  • Developmental Biology
  • Cell Biology

Cite this

Age related changes in the localization of glycosaminoglycans in condylar cartilage of the mandible in rats. / Takahashi, I.; Mizoguchi, I.; Sasano, Y.; Saitoh, S.; Ishida, M.; Kagayama, M.; Mitani, H.

In: Anatomy and Embryology, Vol. 194, No. 5, 01.01.1996, p. 489-500.

Research output: Contribution to journalArticle

Takahashi, I. ; Mizoguchi, I. ; Sasano, Y. ; Saitoh, S. ; Ishida, M. ; Kagayama, M. ; Mitani, H. / Age related changes in the localization of glycosaminoglycans in condylar cartilage of the mandible in rats. In: Anatomy and Embryology. 1996 ; Vol. 194, No. 5. pp. 489-500.
@article{40129f74975a4dff8390c7d2dd27350c,
title = "Age related changes in the localization of glycosaminoglycans in condylar cartilage of the mandible in rats",
abstract = "There is little information available regarding the morphological and biomolecular characteristics of mandibular condylar cartilage. The purpose of this study was to determine the age-related changes in the morphology and immunolocalization of glycosaminoglycans (GAGs) in mandibular condyles. The mandibular condylar cartilages from 4-, 8-, 16-, 32-, and 64-week-old Wistar male rats were examined to verify the localization of chondroitin-4-sulfate (Ch-4S), chondroitin-6-sulfate (Ch-6S) and keratan sulfate (KS) using an indirect immuno-fluorescent technique with three monoclonal antibodies for glycosaminoglycans, 2-B-6, 3-B-3 and 5-D-4, respectively. Morphologically, the condylar cartilage was a growth cartilage during growing periods, began to differentiate into articular cartilage from the central area of 16-week-old condyles, and became mature articular cartilage at 32 weeks of age. A regional difference was found in the morphological features and distribution of GAGs between the anterior, central, postero-superior and posterior areas of the condyles at each age. The immunohistochemical localizations of these three glycosaminoglycans showed age-related, morphology-dependent changes, from growth cartilage to articular cartilage-like cartilage. Immunoreactions for all of the antibodies decreased progressively with age in the interterritorial matrix, while the pericellular and territorial matrix in the condylar cartilage of the mandible maintained relatively higher immunoreactivity. In conclusion, age-related and regional differences in the localization of glycosaminoglycans Ch-4S, Ch-6S, and KS were found in the mandibular condyles in rats, and these changes are believed to be related to functional and developmental requirements.",
author = "I. Takahashi and I. Mizoguchi and Y. Sasano and S. Saitoh and M. Ishida and M. Kagayama and H. Mitani",
year = "1996",
month = "1",
day = "1",
doi = "10.1007/BF00185995",
language = "English",
volume = "194",
pages = "489--500",
journal = "Anatomy and Embryology",
issn = "0340-2061",
number = "5",

}

TY - JOUR

T1 - Age related changes in the localization of glycosaminoglycans in condylar cartilage of the mandible in rats

AU - Takahashi, I.

AU - Mizoguchi, I.

AU - Sasano, Y.

AU - Saitoh, S.

AU - Ishida, M.

AU - Kagayama, M.

AU - Mitani, H.

PY - 1996/1/1

Y1 - 1996/1/1

N2 - There is little information available regarding the morphological and biomolecular characteristics of mandibular condylar cartilage. The purpose of this study was to determine the age-related changes in the morphology and immunolocalization of glycosaminoglycans (GAGs) in mandibular condyles. The mandibular condylar cartilages from 4-, 8-, 16-, 32-, and 64-week-old Wistar male rats were examined to verify the localization of chondroitin-4-sulfate (Ch-4S), chondroitin-6-sulfate (Ch-6S) and keratan sulfate (KS) using an indirect immuno-fluorescent technique with three monoclonal antibodies for glycosaminoglycans, 2-B-6, 3-B-3 and 5-D-4, respectively. Morphologically, the condylar cartilage was a growth cartilage during growing periods, began to differentiate into articular cartilage from the central area of 16-week-old condyles, and became mature articular cartilage at 32 weeks of age. A regional difference was found in the morphological features and distribution of GAGs between the anterior, central, postero-superior and posterior areas of the condyles at each age. The immunohistochemical localizations of these three glycosaminoglycans showed age-related, morphology-dependent changes, from growth cartilage to articular cartilage-like cartilage. Immunoreactions for all of the antibodies decreased progressively with age in the interterritorial matrix, while the pericellular and territorial matrix in the condylar cartilage of the mandible maintained relatively higher immunoreactivity. In conclusion, age-related and regional differences in the localization of glycosaminoglycans Ch-4S, Ch-6S, and KS were found in the mandibular condyles in rats, and these changes are believed to be related to functional and developmental requirements.

AB - There is little information available regarding the morphological and biomolecular characteristics of mandibular condylar cartilage. The purpose of this study was to determine the age-related changes in the morphology and immunolocalization of glycosaminoglycans (GAGs) in mandibular condyles. The mandibular condylar cartilages from 4-, 8-, 16-, 32-, and 64-week-old Wistar male rats were examined to verify the localization of chondroitin-4-sulfate (Ch-4S), chondroitin-6-sulfate (Ch-6S) and keratan sulfate (KS) using an indirect immuno-fluorescent technique with three monoclonal antibodies for glycosaminoglycans, 2-B-6, 3-B-3 and 5-D-4, respectively. Morphologically, the condylar cartilage was a growth cartilage during growing periods, began to differentiate into articular cartilage from the central area of 16-week-old condyles, and became mature articular cartilage at 32 weeks of age. A regional difference was found in the morphological features and distribution of GAGs between the anterior, central, postero-superior and posterior areas of the condyles at each age. The immunohistochemical localizations of these three glycosaminoglycans showed age-related, morphology-dependent changes, from growth cartilage to articular cartilage-like cartilage. Immunoreactions for all of the antibodies decreased progressively with age in the interterritorial matrix, while the pericellular and territorial matrix in the condylar cartilage of the mandible maintained relatively higher immunoreactivity. In conclusion, age-related and regional differences in the localization of glycosaminoglycans Ch-4S, Ch-6S, and KS were found in the mandibular condyles in rats, and these changes are believed to be related to functional and developmental requirements.

UR - http://www.scopus.com/inward/record.url?scp=0029851807&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029851807&partnerID=8YFLogxK

U2 - 10.1007/BF00185995

DO - 10.1007/BF00185995

M3 - Article

C2 - 8905015

AN - SCOPUS:0029851807

VL - 194

SP - 489

EP - 500

JO - Anatomy and Embryology

JF - Anatomy and Embryology

SN - 0340-2061

IS - 5

ER -