Age‐associated increase in number of CD4+CD8+ intestinal intraepithelial lymphocytes in rats

Hiroaki Takimoto, Takao Nakamura, Makoto Takeuchi, Yukiko Sumi, Toshiyuki Tanaka, Kikuo Nomoto, Yasunobu Yoshikai

    Research output: Contribution to journalArticle

    72 Citations (Scopus)

    Abstract

    A significant number of CD4+CD8+ T cells were detected in intestinal intraepithelial lymphocytes (IEL) of various strains of rats including Wistar, WKA, BN, LEW and F344. The site of the CD4+CD8+ population in IEL increased with age in all strains we examined. Most IEL bearing CD8 expressed no CD5 antigen in young rats, while all CD4+CD8+ IEL and some of CD8+ IEL in aged rats were of CD5+CD45RB phenotype. In germ‐free Wistar rats, age‐associated increase in the number of CD4+CD8+CD5+ IEL was not evident, indicating that stimulation by the intestinal microflora was important for expansion of the CD4+CD8+CD5+CD45RB IEL. Aged athymic F344 nude rats contained appreciable numbers of CD4+ IEL and CD8+ IEL but few CD4+CD8+ IEL, suggesting that the CD4+CD8+ IEL may be derived from thymus‐dependent populations. Unlike a majority of CD4+CD8+ thymocytes bearing a low intensity of CD3/T cell receptor (TcR) α/β, the CD4+CD8+ T cells in IEL expressed a high intensity of CD3/TcR α/β on their surface. The CD4+CD8+ IEL appear to contribute to the spontaneous proliferation of the IEL in aged rats as assessed by tritiated thymidine incorporation after in vitro culture with medium only. These results suggest that with aging a unique CD4+CD8+ IEL may expand at a local site of the intestine under the influence of intestinal microflora and may contribute to the first line of defense against various pathogens in the epithelium.

    Original languageEnglish
    Pages (from-to)159-164
    Number of pages6
    JournalEuropean Journal of Immunology
    Volume22
    Issue number1
    DOIs
    Publication statusPublished - Jan 1 1992

    Fingerprint

    Lymphocytes
    Nude Rats
    T-Cell Antigen Receptor
    Wistar Rats
    CD5 Antigens
    T-Lymphocytes
    Inbred F344 Rats
    Thymocytes
    Thymidine
    Population
    Intestines
    Culture Media
    Epithelium
    Phenotype

    All Science Journal Classification (ASJC) codes

    • Immunology and Allergy
    • Immunology

    Cite this

    Takimoto, H., Nakamura, T., Takeuchi, M., Sumi, Y., Tanaka, T., Nomoto, K., & Yoshikai, Y. (1992). Age‐associated increase in number of CD4+CD8+ intestinal intraepithelial lymphocytes in rats. European Journal of Immunology, 22(1), 159-164. https://doi.org/10.1002/eji.1830220124

    Age‐associated increase in number of CD4+CD8+ intestinal intraepithelial lymphocytes in rats. / Takimoto, Hiroaki; Nakamura, Takao; Takeuchi, Makoto; Sumi, Yukiko; Tanaka, Toshiyuki; Nomoto, Kikuo; Yoshikai, Yasunobu.

    In: European Journal of Immunology, Vol. 22, No. 1, 01.01.1992, p. 159-164.

    Research output: Contribution to journalArticle

    Takimoto, H, Nakamura, T, Takeuchi, M, Sumi, Y, Tanaka, T, Nomoto, K & Yoshikai, Y 1992, 'Age‐associated increase in number of CD4+CD8+ intestinal intraepithelial lymphocytes in rats', European Journal of Immunology, vol. 22, no. 1, pp. 159-164. https://doi.org/10.1002/eji.1830220124
    Takimoto, Hiroaki ; Nakamura, Takao ; Takeuchi, Makoto ; Sumi, Yukiko ; Tanaka, Toshiyuki ; Nomoto, Kikuo ; Yoshikai, Yasunobu. / Age‐associated increase in number of CD4+CD8+ intestinal intraepithelial lymphocytes in rats. In: European Journal of Immunology. 1992 ; Vol. 22, No. 1. pp. 159-164.
    @article{dbdd11944d4c4971a3887af816bba14b,
    title = "Age‐associated increase in number of CD4+CD8+ intestinal intraepithelial lymphocytes in rats",
    abstract = "A significant number of CD4+CD8+ T cells were detected in intestinal intraepithelial lymphocytes (IEL) of various strains of rats including Wistar, WKA, BN, LEW and F344. The site of the CD4+CD8+ population in IEL increased with age in all strains we examined. Most IEL bearing CD8 expressed no CD5 antigen in young rats, while all CD4+CD8+ IEL and some of CD8+ IEL in aged rats were of CD5+CD45RB− phenotype. In germ‐free Wistar rats, age‐associated increase in the number of CD4+CD8+CD5+ IEL was not evident, indicating that stimulation by the intestinal microflora was important for expansion of the CD4+CD8+CD5+CD45RB− IEL. Aged athymic F344 nude rats contained appreciable numbers of CD4+ IEL and CD8+ IEL but few CD4+CD8+ IEL, suggesting that the CD4+CD8+ IEL may be derived from thymus‐dependent populations. Unlike a majority of CD4+CD8+ thymocytes bearing a low intensity of CD3/T cell receptor (TcR) α/β, the CD4+CD8+ T cells in IEL expressed a high intensity of CD3/TcR α/β on their surface. The CD4+CD8+ IEL appear to contribute to the spontaneous proliferation of the IEL in aged rats as assessed by tritiated thymidine incorporation after in vitro culture with medium only. These results suggest that with aging a unique CD4+CD8+ IEL may expand at a local site of the intestine under the influence of intestinal microflora and may contribute to the first line of defense against various pathogens in the epithelium.",
    author = "Hiroaki Takimoto and Takao Nakamura and Makoto Takeuchi and Yukiko Sumi and Toshiyuki Tanaka and Kikuo Nomoto and Yasunobu Yoshikai",
    year = "1992",
    month = "1",
    day = "1",
    doi = "10.1002/eji.1830220124",
    language = "English",
    volume = "22",
    pages = "159--164",
    journal = "European Journal of Immunology",
    issn = "0014-2980",
    publisher = "Wiley-VCH Verlag",
    number = "1",

    }

    TY - JOUR

    T1 - Age‐associated increase in number of CD4+CD8+ intestinal intraepithelial lymphocytes in rats

    AU - Takimoto, Hiroaki

    AU - Nakamura, Takao

    AU - Takeuchi, Makoto

    AU - Sumi, Yukiko

    AU - Tanaka, Toshiyuki

    AU - Nomoto, Kikuo

    AU - Yoshikai, Yasunobu

    PY - 1992/1/1

    Y1 - 1992/1/1

    N2 - A significant number of CD4+CD8+ T cells were detected in intestinal intraepithelial lymphocytes (IEL) of various strains of rats including Wistar, WKA, BN, LEW and F344. The site of the CD4+CD8+ population in IEL increased with age in all strains we examined. Most IEL bearing CD8 expressed no CD5 antigen in young rats, while all CD4+CD8+ IEL and some of CD8+ IEL in aged rats were of CD5+CD45RB− phenotype. In germ‐free Wistar rats, age‐associated increase in the number of CD4+CD8+CD5+ IEL was not evident, indicating that stimulation by the intestinal microflora was important for expansion of the CD4+CD8+CD5+CD45RB− IEL. Aged athymic F344 nude rats contained appreciable numbers of CD4+ IEL and CD8+ IEL but few CD4+CD8+ IEL, suggesting that the CD4+CD8+ IEL may be derived from thymus‐dependent populations. Unlike a majority of CD4+CD8+ thymocytes bearing a low intensity of CD3/T cell receptor (TcR) α/β, the CD4+CD8+ T cells in IEL expressed a high intensity of CD3/TcR α/β on their surface. The CD4+CD8+ IEL appear to contribute to the spontaneous proliferation of the IEL in aged rats as assessed by tritiated thymidine incorporation after in vitro culture with medium only. These results suggest that with aging a unique CD4+CD8+ IEL may expand at a local site of the intestine under the influence of intestinal microflora and may contribute to the first line of defense against various pathogens in the epithelium.

    AB - A significant number of CD4+CD8+ T cells were detected in intestinal intraepithelial lymphocytes (IEL) of various strains of rats including Wistar, WKA, BN, LEW and F344. The site of the CD4+CD8+ population in IEL increased with age in all strains we examined. Most IEL bearing CD8 expressed no CD5 antigen in young rats, while all CD4+CD8+ IEL and some of CD8+ IEL in aged rats were of CD5+CD45RB− phenotype. In germ‐free Wistar rats, age‐associated increase in the number of CD4+CD8+CD5+ IEL was not evident, indicating that stimulation by the intestinal microflora was important for expansion of the CD4+CD8+CD5+CD45RB− IEL. Aged athymic F344 nude rats contained appreciable numbers of CD4+ IEL and CD8+ IEL but few CD4+CD8+ IEL, suggesting that the CD4+CD8+ IEL may be derived from thymus‐dependent populations. Unlike a majority of CD4+CD8+ thymocytes bearing a low intensity of CD3/T cell receptor (TcR) α/β, the CD4+CD8+ T cells in IEL expressed a high intensity of CD3/TcR α/β on their surface. The CD4+CD8+ IEL appear to contribute to the spontaneous proliferation of the IEL in aged rats as assessed by tritiated thymidine incorporation after in vitro culture with medium only. These results suggest that with aging a unique CD4+CD8+ IEL may expand at a local site of the intestine under the influence of intestinal microflora and may contribute to the first line of defense against various pathogens in the epithelium.

    UR - http://www.scopus.com/inward/record.url?scp=0026586373&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0026586373&partnerID=8YFLogxK

    U2 - 10.1002/eji.1830220124

    DO - 10.1002/eji.1830220124

    M3 - Article

    C2 - 1370412

    AN - SCOPUS:0026586373

    VL - 22

    SP - 159

    EP - 164

    JO - European Journal of Immunology

    JF - European Journal of Immunology

    SN - 0014-2980

    IS - 1

    ER -