Agonist-induced changes in Ca2+ permeation through the nociceptor cation channel TRPA1

Yuji Karashima, Jean Prenen, Karel Talavera, Annelies Janssens, Thomas Voets, Bernd Nilius

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Abstract

The Ca2+-permeable cation channel TRPA1 acts as an ionotropic receptor for various pungent compounds and as a noxious cold sensor in sensory neurons. It is unclear what proportion of the TRPA1-mediated current is carried by Ca2+ ions and how the permeation pathway changes during stimulation. Here, based on the relative permeability of the nonstimulated channel to cations of different size, we estimated a pore diameter of ∼11 Å. Combined patch-clamp and Fura-2 fluorescence recordings revealed that with 2 mM extracellular Ca2+, and at a membrane potential of -80 mV, ∼17% of the inward TRPA1 current is carried by Ca2+. Stimulation with mustard oil evoked an apparent dilatation of the pore of 3 Å and an increase in divalent cation selectivity and fractional Ca2+ current. Mutations in the putative pore that reduced the divalent permeability and fractional Ca2+ current also prevented mustard-oil-induced increases in Ca2+ permeation. It is interesting that fractional Ca2+ currents for wild-type and mutant TRPA1 were consistently higher than values predicted based on biionic reversal potentials using the Goldman-Hodgkin-Katz equation, suggesting that binding of Ca2+ in the pore hinders monovalent cation permeation. We conclude that the pore of TRPA1 is dynamic and supports a surprisingly large Ca2+ influx.

Original languageEnglish
Pages (from-to)773-783
Number of pages11
JournalBiophysical Journal
Volume98
Issue number5
DOIs
Publication statusPublished - Mar 3 2010
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Biophysics

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