TY - JOUR
T1 - Agrobacterium-mediated transformation of Euphorbia tirucalli callus
AU - Uchida, Hidenobu
AU - Yamashita, Hirofumi
AU - Anai, Toyoaki
AU - Muranaka, Toshiya
AU - Ohyama, Kanji
N1 - Funding Information:
We thank Dr. Takeshi Ohama and Dr. Masafumi Taniwaki of Kochi University of Technology (KUT), and Dr. Tatsuro Hamada and Dr. Miho Takemura of Ishikawa Prefectural University for encouragement throughout this study. It was partly supported by the KUT Post Doctoral Fellow (Research Associate) Research Promotion Fund (code 2121302) to H.U. (code 2090119). It was partly performed as one of the technology development projects of the ‘‘Green Biotechnology Program,’’ and was supported by a NEDO (New Energy and Industrial Technology Development Organization) grant to K.O.
PY - 2010
Y1 - 2010
N2 - In order to establish a basis for transformation technology in the petroleum plant Euphorbia tirucalli, the callus of the plant was infected with Agrobacterium, washed with distilled water, sterilized with distilled water containing 100 mg/l of carbenicillin, selected on solidified B5 medium containing 13 mg/l of G418 and 100 mg/l of carbenicillin, and then on solidified B5 medium containing 25 mg/l of G418 and 100 mg/l of carbenicillin for the transgenic calli, and then the callus lines were subcultured successively on solidified B5 medium containing 50 mg/l of G418. We performed PCR analysis of sterilized G418-resistant callus line DNA and concluded that the gene introduced was integrated into the callus genome.
AB - In order to establish a basis for transformation technology in the petroleum plant Euphorbia tirucalli, the callus of the plant was infected with Agrobacterium, washed with distilled water, sterilized with distilled water containing 100 mg/l of carbenicillin, selected on solidified B5 medium containing 13 mg/l of G418 and 100 mg/l of carbenicillin, and then on solidified B5 medium containing 25 mg/l of G418 and 100 mg/l of carbenicillin for the transgenic calli, and then the callus lines were subcultured successively on solidified B5 medium containing 50 mg/l of G418. We performed PCR analysis of sterilized G418-resistant callus line DNA and concluded that the gene introduced was integrated into the callus genome.
UR - http://www.scopus.com/inward/record.url?scp=77951524587&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77951524587&partnerID=8YFLogxK
U2 - 10.1271/bbb.90783
DO - 10.1271/bbb.90783
M3 - Article
C2 - 20445322
AN - SCOPUS:77951524587
VL - 74
SP - 851
EP - 853
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 4
ER -