Agrobacterium-mediated transformation of Euphorbia tirucalli callus

Hidenobu Uchida, Hirofumi Yamashita, Toyoaki Anai, Toshiya Muranaka, Kanji Ohyama

Research output: Contribution to journalArticlepeer-review

Abstract

In order to establish a basis for transformation technology in the petroleum plant Euphorbia tirucalli, the callus of the plant was infected with Agrobacterium, washed with distilled water, sterilized with distilled water containing 100 mg/l of carbenicillin, selected on solidified B5 medium containing 13 mg/l of G418 and 100 mg/l of carbenicillin, and then on solidified B5 medium containing 25 mg/l of G418 and 100 mg/l of carbenicillin for the transgenic calli, and then the callus lines were subcultured successively on solidified B5 medium containing 50 mg/l of G418. We performed PCR analysis of sterilized G418-resistant callus line DNA and concluded that the gene introduced was integrated into the callus genome.

Original languageEnglish
Pages (from-to)851-853
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Volume74
Issue number4
DOIs
Publication statusPublished - 2010
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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