Agrobacterium-mediated transformation of japonica rice using mature embryos and regenerated transgenic plants

Ammar Elakhdar, Masako Fukuda, Takahiko Kubo

Research output: Contribution to journalArticlepeer-review

Abstract

Identification of novel genes and their functions in rice is a critical step to improve economic traits. Agrobacterium tumefaciens-mediated transformation is a proven method in many laboratories and widely adopted for genetic engineering in rice. However, the efficiency of gene transfer by Agrobacterium in rice is low, particularly among japonica and indica varieties. In this protocol, we elucidate a rapid and highly efficient protocol to transform and regenerate transgenic rice plants through important key features of Agrobacterium transformation and standard regeneration media, especially enhancing culture conditions, timing, and growth hormones. With this protocol, transformed plantlets from the embryogenetic callus of the japonica cultivar 'Taichung 65' may be obtained within 90 days. This protocol may be used with other japonica rice varieties.

Original languageEnglish
Article numbere4143
JournalBio-protocol
Volume11
Issue number18
DOIs
Publication statusPublished - Sep 20 2021

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)
  • Plant Science

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