Airborne Monoterpenes Emitted from a Cupressus lusitanica Cell Culture Induce a Signaling Cascade that Produces β-Thujaplicin

Koki Fujita, Ryo Kambe, Ransika De Alwis, Tatsuya Yagi, Yuji Tsutsumi

Research output: Contribution to journalArticle

Abstract

A cell culture of Cupressus lusitanica was used to investigate the reaction of a plant to certain airborne chemicals. Compared with laboratory and field methods using intact plants or tissues, a cell culture is advantageous because it is not affected by environmental factors, and the experiments are easier to reproduce. When exposed to an elicitor, our cell line produces 10 monoterpenes and β-thujaplicin, which is a strong phytoalexin. These monoterpenes are emitted into the vapor phase and are expected to play a role in airborne signaling. In the present study, the cells were exposed to monoterpene vapors, and the volatiles present in the culture flasks were monitored. When the culture cells were exposed to low doses of sabinene, we detected γ-terpinene and p-cymene. After exposure to γ-terpinene, we found p-cymene and terpinolene, whereas p-cymene exposure resulted in terpinolene emission. By contrast, the other seven monoterpenes we investigated did not induce any emissions of other monoterpenes. These results strongly suggest that in C. lusitanica a signaling cascade exists that starts with the emission of sabinene and moves to γ-terpinene, p-cymene, and finally to terpinolene, which accelerates the production of the phytoalexin β-thujaplicin.

Original languageEnglish
Pages (from-to)814-820
Number of pages7
JournalJournal of Chemical Ecology
Volume42
Issue number8
DOIs
Publication statusPublished - Aug 1 2016

Fingerprint

Cupressus lusitanica
Cupressus
Monoterpenes
monoterpene
Cell culture
monoterpenoids
p-cymene
cell culture
terpinolene
Cell Culture Techniques
sabinene
phytoalexins
vapors
Vapors
laboratory method
field method
environmental factor
Cells
cell lines
Tissue

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry

Cite this

Airborne Monoterpenes Emitted from a Cupressus lusitanica Cell Culture Induce a Signaling Cascade that Produces β-Thujaplicin. / Fujita, Koki; Kambe, Ryo; De Alwis, Ransika; Yagi, Tatsuya; Tsutsumi, Yuji.

In: Journal of Chemical Ecology, Vol. 42, No. 8, 01.08.2016, p. 814-820.

Research output: Contribution to journalArticle

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