Abstract
Estrogen has been reported to inhibit apoptosis in vascular endothelial cells. However, its precise mechanism still remains to be elucidated. Here we determined the role of Akt in the anti-apoptotic effect of estrogen. 17β-Estradiol prevented the apoptosis induced by TNF-α in bovine aortic endothelial cells, as evaluated by double staining with fluorescein isothiocyanate-conjugated annexin V and propidium iodide. Introducing a dominant negative mutant of Akt by using a cell-penetrating peptide of Tat protein inhibited the anti-apoptotic effect of estrogen in a concentration-dependent manner, and resulted in the complete inhibition of the anti-apoptotic effect of 17β-estradiol at 1 nM and higher concentrations. The dominant negative mutant without the cell-penetrating peptide and Tat peptide-conjugated protein A had no effect. The intracellular protein transduction was confirmed by immunoblot analysis. Our observations thus provide first direct evidence that Akt plays a central role in the anti-apoptotic effect of estrogen in vascular endothelial cells.
| Original language | English |
|---|---|
| Pages (from-to) | 321-325 |
| Number of pages | 5 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 324 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Nov 5 2004 |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
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Akt plays a central role in the anti-apoptotic effect of estrogen in endothelial cells. / Koga, Miho; Hirano, Katsuya; Hirano, Mayumi; Nishimura, Junji; Nakano, Hitoo; Kanaide, Hideo.
In: Biochemical and Biophysical Research Communications, Vol. 324, No. 1, 05.11.2004, p. 321-325.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Akt plays a central role in the anti-apoptotic effect of estrogen in endothelial cells
AU - Koga, Miho
AU - Hirano, Katsuya
AU - Hirano, Mayumi
AU - Nishimura, Junji
AU - Nakano, Hitoo
AU - Kanaide, Hideo
PY - 2004/11/5
Y1 - 2004/11/5
N2 - Estrogen has been reported to inhibit apoptosis in vascular endothelial cells. However, its precise mechanism still remains to be elucidated. Here we determined the role of Akt in the anti-apoptotic effect of estrogen. 17β-Estradiol prevented the apoptosis induced by TNF-α in bovine aortic endothelial cells, as evaluated by double staining with fluorescein isothiocyanate-conjugated annexin V and propidium iodide. Introducing a dominant negative mutant of Akt by using a cell-penetrating peptide of Tat protein inhibited the anti-apoptotic effect of estrogen in a concentration-dependent manner, and resulted in the complete inhibition of the anti-apoptotic effect of 17β-estradiol at 1 nM and higher concentrations. The dominant negative mutant without the cell-penetrating peptide and Tat peptide-conjugated protein A had no effect. The intracellular protein transduction was confirmed by immunoblot analysis. Our observations thus provide first direct evidence that Akt plays a central role in the anti-apoptotic effect of estrogen in vascular endothelial cells.
AB - Estrogen has been reported to inhibit apoptosis in vascular endothelial cells. However, its precise mechanism still remains to be elucidated. Here we determined the role of Akt in the anti-apoptotic effect of estrogen. 17β-Estradiol prevented the apoptosis induced by TNF-α in bovine aortic endothelial cells, as evaluated by double staining with fluorescein isothiocyanate-conjugated annexin V and propidium iodide. Introducing a dominant negative mutant of Akt by using a cell-penetrating peptide of Tat protein inhibited the anti-apoptotic effect of estrogen in a concentration-dependent manner, and resulted in the complete inhibition of the anti-apoptotic effect of 17β-estradiol at 1 nM and higher concentrations. The dominant negative mutant without the cell-penetrating peptide and Tat peptide-conjugated protein A had no effect. The intracellular protein transduction was confirmed by immunoblot analysis. Our observations thus provide first direct evidence that Akt plays a central role in the anti-apoptotic effect of estrogen in vascular endothelial cells.
UR - http://www.scopus.com/inward/record.url?scp=4744348896&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=4744348896&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2004.09.060
DO - 10.1016/j.bbrc.2004.09.060
M3 - Article
C2 - 15465021
AN - SCOPUS:4744348896
VL - 324
SP - 321
EP - 325
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -