Albumin permeability across endothelial monolayers under long-term shear stress

Susumu Kudo, Masanori Tsuzaka, Mariko Ikeda, Kazuo Tanishita

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Endothelial cells lining the inner surface of blood vessels regulate the exchange of molecules between the blood and the vessel wall. This study investigates the effect of longterm shear stress on macromolecule permeability across endothelial monolayers. In vitro system to measure transendothelial permeability to tetramethylrhodamine conjugated albumin under shear stress was developed. The temporal variation in albumin permeability under 1 Pa and 4 Pa over 48 h was quantified using a fluorescence spectrophotometer. At 4 Pa, albumin permeability did not show a statistical significant change over 48 h. Under shear stress of 1 Pa, the average albumin permeability between 12 and 30 h was 2.5-fold higher than the average between 0 and 6 h. Under shear stress of 1 Pa, the average albumin permeability between 36 and 48 h was 1.8-fold higher than the average between 12 and 30 h. These results show that both the magnitude of, and the length of exposure to, shear stress regulate transendothelial permeability.

Original languageEnglish
Pages (from-to)419-424
Number of pages6
JournalJSME International Journal, Series C: Mechanical Systems, Machine Elements and Manufacturing
Volume48
Issue number4
DOIs
Publication statusPublished - Jun 15 2006
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Mechanical Engineering
  • Industrial and Manufacturing Engineering

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