Albumin permeability across endothelial monolayers under pulsatile shear stress

Susumu Kudo, Minoru Kawarabayashi, Mariko Ikeda, Kazuo Tanishita

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Recent studies suggest that the temporal gradient of shear stress that is generated by blood flow plays an important role in the pathology of arteriosclerosis. We focused on the temporal gradient of shear stress and measured the permeability of albumin under steady or pulsatile shear stress conditions. Porcine aortic endothelial cells were seeded on a membrane filter and subjected to steady or pulsatile shear stress (1 Hz) at 1 Pa for 48 h, and the permeability of albumin was measured over time. The permeability increased gradually under steady flow but increased acutely under pulsatile shear stress. In particular, the maximum permeability of albumin differed under these conditions. The value was 4. 2 × 10-5 cm/s at 18 h under pulsatile shear stress and 2. 8 × 10-5 cm/s at 48 h under steady shear stress. The permeable route of albumin was examined using isoproterenol, which decreases junctional permeability. The increase in albumin permeability with pulsatile shear stress was decreased by isoproterenol. These results suggest that the increased permeability of albumin with pulsatile shear stress was related to trafficking through paracellular junctions. Thus, pulsation may promote a mechanotransduction process that differs from that of steady shear stress, and these pulsation effects likely play an important role in the permeability of macromolecules.

Original languageEnglish
Pages (from-to)38-43
Number of pages6
JournalJournal of Biorheology
Volume26
Issue number1-2
DOIs
Publication statusPublished - Jan 1 2013
Externally publishedYes

Fingerprint

Shear stress
Albumins
Monolayers
Isoproterenol
Endothelial cells
Pathology
Steady flow
Macromolecules
Blood
Membranes

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Albumin permeability across endothelial monolayers under pulsatile shear stress. / Kudo, Susumu; Kawarabayashi, Minoru; Ikeda, Mariko; Tanishita, Kazuo.

In: Journal of Biorheology, Vol. 26, No. 1-2, 01.01.2013, p. 38-43.

Research output: Contribution to journalArticle

Kudo, Susumu ; Kawarabayashi, Minoru ; Ikeda, Mariko ; Tanishita, Kazuo. / Albumin permeability across endothelial monolayers under pulsatile shear stress. In: Journal of Biorheology. 2013 ; Vol. 26, No. 1-2. pp. 38-43.
@article{292f4b5d99fa4e879166f935251cd13b,
title = "Albumin permeability across endothelial monolayers under pulsatile shear stress",
abstract = "Recent studies suggest that the temporal gradient of shear stress that is generated by blood flow plays an important role in the pathology of arteriosclerosis. We focused on the temporal gradient of shear stress and measured the permeability of albumin under steady or pulsatile shear stress conditions. Porcine aortic endothelial cells were seeded on a membrane filter and subjected to steady or pulsatile shear stress (1 Hz) at 1 Pa for 48 h, and the permeability of albumin was measured over time. The permeability increased gradually under steady flow but increased acutely under pulsatile shear stress. In particular, the maximum permeability of albumin differed under these conditions. The value was 4. 2 × 10-5 cm/s at 18 h under pulsatile shear stress and 2. 8 × 10-5 cm/s at 48 h under steady shear stress. The permeable route of albumin was examined using isoproterenol, which decreases junctional permeability. The increase in albumin permeability with pulsatile shear stress was decreased by isoproterenol. These results suggest that the increased permeability of albumin with pulsatile shear stress was related to trafficking through paracellular junctions. Thus, pulsation may promote a mechanotransduction process that differs from that of steady shear stress, and these pulsation effects likely play an important role in the permeability of macromolecules.",
author = "Susumu Kudo and Minoru Kawarabayashi and Mariko Ikeda and Kazuo Tanishita",
year = "2013",
month = "1",
day = "1",
doi = "10.1007/s12573-012-0045-8",
language = "English",
volume = "26",
pages = "38--43",
journal = "Journal of Biorheology",
issn = "1867-0466",
publisher = "Springer Japan",
number = "1-2",

}

TY - JOUR

T1 - Albumin permeability across endothelial monolayers under pulsatile shear stress

AU - Kudo, Susumu

AU - Kawarabayashi, Minoru

AU - Ikeda, Mariko

AU - Tanishita, Kazuo

PY - 2013/1/1

Y1 - 2013/1/1

N2 - Recent studies suggest that the temporal gradient of shear stress that is generated by blood flow plays an important role in the pathology of arteriosclerosis. We focused on the temporal gradient of shear stress and measured the permeability of albumin under steady or pulsatile shear stress conditions. Porcine aortic endothelial cells were seeded on a membrane filter and subjected to steady or pulsatile shear stress (1 Hz) at 1 Pa for 48 h, and the permeability of albumin was measured over time. The permeability increased gradually under steady flow but increased acutely under pulsatile shear stress. In particular, the maximum permeability of albumin differed under these conditions. The value was 4. 2 × 10-5 cm/s at 18 h under pulsatile shear stress and 2. 8 × 10-5 cm/s at 48 h under steady shear stress. The permeable route of albumin was examined using isoproterenol, which decreases junctional permeability. The increase in albumin permeability with pulsatile shear stress was decreased by isoproterenol. These results suggest that the increased permeability of albumin with pulsatile shear stress was related to trafficking through paracellular junctions. Thus, pulsation may promote a mechanotransduction process that differs from that of steady shear stress, and these pulsation effects likely play an important role in the permeability of macromolecules.

AB - Recent studies suggest that the temporal gradient of shear stress that is generated by blood flow plays an important role in the pathology of arteriosclerosis. We focused on the temporal gradient of shear stress and measured the permeability of albumin under steady or pulsatile shear stress conditions. Porcine aortic endothelial cells were seeded on a membrane filter and subjected to steady or pulsatile shear stress (1 Hz) at 1 Pa for 48 h, and the permeability of albumin was measured over time. The permeability increased gradually under steady flow but increased acutely under pulsatile shear stress. In particular, the maximum permeability of albumin differed under these conditions. The value was 4. 2 × 10-5 cm/s at 18 h under pulsatile shear stress and 2. 8 × 10-5 cm/s at 48 h under steady shear stress. The permeable route of albumin was examined using isoproterenol, which decreases junctional permeability. The increase in albumin permeability with pulsatile shear stress was decreased by isoproterenol. These results suggest that the increased permeability of albumin with pulsatile shear stress was related to trafficking through paracellular junctions. Thus, pulsation may promote a mechanotransduction process that differs from that of steady shear stress, and these pulsation effects likely play an important role in the permeability of macromolecules.

UR - http://www.scopus.com/inward/record.url?scp=84875369333&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84875369333&partnerID=8YFLogxK

U2 - 10.1007/s12573-012-0045-8

DO - 10.1007/s12573-012-0045-8

M3 - Article

AN - SCOPUS:84875369333

VL - 26

SP - 38

EP - 43

JO - Journal of Biorheology

JF - Journal of Biorheology

SN - 1867-0466

IS - 1-2

ER -