Albumin uptake into endothelial cells in separated flow

Susumu Kudo, Ryuhei Yamaguchi, Masashi Sato, Kotaro Oka, Kazuo Tanishita

Research output: Contribution to journalArticle

Abstract

The purpose of this study is to reveal the albumin uptake into endothelial cells in the separated flow area. After 24 hr of exposure to flow induced in a back step flow channel, the endothelial cells were incubated in 37 °C for 60 minutes in PBS containing tetramethylrhodamine isothiocyanate conjugated albumin (TRITC-albumin). Thereafter, the cell morphology and the albumin uptake were observed by a confocal laser scanning microscope (CLSM). In a low shear stress area (stagnant and reattachment areas), the cells aligned randomly. In a high shear stress area (reversal and fully developed areas), the cells were elongated and aligned to flow direction. In low-shear-stress and high-shear-stress gradient areas (reattachment areas), the amount of albumin uptake into the cells was the largest in all areas. These data indicate that shear stress and shear stress gradient affect the endothelial cell morphology and the albumin uptake into endothelial cells.

Original languageEnglish
Pages (from-to)83-84
Number of pages2
JournalAmerican Society of Mechanical Engineers, Bioengineering Division (Publication) BED
Volume48
Publication statusPublished - Dec 1 2000
Externally publishedYes

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Endothelial cells
Shear stress
Channel flow
Microscopes
Scanning
Lasers

All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Albumin uptake into endothelial cells in separated flow. / Kudo, Susumu; Yamaguchi, Ryuhei; Sato, Masashi; Oka, Kotaro; Tanishita, Kazuo.

In: American Society of Mechanical Engineers, Bioengineering Division (Publication) BED, Vol. 48, 01.12.2000, p. 83-84.

Research output: Contribution to journalArticle

Kudo, Susumu ; Yamaguchi, Ryuhei ; Sato, Masashi ; Oka, Kotaro ; Tanishita, Kazuo. / Albumin uptake into endothelial cells in separated flow. In: American Society of Mechanical Engineers, Bioengineering Division (Publication) BED. 2000 ; Vol. 48. pp. 83-84.
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