ALCAM (CD166): Its role in hematopoietic and endothelial development

Osamu Ohneda, Kinuko Ohneda, Fumio Arai, James Lee, Takeshi Miyamoto, Yoshimi Fukushima, Donald Dowbenko, Laurence A. Lasky, Toshio Suda

Research output: Contribution to journalArticle

111 Citations (Scopus)

Abstract

A critical role for the endothelium of yolk sac and dorsal aorta has been shown in embryonic hematopoiesis. A stromal cell line derived from yolk sac, YSCL-72, has been chosen to search for a novel molecule associated with embryonic hematopoiesis. Analysis between YSCL-72 and an adult aorta-derived endothelial cell line, EOMA, demonstrated that activated leukocyte cell adhesion molecule (ALCAM, or CD166) was specifically expressed in YSCL-72 but not in EOMA. Immunohistochemical study showed that ALCAM was expressed in the endothelium of yolk sac and dorsal aorta but not in adult aorta. ALCAM-transfected EOMA cells supported development of hematopoietic progenitor cells compared with vector-transfected EOMA cells, suggesting that ALCAM appeared to be crucial for hematopoiesis. In addition, ALCAM was found to be involved in capillary tube formation and hemangioblast differentiation. Taken together with these findings, ALCAM is highly associated not only with embryonic hematopoiesis but also vasculoangiogenesis.

Original languageEnglish
Pages (from-to)2134-2142
Number of pages9
JournalBlood
Volume98
Issue number7
DOIs
Publication statusPublished - Oct 1 2001
Externally publishedYes

Fingerprint

Activated-Leukocyte Cell Adhesion Molecule
Hematopoiesis
Yolk Sac
Aorta
Endothelium
Hemangioblasts
Cell Line
Capillary tubes
Endothelial cells
Stromal Cells
Hematopoietic Stem Cells
Endothelial Cells
Cells
Molecules

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Ohneda, O., Ohneda, K., Arai, F., Lee, J., Miyamoto, T., Fukushima, Y., ... Suda, T. (2001). ALCAM (CD166): Its role in hematopoietic and endothelial development. Blood, 98(7), 2134-2142. https://doi.org/10.1182/blood.V98.7.2134

ALCAM (CD166) : Its role in hematopoietic and endothelial development. / Ohneda, Osamu; Ohneda, Kinuko; Arai, Fumio; Lee, James; Miyamoto, Takeshi; Fukushima, Yoshimi; Dowbenko, Donald; Lasky, Laurence A.; Suda, Toshio.

In: Blood, Vol. 98, No. 7, 01.10.2001, p. 2134-2142.

Research output: Contribution to journalArticle

Ohneda, O, Ohneda, K, Arai, F, Lee, J, Miyamoto, T, Fukushima, Y, Dowbenko, D, Lasky, LA & Suda, T 2001, 'ALCAM (CD166): Its role in hematopoietic and endothelial development', Blood, vol. 98, no. 7, pp. 2134-2142. https://doi.org/10.1182/blood.V98.7.2134
Ohneda O, Ohneda K, Arai F, Lee J, Miyamoto T, Fukushima Y et al. ALCAM (CD166): Its role in hematopoietic and endothelial development. Blood. 2001 Oct 1;98(7):2134-2142. https://doi.org/10.1182/blood.V98.7.2134
Ohneda, Osamu ; Ohneda, Kinuko ; Arai, Fumio ; Lee, James ; Miyamoto, Takeshi ; Fukushima, Yoshimi ; Dowbenko, Donald ; Lasky, Laurence A. ; Suda, Toshio. / ALCAM (CD166) : Its role in hematopoietic and endothelial development. In: Blood. 2001 ; Vol. 98, No. 7. pp. 2134-2142.
@article{63a06f65b2b14c94b2b832590b120725,
title = "ALCAM (CD166): Its role in hematopoietic and endothelial development",
abstract = "A critical role for the endothelium of yolk sac and dorsal aorta has been shown in embryonic hematopoiesis. A stromal cell line derived from yolk sac, YSCL-72, has been chosen to search for a novel molecule associated with embryonic hematopoiesis. Analysis between YSCL-72 and an adult aorta-derived endothelial cell line, EOMA, demonstrated that activated leukocyte cell adhesion molecule (ALCAM, or CD166) was specifically expressed in YSCL-72 but not in EOMA. Immunohistochemical study showed that ALCAM was expressed in the endothelium of yolk sac and dorsal aorta but not in adult aorta. ALCAM-transfected EOMA cells supported development of hematopoietic progenitor cells compared with vector-transfected EOMA cells, suggesting that ALCAM appeared to be crucial for hematopoiesis. In addition, ALCAM was found to be involved in capillary tube formation and hemangioblast differentiation. Taken together with these findings, ALCAM is highly associated not only with embryonic hematopoiesis but also vasculoangiogenesis.",
author = "Osamu Ohneda and Kinuko Ohneda and Fumio Arai and James Lee and Takeshi Miyamoto and Yoshimi Fukushima and Donald Dowbenko and Lasky, {Laurence A.} and Toshio Suda",
year = "2001",
month = "10",
day = "1",
doi = "10.1182/blood.V98.7.2134",
language = "English",
volume = "98",
pages = "2134--2142",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "7",

}

TY - JOUR

T1 - ALCAM (CD166)

T2 - Its role in hematopoietic and endothelial development

AU - Ohneda, Osamu

AU - Ohneda, Kinuko

AU - Arai, Fumio

AU - Lee, James

AU - Miyamoto, Takeshi

AU - Fukushima, Yoshimi

AU - Dowbenko, Donald

AU - Lasky, Laurence A.

AU - Suda, Toshio

PY - 2001/10/1

Y1 - 2001/10/1

N2 - A critical role for the endothelium of yolk sac and dorsal aorta has been shown in embryonic hematopoiesis. A stromal cell line derived from yolk sac, YSCL-72, has been chosen to search for a novel molecule associated with embryonic hematopoiesis. Analysis between YSCL-72 and an adult aorta-derived endothelial cell line, EOMA, demonstrated that activated leukocyte cell adhesion molecule (ALCAM, or CD166) was specifically expressed in YSCL-72 but not in EOMA. Immunohistochemical study showed that ALCAM was expressed in the endothelium of yolk sac and dorsal aorta but not in adult aorta. ALCAM-transfected EOMA cells supported development of hematopoietic progenitor cells compared with vector-transfected EOMA cells, suggesting that ALCAM appeared to be crucial for hematopoiesis. In addition, ALCAM was found to be involved in capillary tube formation and hemangioblast differentiation. Taken together with these findings, ALCAM is highly associated not only with embryonic hematopoiesis but also vasculoangiogenesis.

AB - A critical role for the endothelium of yolk sac and dorsal aorta has been shown in embryonic hematopoiesis. A stromal cell line derived from yolk sac, YSCL-72, has been chosen to search for a novel molecule associated with embryonic hematopoiesis. Analysis between YSCL-72 and an adult aorta-derived endothelial cell line, EOMA, demonstrated that activated leukocyte cell adhesion molecule (ALCAM, or CD166) was specifically expressed in YSCL-72 but not in EOMA. Immunohistochemical study showed that ALCAM was expressed in the endothelium of yolk sac and dorsal aorta but not in adult aorta. ALCAM-transfected EOMA cells supported development of hematopoietic progenitor cells compared with vector-transfected EOMA cells, suggesting that ALCAM appeared to be crucial for hematopoiesis. In addition, ALCAM was found to be involved in capillary tube formation and hemangioblast differentiation. Taken together with these findings, ALCAM is highly associated not only with embryonic hematopoiesis but also vasculoangiogenesis.

UR - http://www.scopus.com/inward/record.url?scp=0035496923&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035496923&partnerID=8YFLogxK

U2 - 10.1182/blood.V98.7.2134

DO - 10.1182/blood.V98.7.2134

M3 - Article

C2 - 11568000

AN - SCOPUS:0035496923

VL - 98

SP - 2134

EP - 2142

JO - Blood

JF - Blood

SN - 0006-4971

IS - 7

ER -