Aldehyde dehydrogenase (ALDH) 2 associates with oxidation of methoxyacetaldehyde; in vitro analysis with liver subcellular fraction derived from human and Aldh2 gene targeting mouse

Kyoko Kitagawa, Toshihiro Kawamoto, Naoki Kunugita, Tadasuke Tsukiyama, Kohji Okamoto, Akira Yoshida, Keiko Nakayama, Keiichi Nakayama

Research output: Contribution to journalArticle

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Abstract

A principal pathway of 2-methoxyethanol (ME) metabolism is to the toxic oxidative product, methoxyacetaldehyde (MALD). To assess the role of aldehyde dehydrogenase (ALDH) in MALD metabolism, in vitro MALD oxidation was examined with liver subcellular fractions from Japanese subjects who carried three different ALDH2 genotypes and Aldh2 knockout mice, which were generated in this study. The activity was distributed in mitochondrial fractions of ALDH2*1/*1 and wild type (Aldh2+/+) mice but not ALDH2*1/*2, *2/*2 subjects or Aldh2 homozygous mutant (Aldh2-/-) mice. These data suggest that ALDH2 is a key enzyme for MALD oxidation and ME susceptibility may be influenced by the ALDH2 genotype. Copyright (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)306-311
Number of pages6
JournalFEBS Letters
Volume476
Issue number3
DOIs
Publication statusPublished - Jul 7 2000

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Aldehyde Dehydrogenase
Subcellular Fractions
Gene Targeting
Liver
Genes
Oxidation
Metabolism
Genotype
Poisons
Knockout Mice
2-methoxyacetaldehyde
In Vitro Techniques
Enzymes
methyl cellosolve

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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Aldehyde dehydrogenase (ALDH) 2 associates with oxidation of methoxyacetaldehyde; in vitro analysis with liver subcellular fraction derived from human and Aldh2 gene targeting mouse. / Kitagawa, Kyoko; Kawamoto, Toshihiro; Kunugita, Naoki; Tsukiyama, Tadasuke; Okamoto, Kohji; Yoshida, Akira; Nakayama, Keiko; Nakayama, Keiichi.

In: FEBS Letters, Vol. 476, No. 3, 07.07.2000, p. 306-311.

Research output: Contribution to journalArticle

Kitagawa, Kyoko ; Kawamoto, Toshihiro ; Kunugita, Naoki ; Tsukiyama, Tadasuke ; Okamoto, Kohji ; Yoshida, Akira ; Nakayama, Keiko ; Nakayama, Keiichi. / Aldehyde dehydrogenase (ALDH) 2 associates with oxidation of methoxyacetaldehyde; in vitro analysis with liver subcellular fraction derived from human and Aldh2 gene targeting mouse. In: FEBS Letters. 2000 ; Vol. 476, No. 3. pp. 306-311.
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