Alginate immobilization of Morus alba L. cell suspension cultures improved the accumulation and secretion of stilbenoids

Chadathorn Inyai, Panitch Boonsnongcheep, Jukrapun Komaikul, Boonchoo Sritularak, Hiroyuki Tanaka, Waraporn Putalun

Research output: Contribution to journalArticle

Abstract

Morus alba L. (Moraceae) has been used in traditional medicine for the treatment of several illnesses. Recent research also revealed several pharmacological activities from many groups of secondary metabolites, including the stilbenoids mulberroside A, oxyresveratrol, and resveratrol, which are promising compounds for cosmetic and herbal supplement products. In our previous study, cell cultures of M. alba showed high productivity of these compounds. In this study, we attempted to develop immobilized cell cultures of M. alba and to test the effect of elicitors and precursors on the production of stilbenoids. The immobilization of the M. alba cells significantly promoted the secretion of mulberroside A into the extracellular matrix and culture media to 60%, while enhancing the level of oxyresveratrol and resveratrol by 12- and 27-fold, respectively. The elicitation of immobilized cells with a combination of 50 µM methyl jasmonate and 0.5 mg/mL yeast extract for 24 h promoted a twofold increase in the production of all three stilbenoids. Furthermore, the addition of 0.05 mM l-phenylalanine, 0.03 mM l-tyrosine, or a combination resulted in the enhancement of mulberroside A production for up to twofold. The addition of l-tyrosine significantly enhanced the production of oxyresveratrol and resveratrol. This is the first report of stilbenoid production using immobilized cell cultures of M. alba. The cultures have benefits over normal cell suspension cultures by promoting the secretion of mulberroside A and enhancing the levels of oxyresveratrol and resveratrol. Thus, it could be a candidate method for the production of these stilbenoids.

LanguageEnglish
Pages131-141
Number of pages11
JournalBioprocess and Biosystems Engineering
Volume42
Issue number1
DOIs
Publication statusPublished - Jan 30 2019

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Morus
Alginate
Resveratrol
Immobilized Cells
Immobilization
Suspensions
Cell Culture Techniques
Cell culture
Tyrosine
Moraceae
Traditional Medicine
Phenylalanine
Cosmetics
Extracellular Matrix
Culture Media
Yeasts
Pharmacology
Metabolites
Yeast
Medicine

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering

Cite this

Alginate immobilization of Morus alba L. cell suspension cultures improved the accumulation and secretion of stilbenoids. / Inyai, Chadathorn; Boonsnongcheep, Panitch; Komaikul, Jukrapun; Sritularak, Boonchoo; Tanaka, Hiroyuki; Putalun, Waraporn.

In: Bioprocess and Biosystems Engineering, Vol. 42, No. 1, 30.01.2019, p. 131-141.

Research output: Contribution to journalArticle

Inyai, Chadathorn ; Boonsnongcheep, Panitch ; Komaikul, Jukrapun ; Sritularak, Boonchoo ; Tanaka, Hiroyuki ; Putalun, Waraporn. / Alginate immobilization of Morus alba L. cell suspension cultures improved the accumulation and secretion of stilbenoids. In: Bioprocess and Biosystems Engineering. 2019 ; Vol. 42, No. 1. pp. 131-141.
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