Alkali-soluble pectin suppresses IgE production in human myeloma cell line in vitro

Akira Iwamoto, Yuichi Inoue, Hirofumi Tachibana, Hiroharu Kawahara

Research output: Contribution to journalArticle

Abstract

We found that strawberry (Fragaria x ananassa) extract has an IgE production suppressive activity and its oral administration improved skin manifestation in atopic dermatitis model mice. In present study, we identified an active substance using the IgE-producing human myeloma cell line U266. Gel filtration experiment indicated that the IgE suppressor was more than 6 kDa in molecular size. In addition, its pectinase treatment inhibited the activity, suggesting that the active substance in strawberry extract is pectin. Among solutions of water-(WP), hexametaphosphate-(HXP), acid-(HP) and alkali soluble pectin (OHP) extracted from strawberry, only OHP suppressed IgE production, and their suppressive activity was cancelled by pectinase treatment. In addition, OHP extracted from apple also inhibited IgE production. Furthermore, OHP also suppressed IgE production and did not affect IgG and IgM production in human peripheral blood mononuclear cells in an in vitro immunization condition. From these results, we concluded that OHP was an IgE suppressor in strawberry extract.

Original languageEnglish
Pages (from-to)573-581
Number of pages9
JournalCytotechnology
Volume71
Issue number2
DOIs
Publication statusPublished - Apr 15 2019

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Alkalies
Immunoglobulin E
Fragaria
Cells
Cell Line
Polygalacturonase
Immunization
Skin Manifestations
Skin
Blood
Gels
Malus
Atopic Dermatitis
pectin
In Vitro Techniques
Gel Chromatography
Immunoglobulin M
Oral Administration
Blood Cells
Acids

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Clinical Biochemistry
  • Cell Biology

Cite this

Alkali-soluble pectin suppresses IgE production in human myeloma cell line in vitro. / Iwamoto, Akira; Inoue, Yuichi; Tachibana, Hirofumi; Kawahara, Hiroharu.

In: Cytotechnology, Vol. 71, No. 2, 15.04.2019, p. 573-581.

Research output: Contribution to journalArticle

Iwamoto, Akira ; Inoue, Yuichi ; Tachibana, Hirofumi ; Kawahara, Hiroharu. / Alkali-soluble pectin suppresses IgE production in human myeloma cell line in vitro. In: Cytotechnology. 2019 ; Vol. 71, No. 2. pp. 573-581.
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