Abstract
Despite the expanding use of flow cytometry, its detection limit is not satisfactory for many antigen proteins with low copy numbers. Herein, we describe an alkaline phosphatase (AP)-based technique to amplify the fluorescence signal for cell staining applications. We designed a fluorescent substrate that acquires membrane permeability upon dephosphorylation by AP. By using the substrate, the fluorescence signal of cells in flow cytometry could be successfully amplified to give a much stronger signal than the cells labeled using a conventional fluorophore-modified antibody.
| Original language | English |
|---|---|
| Pages (from-to) | 1059-1062 |
| Number of pages | 4 |
| Journal | Analytical chemistry |
| Volume | 90 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jan 16 2018 |
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry
Cite this
Alkaline Phosphatase-Catalyzed Amplification of a Fluorescence Signal for Flow Cytometry. / Nobori, Takanobu; Tosaka, Kenta; Kawamura, Akira; Joichi, Taisei; Kamino, Kenta; Kishimura, Akihiro; Baba, Eishi; Mori, Takeshi; Katayama, Yoshiki.
In: Analytical chemistry, Vol. 90, No. 2, 16.01.2018, p. 1059-1062.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Alkaline Phosphatase-Catalyzed Amplification of a Fluorescence Signal for Flow Cytometry
AU - Nobori, Takanobu
AU - Tosaka, Kenta
AU - Kawamura, Akira
AU - Joichi, Taisei
AU - Kamino, Kenta
AU - Kishimura, Akihiro
AU - Baba, Eishi
AU - Mori, Takeshi
AU - Katayama, Yoshiki
PY - 2018/1/16
Y1 - 2018/1/16
N2 - Despite the expanding use of flow cytometry, its detection limit is not satisfactory for many antigen proteins with low copy numbers. Herein, we describe an alkaline phosphatase (AP)-based technique to amplify the fluorescence signal for cell staining applications. We designed a fluorescent substrate that acquires membrane permeability upon dephosphorylation by AP. By using the substrate, the fluorescence signal of cells in flow cytometry could be successfully amplified to give a much stronger signal than the cells labeled using a conventional fluorophore-modified antibody.
AB - Despite the expanding use of flow cytometry, its detection limit is not satisfactory for many antigen proteins with low copy numbers. Herein, we describe an alkaline phosphatase (AP)-based technique to amplify the fluorescence signal for cell staining applications. We designed a fluorescent substrate that acquires membrane permeability upon dephosphorylation by AP. By using the substrate, the fluorescence signal of cells in flow cytometry could be successfully amplified to give a much stronger signal than the cells labeled using a conventional fluorophore-modified antibody.
UR - http://www.scopus.com/inward/record.url?scp=85040672934&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85040672934&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.7b03893
DO - 10.1021/acs.analchem.7b03893
M3 - Article
C2 - 29260552
AN - SCOPUS:85040672934
VL - 90
SP - 1059
EP - 1062
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 2
ER -