Alkaline Phosphatase-Catalyzed Amplification of a Fluorescence Signal for Flow Cytometry

Takanobu Nobori, Kenta Tosaka, Akira Kawamura, Taisei Joichi, Kenta Kamino, Akihiro Kishimura, Eishi Baba, Takeshi Mori, Yoshiki Katayama

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


Despite the expanding use of flow cytometry, its detection limit is not satisfactory for many antigen proteins with low copy numbers. Herein, we describe an alkaline phosphatase (AP)-based technique to amplify the fluorescence signal for cell staining applications. We designed a fluorescent substrate that acquires membrane permeability upon dephosphorylation by AP. By using the substrate, the fluorescence signal of cells in flow cytometry could be successfully amplified to give a much stronger signal than the cells labeled using a conventional fluorophore-modified antibody.

Original languageEnglish
Pages (from-to)1059-1062
Number of pages4
JournalAnalytical chemistry
Issue number2
Publication statusPublished - Jan 16 2018

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Fingerprint Dive into the research topics of 'Alkaline Phosphatase-Catalyzed Amplification of a Fluorescence Signal for Flow Cytometry'. Together they form a unique fingerprint.

Cite this