Alkaline Phosphatase-Catalyzed Amplification of a Fluorescence Signal for Flow Cytometry

Takanobu Nobori, Kenta Tosaka, Akira Kawamura, Taisei Joichi, Kenta Kamino, Akihiro Kishimura, Eishi Baba, Takeshi Mori, Yoshiki Katayama

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Despite the expanding use of flow cytometry, its detection limit is not satisfactory for many antigen proteins with low copy numbers. Herein, we describe an alkaline phosphatase (AP)-based technique to amplify the fluorescence signal for cell staining applications. We designed a fluorescent substrate that acquires membrane permeability upon dephosphorylation by AP. By using the substrate, the fluorescence signal of cells in flow cytometry could be successfully amplified to give a much stronger signal than the cells labeled using a conventional fluorophore-modified antibody.

Original languageEnglish
Pages (from-to)1059-1062
Number of pages4
JournalAnalytical chemistry
Volume90
Issue number2
DOIs
Publication statusPublished - Jan 16 2018

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Flow cytometry
Amplification
Alkaline Phosphatase
Fluorescence
Fluorophores
Substrates
Membranes
Antigens
Antibodies
Proteins

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Alkaline Phosphatase-Catalyzed Amplification of a Fluorescence Signal for Flow Cytometry. / Nobori, Takanobu; Tosaka, Kenta; Kawamura, Akira; Joichi, Taisei; Kamino, Kenta; Kishimura, Akihiro; Baba, Eishi; Mori, Takeshi; Katayama, Yoshiki.

In: Analytical chemistry, Vol. 90, No. 2, 16.01.2018, p. 1059-1062.

Research output: Contribution to journalArticle

Nobori, Takanobu ; Tosaka, Kenta ; Kawamura, Akira ; Joichi, Taisei ; Kamino, Kenta ; Kishimura, Akihiro ; Baba, Eishi ; Mori, Takeshi ; Katayama, Yoshiki. / Alkaline Phosphatase-Catalyzed Amplification of a Fluorescence Signal for Flow Cytometry. In: Analytical chemistry. 2018 ; Vol. 90, No. 2. pp. 1059-1062.
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