Allelopathic activity of buckwheat: Isolation and characterization of phenolics

Zahida Iqbal, Syuntaro Hiradate, Akio Noda, Sei Ichi Isojima, Yoshiharu Fujii

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Laboratory and field experiments were conducted to assess the allelopathic potential of buckwheat. In the field, buckwheat demonstrated strong inhibitory activity by suppressing weeds. In laboratory studies, aqueous and organic solvent extracts of the aerial parts of common buckwheat inhibited the root and shoot growth of lettuce seedlings. The chloroform and ethyl acetate extracts showed maximum activity, and plants grown in the presence of the ethyl acetate extract showed severe root browning. The allelopathic constituents of the ethyl acetate phase were isolated and identified as gallic acid and (+)-catechin by nuclear magnetic resonance spectroscopy. Gallic acid and (+)-catechin were present in the upper part of buckwheat at concentrations of 0.02 and 0.01%, of fresh weight, respectively. Gallic acid was found to be selectively and strongly inhibitory to root and shoot growth of tested plants at 100 and 10 μg ml-1. (+)-Catechin, however, inhibited plant growth to a lesser extent. These results suggest that buckwheat may have allelopathic potential and that when used as a ground cover crop or green manure may produce inhibitors, which could suppress weeds.

Original languageEnglish
Pages (from-to)657-662
Number of pages6
JournalWeed Science
Volume51
Issue number5
DOIs
Publication statusPublished - Dec 1 2003
Externally publishedYes

Fingerprint

allelopathy
buckwheat
gallic acid
catechin
extracts
weeds
plant growth
shoots
green manures
cover crops
chloroform
lettuce
aerial parts
nuclear magnetic resonance spectroscopy
seedlings
ethyl acetate
water

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

Cite this

Allelopathic activity of buckwheat : Isolation and characterization of phenolics. / Iqbal, Zahida; Hiradate, Syuntaro; Noda, Akio; Isojima, Sei Ichi; Fujii, Yoshiharu.

In: Weed Science, Vol. 51, No. 5, 01.12.2003, p. 657-662.

Research output: Contribution to journalArticle

Iqbal, Zahida ; Hiradate, Syuntaro ; Noda, Akio ; Isojima, Sei Ichi ; Fujii, Yoshiharu. / Allelopathic activity of buckwheat : Isolation and characterization of phenolics. In: Weed Science. 2003 ; Vol. 51, No. 5. pp. 657-662.
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