Allocation dependence of nano-pillars for DNA electrophoresis separation

Ryo Ogawa, Akio Oki, Shingi Hashioka, Noritada Kaji, Yoshinobu Baba, Yasuhiro Horiike

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Different allocations of nano-pillars were investigated for DNA electrophoresis separation. The square allocation shows that DNA was extended and moved straight without disturbance of pillars, but DNA entangled around pillars in the case of the tilt allocation. The tilt allocation allowed us to separate DNA of 10 kbp and 38 kbp by their sizes, whereas square allocation demonstrated one broad peak alone. The separation in the tilt nano-pillars pattern is considered to result from the sieving effect for different sizes of DNA. These results suggest that any disturbance is necessary for electrophoretic separation of small size DNA.

Original languageEnglish
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference
Subtitle of host publication9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherTransducer Research Foundation
Pages1012-1014
Number of pages3
ISBN (Print)0974361119, 9780974361116
Publication statusPublished - Jan 1 2005
Externally publishedYes
Event9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States
Duration: Oct 9 2005Oct 13 2005

Publication series

NameMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Volume1

Other

Other9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
CountryUnited States
CityBoston, MA
Period10/9/0510/13/05

Fingerprint

Electrophoresis
DNA

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Ogawa, R., Oki, A., Hashioka, S., Kaji, N., Baba, Y., & Horiike, Y. (2005). Allocation dependence of nano-pillars for DNA electrophoresis separation. In Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1012-1014). (Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences; Vol. 1). Transducer Research Foundation.

Allocation dependence of nano-pillars for DNA electrophoresis separation. / Ogawa, Ryo; Oki, Akio; Hashioka, Shingi; Kaji, Noritada; Baba, Yoshinobu; Horiike, Yasuhiro.

Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Transducer Research Foundation, 2005. p. 1012-1014 (Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences; Vol. 1).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ogawa, R, Oki, A, Hashioka, S, Kaji, N, Baba, Y & Horiike, Y 2005, Allocation dependence of nano-pillars for DNA electrophoresis separation. in Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, vol. 1, Transducer Research Foundation, pp. 1012-1014, 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005, Boston, MA, United States, 10/9/05.
Ogawa R, Oki A, Hashioka S, Kaji N, Baba Y, Horiike Y. Allocation dependence of nano-pillars for DNA electrophoresis separation. In Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Transducer Research Foundation. 2005. p. 1012-1014. (Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences).
Ogawa, Ryo ; Oki, Akio ; Hashioka, Shingi ; Kaji, Noritada ; Baba, Yoshinobu ; Horiike, Yasuhiro. / Allocation dependence of nano-pillars for DNA electrophoresis separation. Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Transducer Research Foundation, 2005. pp. 1012-1014 (Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences).
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