TY - JOUR
T1 - Allograft inflammatory factor-1 augments macrophage phagocytotic activity and accelerates the progression of atherosclerosis in ApoE-/- mice
AU - Mishima, Tetsuya
AU - Iwabuchi, Kazuya
AU - Fujii, Satoshi
AU - Tanaka, Shin Ya
AU - Ogura, Hisako
AU - Watano-Miyata, Keiko
AU - Ishimori, Naoki
AU - Andoh, Yasuhiro
AU - Nakai, Yukihito
AU - Iwabuchi, Chikako
AU - Ato, Manabu
AU - Kitabatake, Akira
AU - Tsutsui, Hiroyuki
AU - Onoé, Kazunori
PY - 2008/2
Y1 - 2008/2
N2 - Allograft inflammatory factor (AIF)-1, originally cloned from a rat heart allograft under chronic rejection, is induced in various inflammatory conditions including atherosclerosis. Using mouse AIF-1 transfected macrophages and AIF-1 transgenic (AIF-1Tg) mice, we analyzed the influence of AIF-1 overexpression on macrophage phagocytosis and the development of atherosclerosis. The AIF-1 transfectants showed significantly increased phagocytosis of latex beads and E. coli BioParticles as well as incorporation of acetylated low-density lipoprotein (LDL) compared to those of vector controls. Concordant results were obtained with elicited peritoneal exudate cells from AIF-1Tg mice. When AIF- 1Tg mice were crossbred with apolipoprotein E knockout mice (ApoE-/-), these AIF-1TgApoE-/- mice developed significantly increased atherosclerotic lesions compared to ApoE-/- mice. These results suggest that enhanced AIF-1 expression leads to augmented incorporation of degenerated LDL by macrophages and promotes development of astherosclerotic vasculopathy.
AB - Allograft inflammatory factor (AIF)-1, originally cloned from a rat heart allograft under chronic rejection, is induced in various inflammatory conditions including atherosclerosis. Using mouse AIF-1 transfected macrophages and AIF-1 transgenic (AIF-1Tg) mice, we analyzed the influence of AIF-1 overexpression on macrophage phagocytosis and the development of atherosclerosis. The AIF-1 transfectants showed significantly increased phagocytosis of latex beads and E. coli BioParticles as well as incorporation of acetylated low-density lipoprotein (LDL) compared to those of vector controls. Concordant results were obtained with elicited peritoneal exudate cells from AIF-1Tg mice. When AIF- 1Tg mice were crossbred with apolipoprotein E knockout mice (ApoE-/-), these AIF-1TgApoE-/- mice developed significantly increased atherosclerotic lesions compared to ApoE-/- mice. These results suggest that enhanced AIF-1 expression leads to augmented incorporation of degenerated LDL by macrophages and promotes development of astherosclerotic vasculopathy.
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U2 - 10.3892/ijmm.21.2.181
DO - 10.3892/ijmm.21.2.181
M3 - Article
C2 - 18204784
AN - SCOPUS:38949135951
SN - 1107-3756
VL - 21
SP - 181
EP - 187
JO - International Journal of Molecular Medicine
JF - International Journal of Molecular Medicine
IS - 2
ER -