Allograft inflammatory factor-1 augments macrophage phagocytotic activity and accelerates the progression of atherosclerosis in ApoE-/- mice

Tetsuya Mishima, Kazuya Iwabuchi, Satoshi Fujii, Shin Ya Tanaka, Hisako Ogura, Keiko Watano-Miyata, Naoki Ishimori, Yasuhiro Andoh, Yukihito Nakai, Chikako Iwabuchi, Manabu Ato, Akira Kitabatake, Hiroyuki Tsutsui, Kazunori Onoé

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

Allograft inflammatory factor (AIF)-1, originally cloned from a rat heart allograft under chronic rejection, is induced in various inflammatory conditions including atherosclerosis. Using mouse AIF-1 transfected macrophages and AIF-1 transgenic (AIF-1Tg) mice, we analyzed the influence of AIF-1 overexpression on macrophage phagocytosis and the development of atherosclerosis. The AIF-1 transfectants showed significantly increased phagocytosis of latex beads and E. coli BioParticles as well as incorporation of acetylated low-density lipoprotein (LDL) compared to those of vector controls. Concordant results were obtained with elicited peritoneal exudate cells from AIF-1Tg mice. When AIF- 1Tg mice were crossbred with apolipoprotein E knockout mice (ApoE-/-), these AIF-1TgApoE-/- mice developed significantly increased atherosclerotic lesions compared to ApoE-/- mice. These results suggest that enhanced AIF-1 expression leads to augmented incorporation of degenerated LDL by macrophages and promotes development of astherosclerotic vasculopathy.

Original languageEnglish
Pages (from-to)181-187
Number of pages7
JournalInternational journal of molecular medicine
Volume21
Issue number2
DOIs
Publication statusPublished - Feb 2008
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics

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