Allograft inflammatory factor 1 is a regulator of transcytosis in M cells

Sari Kishikawa, Shintaro S.S. Sato, Satoshi Kaneto, Shigeo Uchino, Shinichi Kohsaka, Seiji Nakamura, Hiroshi Kiyono

Research output: Contribution to journalArticle

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Abstract

M cells in follicle-associated epithelium (FAE) are specialized antigen-sampling cells that take up intestinal luminal antigens. Transcription factor Spi-B regulates M-cell maturation, but the molecules that promote transcytosis within M cells are not fully identified. Here we show that mouse allograft inflammatory factor 1 (Aif1) is expressed by M cells and contributes to M-cell transcytosis. FAE in Aif1 -/- mice has suppressed uptake of particles and commensal bacteria, compared with wild-type mice. Translocation of Yersinia enterocolitica, but not of Salmonella enterica serovar Typhimurium, leading to the generation of antigen-specific IgA antibodies, is also diminished in Aif1-deficient mice. Although β1 integrin, which acts as a receptor for Y. enterocolitica via invasin protein, is expressed on the apical surface membranes of M cells, its active form is rarely found in Aif1 -/- mice. These findings show that Aif1 is important for bacterial and particle transcytosis in M cells.

Original languageEnglish
Article number14509
JournalNature communications
Volume8
DOIs
Publication statusPublished - Feb 22 2017

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Transcytosis
regulators
Allografts
mice
cells
antigens
Antigens
Yersinia enterocolitica
epithelium
Salmonella
Epithelium
Integrins
Immunoglobulin A
Salmonella enterica
salmonella
Bacteria
Transcription Factors
Sampling
Membranes
antibodies

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Kishikawa, S., Sato, S. S. S., Kaneto, S., Uchino, S., Kohsaka, S., Nakamura, S., & Kiyono, H. (2017). Allograft inflammatory factor 1 is a regulator of transcytosis in M cells. Nature communications, 8, [14509]. https://doi.org/10.1038/ncomms14509

Allograft inflammatory factor 1 is a regulator of transcytosis in M cells. / Kishikawa, Sari; Sato, Shintaro S.S.; Kaneto, Satoshi; Uchino, Shigeo; Kohsaka, Shinichi; Nakamura, Seiji; Kiyono, Hiroshi.

In: Nature communications, Vol. 8, 14509, 22.02.2017.

Research output: Contribution to journalArticle

Kishikawa, S, Sato, SSS, Kaneto, S, Uchino, S, Kohsaka, S, Nakamura, S & Kiyono, H 2017, 'Allograft inflammatory factor 1 is a regulator of transcytosis in M cells', Nature communications, vol. 8, 14509. https://doi.org/10.1038/ncomms14509
Kishikawa, Sari ; Sato, Shintaro S.S. ; Kaneto, Satoshi ; Uchino, Shigeo ; Kohsaka, Shinichi ; Nakamura, Seiji ; Kiyono, Hiroshi. / Allograft inflammatory factor 1 is a regulator of transcytosis in M cells. In: Nature communications. 2017 ; Vol. 8.
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