Allograft inflammatory factor 1 is a regulator of transcytosis in M cells

Sari Kishikawa, Shintaro S.S. Sato, Satoshi Kaneto, Shigeo Uchino, Shinichi Kohsaka, Seiji Nakamura, Hiroshi Kiyono

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

M cells in follicle-associated epithelium (FAE) are specialized antigen-sampling cells that take up intestinal luminal antigens. Transcription factor Spi-B regulates M-cell maturation, but the molecules that promote transcytosis within M cells are not fully identified. Here we show that mouse allograft inflammatory factor 1 (Aif1) is expressed by M cells and contributes to M-cell transcytosis. FAE in Aif1 -/- mice has suppressed uptake of particles and commensal bacteria, compared with wild-type mice. Translocation of Yersinia enterocolitica, but not of Salmonella enterica serovar Typhimurium, leading to the generation of antigen-specific IgA antibodies, is also diminished in Aif1-deficient mice. Although β1 integrin, which acts as a receptor for Y. enterocolitica via invasin protein, is expressed on the apical surface membranes of M cells, its active form is rarely found in Aif1 -/- mice. These findings show that Aif1 is important for bacterial and particle transcytosis in M cells.

Original languageEnglish
Article number14509
JournalNature communications
Volume8
DOIs
Publication statusPublished - Feb 22 2017

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

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