alpha6 integrin expression in esophageal carcinoma.

Y. Tanaka, Koshi Mimori, T. Shiraishi, Y. Ohkura, K. Takubo, K. Mafune, G. F. Barnard, Masaki Mori

Research output: Contribution to journalArticle

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Abstract

One of the genes that the authors have isolated by a differential display of hepatocellular carcinoma compared to adjacent liver is the alpha6 integrin. alpha6 integrin is the major adhesion receptor for laminin and is suggested to be involved in tumor cell invasion and metastasis. To our knowledge, however, there are no reports on alpha6 integrin expression in esophageal carcinoma. We thus conducted a study to determine its clinicopathologic significance in human esophageal carcinoma. The tumor/normal (T/N) ratio of alpha6 integrin expression was calculated by Northern hybridization in 45 cases of esophageal carcinoma. In selected cases the expression of the alpha6 integrin variants A and B was also investigated by reverse transcriptase-polymerase chain reaction, and immunostaining for alpha6 integrin was performed. The expression levels of alpha6 integrin mRNA in the tumor tissue were greater than those of the corresponding normal tissue in 39 of 45 cases (87%). The overexpression of alpha6 integrin was also recognized by immunostaining. Fifteen cases with a high T/N ratio demonstrated a deeper invasion into the esophageal wall than the 30 cases with a low T/N ratio. Although there was no significant difference, the 15 cases with a high T/N ratio had a tendency for a worse prognosis. The ratio of the two variants (alpha6A/alpha6B) did not show any relationship to survival. The findings imply that alpha6 integrin is overexpressed in human esophageal carcinomas and that alpha6 integrin may play an important role in esophageal tumor invasion.

Original languageEnglish
Pages (from-to)725-729
Number of pages5
JournalInternational journal of oncology
Volume16
Issue number4
Publication statusPublished - Jan 1 2000
Externally publishedYes

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Integrin alpha6
Carcinoma
Neoplasms
Laminin
Reverse Transcriptase Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Tanaka, Y., Mimori, K., Shiraishi, T., Ohkura, Y., Takubo, K., Mafune, K., ... Mori, M. (2000). alpha6 integrin expression in esophageal carcinoma. International journal of oncology, 16(4), 725-729.

alpha6 integrin expression in esophageal carcinoma. / Tanaka, Y.; Mimori, Koshi; Shiraishi, T.; Ohkura, Y.; Takubo, K.; Mafune, K.; Barnard, G. F.; Mori, Masaki.

In: International journal of oncology, Vol. 16, No. 4, 01.01.2000, p. 725-729.

Research output: Contribution to journalArticle

Tanaka, Y, Mimori, K, Shiraishi, T, Ohkura, Y, Takubo, K, Mafune, K, Barnard, GF & Mori, M 2000, 'alpha6 integrin expression in esophageal carcinoma.', International journal of oncology, vol. 16, no. 4, pp. 725-729.
Tanaka Y, Mimori K, Shiraishi T, Ohkura Y, Takubo K, Mafune K et al. alpha6 integrin expression in esophageal carcinoma. International journal of oncology. 2000 Jan 1;16(4):725-729.
Tanaka, Y. ; Mimori, Koshi ; Shiraishi, T. ; Ohkura, Y. ; Takubo, K. ; Mafune, K. ; Barnard, G. F. ; Mori, Masaki. / alpha6 integrin expression in esophageal carcinoma. In: International journal of oncology. 2000 ; Vol. 16, No. 4. pp. 725-729.
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