TY - JOUR
T1 - Alteration of cross-linking selectivity with the 2′-OMe analogue of 2-amino-6-vinylpurine and evaluation of antisense effects
AU - Imoto, Shuhei
AU - Hori, Tsuneaki
AU - Hagihara, Shinya
AU - Taniguchi, Yosuke
AU - Sasaki, Shigeki
AU - Nagatsugi, Fumi
N1 - Funding Information:
The authors are grateful for the support offered by Grant-in-Aid for Scientific Research (B) from Japan Society for the promotion of Science and CREST from Japan Science and Technology Agency .
PY - 2010/10
Y1 - 2010/10
N2 - We previously reported that oligodeoxynucleotides containing 2-amino-6-vinylpurine (2-AVP: 1) exhibit efficient selective cross-linking to cytosine. In this study, the 2′-OMe nucleoside analogue (2) of 2-AVP was designed in order to increase its affinity to RNA and enhance metabolic stability. It has been demonstrated that 2′-OMe oligonucleotides bearing 2 achieve highly selective cross-linking to the thymine base in DNA and show higher antisense effect on luciferase production in cell lysate.
AB - We previously reported that oligodeoxynucleotides containing 2-amino-6-vinylpurine (2-AVP: 1) exhibit efficient selective cross-linking to cytosine. In this study, the 2′-OMe nucleoside analogue (2) of 2-AVP was designed in order to increase its affinity to RNA and enhance metabolic stability. It has been demonstrated that 2′-OMe oligonucleotides bearing 2 achieve highly selective cross-linking to the thymine base in DNA and show higher antisense effect on luciferase production in cell lysate.
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U2 - 10.1016/j.bmcl.2010.08.027
DO - 10.1016/j.bmcl.2010.08.027
M3 - Article
C2 - 20817451
AN - SCOPUS:77956926527
VL - 20
SP - 6121
EP - 6124
JO - Bioorganic and Medicinal Chemistry Letters
JF - Bioorganic and Medicinal Chemistry Letters
SN - 0960-894X
IS - 20
ER -