Alterations in B-cell subsets in pediatric patients with early atopic dermatitis

Tali Czarnowicki, Hitokazu Esaki, Juana Gonzalez, Yael Renert-Yuval, Patrick Brunner, Margeaux Oliva, Yeriel Estrada, Hui Xu, Xiuzhong Zheng, Sreya Talasila, Isabel Haugh, Thy Huynh, Sarah Lyon, Gary Tran, Hugh Sampson, Mayte Suárez-Fariñas, James G. Krueger, Emma Guttman-Yassky, Amy S. Paller

Research output: Contribution to journalArticle

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Abstract

Background B cells undergo maturation and class-switching in response to antigen exposure and T-cell help. Early B-cell differentiation has not been defined in patients with early-onset atopic dermatitis (AD). Objective We sought to define the frequency of B-cell subsets associated with progressive B-cell maturation and IgE class-switching. Methods We studied 27 children and 34 adults with moderate-to-severe AD (mean SCORAD score, 55 and 65, respectively) and age-matched control subjects (15 children and 27 adults). IgD/CD27 and CD24/CD38 core gating systems and an 11-color flow cytometric panel were used to determine the frequencies of circulating B-cell subsets. Serum total and allergen-specific IgE (sIgE) levels were measured by using ImmunoCAP. Results Compared with adults, children showed T-cell predominance in the skin. Circulating CD19 + CD20 + B-cell counts were lower in patients with pediatric AD than in control subjects (24% vs 33%, P =.04), whereas CD3 + T-cell counts were higher (62% vs 52%, P =.05). A decreased B-cell/T-cell lymphocyte ratio with age was observed only in pediatric control subjects (r = −0.48, P =.07). In pediatric patients with AD, a positive correlation was observed between B-cell/T-cell ratio and nonswitched memory B-cell counts (r = 0.42, P =.03). Higher frequencies of positive sIgE levels were seen in pediatric patients with AD (P <.0001). Diverse sIgE levels correlated with SCORAD scores and age of pediatric patients with AD (P <.01). Positive correlations were observed between activated B-cell and memory T-cell counts (P <.02). In patients with AD, IgE sensitization to most allergens clustered with age, T H 1, T H 2, total IgE levels, and B-cell memory subsets. Conclusions Peripheral B and T cells are altered in pediatric patients with early AD, but T cells predominate in skin lesions.

Original languageEnglish
Pages (from-to)134-144.e9
JournalJournal of Allergy and Clinical Immunology
Volume140
Issue number1
DOIs
Publication statusPublished - Jul 1 2017

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B-Lymphocyte Subsets
Atopic Dermatitis
B-Lymphocytes
Pediatrics
Immunoglobulin E
T-Lymphocytes
Cell Count
Immunoglobulin Class Switching
Allergens
Immunoglobulin D
Skin
Cell Differentiation
Color
Lymphocytes
Antigens

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Czarnowicki, T., Esaki, H., Gonzalez, J., Renert-Yuval, Y., Brunner, P., Oliva, M., ... Paller, A. S. (2017). Alterations in B-cell subsets in pediatric patients with early atopic dermatitis. Journal of Allergy and Clinical Immunology, 140(1), 134-144.e9. https://doi.org/10.1016/j.jaci.2016.09.060

Alterations in B-cell subsets in pediatric patients with early atopic dermatitis. / Czarnowicki, Tali; Esaki, Hitokazu; Gonzalez, Juana; Renert-Yuval, Yael; Brunner, Patrick; Oliva, Margeaux; Estrada, Yeriel; Xu, Hui; Zheng, Xiuzhong; Talasila, Sreya; Haugh, Isabel; Huynh, Thy; Lyon, Sarah; Tran, Gary; Sampson, Hugh; Suárez-Fariñas, Mayte; Krueger, James G.; Guttman-Yassky, Emma; Paller, Amy S.

In: Journal of Allergy and Clinical Immunology, Vol. 140, No. 1, 01.07.2017, p. 134-144.e9.

Research output: Contribution to journalArticle

Czarnowicki, T, Esaki, H, Gonzalez, J, Renert-Yuval, Y, Brunner, P, Oliva, M, Estrada, Y, Xu, H, Zheng, X, Talasila, S, Haugh, I, Huynh, T, Lyon, S, Tran, G, Sampson, H, Suárez-Fariñas, M, Krueger, JG, Guttman-Yassky, E & Paller, AS 2017, 'Alterations in B-cell subsets in pediatric patients with early atopic dermatitis', Journal of Allergy and Clinical Immunology, vol. 140, no. 1, pp. 134-144.e9. https://doi.org/10.1016/j.jaci.2016.09.060
Czarnowicki T, Esaki H, Gonzalez J, Renert-Yuval Y, Brunner P, Oliva M et al. Alterations in B-cell subsets in pediatric patients with early atopic dermatitis. Journal of Allergy and Clinical Immunology. 2017 Jul 1;140(1):134-144.e9. https://doi.org/10.1016/j.jaci.2016.09.060
Czarnowicki, Tali ; Esaki, Hitokazu ; Gonzalez, Juana ; Renert-Yuval, Yael ; Brunner, Patrick ; Oliva, Margeaux ; Estrada, Yeriel ; Xu, Hui ; Zheng, Xiuzhong ; Talasila, Sreya ; Haugh, Isabel ; Huynh, Thy ; Lyon, Sarah ; Tran, Gary ; Sampson, Hugh ; Suárez-Fariñas, Mayte ; Krueger, James G. ; Guttman-Yassky, Emma ; Paller, Amy S. / Alterations in B-cell subsets in pediatric patients with early atopic dermatitis. In: Journal of Allergy and Clinical Immunology. 2017 ; Vol. 140, No. 1. pp. 134-144.e9.
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abstract = "Background B cells undergo maturation and class-switching in response to antigen exposure and T-cell help. Early B-cell differentiation has not been defined in patients with early-onset atopic dermatitis (AD). Objective We sought to define the frequency of B-cell subsets associated with progressive B-cell maturation and IgE class-switching. Methods We studied 27 children and 34 adults with moderate-to-severe AD (mean SCORAD score, 55 and 65, respectively) and age-matched control subjects (15 children and 27 adults). IgD/CD27 and CD24/CD38 core gating systems and an 11-color flow cytometric panel were used to determine the frequencies of circulating B-cell subsets. Serum total and allergen-specific IgE (sIgE) levels were measured by using ImmunoCAP. Results Compared with adults, children showed T-cell predominance in the skin. Circulating CD19 + CD20 + B-cell counts were lower in patients with pediatric AD than in control subjects (24{\%} vs 33{\%}, P =.04), whereas CD3 + T-cell counts were higher (62{\%} vs 52{\%}, P =.05). A decreased B-cell/T-cell lymphocyte ratio with age was observed only in pediatric control subjects (r = −0.48, P =.07). In pediatric patients with AD, a positive correlation was observed between B-cell/T-cell ratio and nonswitched memory B-cell counts (r = 0.42, P =.03). Higher frequencies of positive sIgE levels were seen in pediatric patients with AD (P <.0001). Diverse sIgE levels correlated with SCORAD scores and age of pediatric patients with AD (P <.01). Positive correlations were observed between activated B-cell and memory T-cell counts (P <.02). In patients with AD, IgE sensitization to most allergens clustered with age, T H 1, T H 2, total IgE levels, and B-cell memory subsets. Conclusions Peripheral B and T cells are altered in pediatric patients with early AD, but T cells predominate in skin lesions.",
author = "Tali Czarnowicki and Hitokazu Esaki and Juana Gonzalez and Yael Renert-Yuval and Patrick Brunner and Margeaux Oliva and Yeriel Estrada and Hui Xu and Xiuzhong Zheng and Sreya Talasila and Isabel Haugh and Thy Huynh and Sarah Lyon and Gary Tran and Hugh Sampson and Mayte Su{\'a}rez-Fari{\~n}as and Krueger, {James G.} and Emma Guttman-Yassky and Paller, {Amy S.}",
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AU - Czarnowicki, Tali

AU - Esaki, Hitokazu

AU - Gonzalez, Juana

AU - Renert-Yuval, Yael

AU - Brunner, Patrick

AU - Oliva, Margeaux

AU - Estrada, Yeriel

AU - Xu, Hui

AU - Zheng, Xiuzhong

AU - Talasila, Sreya

AU - Haugh, Isabel

AU - Huynh, Thy

AU - Lyon, Sarah

AU - Tran, Gary

AU - Sampson, Hugh

AU - Suárez-Fariñas, Mayte

AU - Krueger, James G.

AU - Guttman-Yassky, Emma

AU - Paller, Amy S.

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N2 - Background B cells undergo maturation and class-switching in response to antigen exposure and T-cell help. Early B-cell differentiation has not been defined in patients with early-onset atopic dermatitis (AD). Objective We sought to define the frequency of B-cell subsets associated with progressive B-cell maturation and IgE class-switching. Methods We studied 27 children and 34 adults with moderate-to-severe AD (mean SCORAD score, 55 and 65, respectively) and age-matched control subjects (15 children and 27 adults). IgD/CD27 and CD24/CD38 core gating systems and an 11-color flow cytometric panel were used to determine the frequencies of circulating B-cell subsets. Serum total and allergen-specific IgE (sIgE) levels were measured by using ImmunoCAP. Results Compared with adults, children showed T-cell predominance in the skin. Circulating CD19 + CD20 + B-cell counts were lower in patients with pediatric AD than in control subjects (24% vs 33%, P =.04), whereas CD3 + T-cell counts were higher (62% vs 52%, P =.05). A decreased B-cell/T-cell lymphocyte ratio with age was observed only in pediatric control subjects (r = −0.48, P =.07). In pediatric patients with AD, a positive correlation was observed between B-cell/T-cell ratio and nonswitched memory B-cell counts (r = 0.42, P =.03). Higher frequencies of positive sIgE levels were seen in pediatric patients with AD (P <.0001). Diverse sIgE levels correlated with SCORAD scores and age of pediatric patients with AD (P <.01). Positive correlations were observed between activated B-cell and memory T-cell counts (P <.02). In patients with AD, IgE sensitization to most allergens clustered with age, T H 1, T H 2, total IgE levels, and B-cell memory subsets. Conclusions Peripheral B and T cells are altered in pediatric patients with early AD, but T cells predominate in skin lesions.

AB - Background B cells undergo maturation and class-switching in response to antigen exposure and T-cell help. Early B-cell differentiation has not been defined in patients with early-onset atopic dermatitis (AD). Objective We sought to define the frequency of B-cell subsets associated with progressive B-cell maturation and IgE class-switching. Methods We studied 27 children and 34 adults with moderate-to-severe AD (mean SCORAD score, 55 and 65, respectively) and age-matched control subjects (15 children and 27 adults). IgD/CD27 and CD24/CD38 core gating systems and an 11-color flow cytometric panel were used to determine the frequencies of circulating B-cell subsets. Serum total and allergen-specific IgE (sIgE) levels were measured by using ImmunoCAP. Results Compared with adults, children showed T-cell predominance in the skin. Circulating CD19 + CD20 + B-cell counts were lower in patients with pediatric AD than in control subjects (24% vs 33%, P =.04), whereas CD3 + T-cell counts were higher (62% vs 52%, P =.05). A decreased B-cell/T-cell lymphocyte ratio with age was observed only in pediatric control subjects (r = −0.48, P =.07). In pediatric patients with AD, a positive correlation was observed between B-cell/T-cell ratio and nonswitched memory B-cell counts (r = 0.42, P =.03). Higher frequencies of positive sIgE levels were seen in pediatric patients with AD (P <.0001). Diverse sIgE levels correlated with SCORAD scores and age of pediatric patients with AD (P <.01). Positive correlations were observed between activated B-cell and memory T-cell counts (P <.02). In patients with AD, IgE sensitization to most allergens clustered with age, T H 1, T H 2, total IgE levels, and B-cell memory subsets. Conclusions Peripheral B and T cells are altered in pediatric patients with early AD, but T cells predominate in skin lesions.

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