Altered growth of human colon cancer cell lines disrupted at activated Ki-ras

Senji Shirasawa, Masanori Furuse, Nobuhiko Yokoyama, Takehiko Sasazuki

Research output: Contribution to journalArticle

552 Citations (Scopus)

Abstract

Point mutations that activate the Ki-ras proto-oncogene are present in about 50 percent of human colorectal tumors. To study the functional significance of these mutations, the activated Ki-ras genes in two human colon carcinoma cell lines, DLD-1 and HCT 116, were disrupted by homologous recombination. Compared with parental cells, cells disrupted at the activated Ki-ras gene were morphologically altered, lost the capacity for anchorage-independent growth, grew more slowly both in vitro and in nude mice, and showed reduced expression of c-myc. Thus, the activated Ki-ras gene plays a key role in colorectal tumorigenesis through altered cell differentiation and cell growth.

Original languageEnglish
Pages (from-to)85-88
Number of pages4
JournalScience
Volume260
Issue number5104
DOIs
Publication statusPublished - Jan 1 1993

Fingerprint

ras Genes
Colonic Neoplasms
Cell Line
Growth
Proto-Oncogenes
Homologous Recombination
Point Mutation
Nude Mice
Cell Differentiation
Colorectal Neoplasms
Colon
Carcinogenesis
Carcinoma
Mutation

All Science Journal Classification (ASJC) codes

  • General

Cite this

Shirasawa, S., Furuse, M., Yokoyama, N., & Sasazuki, T. (1993). Altered growth of human colon cancer cell lines disrupted at activated Ki-ras. Science, 260(5104), 85-88. https://doi.org/10.1126/science.8465203

Altered growth of human colon cancer cell lines disrupted at activated Ki-ras. / Shirasawa, Senji; Furuse, Masanori; Yokoyama, Nobuhiko; Sasazuki, Takehiko.

In: Science, Vol. 260, No. 5104, 01.01.1993, p. 85-88.

Research output: Contribution to journalArticle

Shirasawa, S, Furuse, M, Yokoyama, N & Sasazuki, T 1993, 'Altered growth of human colon cancer cell lines disrupted at activated Ki-ras', Science, vol. 260, no. 5104, pp. 85-88. https://doi.org/10.1126/science.8465203
Shirasawa, Senji ; Furuse, Masanori ; Yokoyama, Nobuhiko ; Sasazuki, Takehiko. / Altered growth of human colon cancer cell lines disrupted at activated Ki-ras. In: Science. 1993 ; Vol. 260, No. 5104. pp. 85-88.
@article{5525656d73684bc2b41fb25aeaba5fb9,
title = "Altered growth of human colon cancer cell lines disrupted at activated Ki-ras",
abstract = "Point mutations that activate the Ki-ras proto-oncogene are present in about 50 percent of human colorectal tumors. To study the functional significance of these mutations, the activated Ki-ras genes in two human colon carcinoma cell lines, DLD-1 and HCT 116, were disrupted by homologous recombination. Compared with parental cells, cells disrupted at the activated Ki-ras gene were morphologically altered, lost the capacity for anchorage-independent growth, grew more slowly both in vitro and in nude mice, and showed reduced expression of c-myc. Thus, the activated Ki-ras gene plays a key role in colorectal tumorigenesis through altered cell differentiation and cell growth.",
author = "Senji Shirasawa and Masanori Furuse and Nobuhiko Yokoyama and Takehiko Sasazuki",
year = "1993",
month = "1",
day = "1",
doi = "10.1126/science.8465203",
language = "English",
volume = "260",
pages = "85--88",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "5104",

}

TY - JOUR

T1 - Altered growth of human colon cancer cell lines disrupted at activated Ki-ras

AU - Shirasawa, Senji

AU - Furuse, Masanori

AU - Yokoyama, Nobuhiko

AU - Sasazuki, Takehiko

PY - 1993/1/1

Y1 - 1993/1/1

N2 - Point mutations that activate the Ki-ras proto-oncogene are present in about 50 percent of human colorectal tumors. To study the functional significance of these mutations, the activated Ki-ras genes in two human colon carcinoma cell lines, DLD-1 and HCT 116, were disrupted by homologous recombination. Compared with parental cells, cells disrupted at the activated Ki-ras gene were morphologically altered, lost the capacity for anchorage-independent growth, grew more slowly both in vitro and in nude mice, and showed reduced expression of c-myc. Thus, the activated Ki-ras gene plays a key role in colorectal tumorigenesis through altered cell differentiation and cell growth.

AB - Point mutations that activate the Ki-ras proto-oncogene are present in about 50 percent of human colorectal tumors. To study the functional significance of these mutations, the activated Ki-ras genes in two human colon carcinoma cell lines, DLD-1 and HCT 116, were disrupted by homologous recombination. Compared with parental cells, cells disrupted at the activated Ki-ras gene were morphologically altered, lost the capacity for anchorage-independent growth, grew more slowly both in vitro and in nude mice, and showed reduced expression of c-myc. Thus, the activated Ki-ras gene plays a key role in colorectal tumorigenesis through altered cell differentiation and cell growth.

UR - http://www.scopus.com/inward/record.url?scp=0027905014&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027905014&partnerID=8YFLogxK

U2 - 10.1126/science.8465203

DO - 10.1126/science.8465203

M3 - Article

C2 - 8465203

AN - SCOPUS:0027905014

VL - 260

SP - 85

EP - 88

JO - Science

JF - Science

SN - 0036-8075

IS - 5104

ER -