Altered growth of human colon cancer cell lines disrupted at activated Ki-ras

Senji Shirasawa, Masanori Furuse, Nobuhiko Yokoyama, Takehiko Sasazuki

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569 Citations (Scopus)

Abstract

Point mutations that activate the Ki-ras proto-oncogene are present in about 50 percent of human colorectal tumors. To study the functional significance of these mutations, the activated Ki-ras genes in two human colon carcinoma cell lines, DLD-1 and HCT 116, were disrupted by homologous recombination. Compared with parental cells, cells disrupted at the activated Ki-ras gene were morphologically altered, lost the capacity for anchorage-independent growth, grew more slowly both in vitro and in nude mice, and showed reduced expression of c-myc. Thus, the activated Ki-ras gene plays a key role in colorectal tumorigenesis through altered cell differentiation and cell growth.

Original languageEnglish
Pages (from-to)85-88
Number of pages4
JournalScience
Volume260
Issue number5104
DOIs
Publication statusPublished - 1993

All Science Journal Classification (ASJC) codes

  • General

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    Shirasawa, S., Furuse, M., Yokoyama, N., & Sasazuki, T. (1993). Altered growth of human colon cancer cell lines disrupted at activated Ki-ras. Science, 260(5104), 85-88. https://doi.org/10.1126/science.8465203