Abstract
Point mutations that activate the Ki-ras proto-oncogene are present in about 50 percent of human colorectal tumors. To study the functional significance of these mutations, the activated Ki-ras genes in two human colon carcinoma cell lines, DLD-1 and HCT 116, were disrupted by homologous recombination. Compared with parental cells, cells disrupted at the activated Ki-ras gene were morphologically altered, lost the capacity for anchorage-independent growth, grew more slowly both in vitro and in nude mice, and showed reduced expression of c-myc. Thus, the activated Ki-ras gene plays a key role in colorectal tumorigenesis through altered cell differentiation and cell growth.
| Original language | English |
|---|---|
| Pages (from-to) | 85-88 |
| Number of pages | 4 |
| Journal | Science |
| Volume | 260 |
| Issue number | 5104 |
| DOIs | |
| Publication status | Published - Jan 1 1993 |
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All Science Journal Classification (ASJC) codes
- General
Cite this
Altered growth of human colon cancer cell lines disrupted at activated Ki-ras. / Shirasawa, Senji; Furuse, Masanori; Yokoyama, Nobuhiko; Sasazuki, Takehiko.
In: Science, Vol. 260, No. 5104, 01.01.1993, p. 85-88.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Altered growth of human colon cancer cell lines disrupted at activated Ki-ras
AU - Shirasawa, Senji
AU - Furuse, Masanori
AU - Yokoyama, Nobuhiko
AU - Sasazuki, Takehiko
PY - 1993/1/1
Y1 - 1993/1/1
N2 - Point mutations that activate the Ki-ras proto-oncogene are present in about 50 percent of human colorectal tumors. To study the functional significance of these mutations, the activated Ki-ras genes in two human colon carcinoma cell lines, DLD-1 and HCT 116, were disrupted by homologous recombination. Compared with parental cells, cells disrupted at the activated Ki-ras gene were morphologically altered, lost the capacity for anchorage-independent growth, grew more slowly both in vitro and in nude mice, and showed reduced expression of c-myc. Thus, the activated Ki-ras gene plays a key role in colorectal tumorigenesis through altered cell differentiation and cell growth.
AB - Point mutations that activate the Ki-ras proto-oncogene are present in about 50 percent of human colorectal tumors. To study the functional significance of these mutations, the activated Ki-ras genes in two human colon carcinoma cell lines, DLD-1 and HCT 116, were disrupted by homologous recombination. Compared with parental cells, cells disrupted at the activated Ki-ras gene were morphologically altered, lost the capacity for anchorage-independent growth, grew more slowly both in vitro and in nude mice, and showed reduced expression of c-myc. Thus, the activated Ki-ras gene plays a key role in colorectal tumorigenesis through altered cell differentiation and cell growth.
UR - http://www.scopus.com/inward/record.url?scp=0027905014&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027905014&partnerID=8YFLogxK
U2 - 10.1126/science.8465203
DO - 10.1126/science.8465203
M3 - Article
C2 - 8465203
AN - SCOPUS:0027905014
VL - 260
SP - 85
EP - 88
JO - Science
JF - Science
SN - 0036-8075
IS - 5104
ER -