TY - JOUR
T1 - Altered growth of human colon cancer cell lines disrupted at activated Ki-ras
AU - Shirasawa, Senji
AU - Furuse, Masanori
AU - Yokoyama, Nobuhiko
AU - Sasazuki, Takehiko
PY - 1993
Y1 - 1993
N2 - Point mutations that activate the Ki-ras proto-oncogene are present in about 50 percent of human colorectal tumors. To study the functional significance of these mutations, the activated Ki-ras genes in two human colon carcinoma cell lines, DLD-1 and HCT 116, were disrupted by homologous recombination. Compared with parental cells, cells disrupted at the activated Ki-ras gene were morphologically altered, lost the capacity for anchorage-independent growth, grew more slowly both in vitro and in nude mice, and showed reduced expression of c-myc. Thus, the activated Ki-ras gene plays a key role in colorectal tumorigenesis through altered cell differentiation and cell growth.
AB - Point mutations that activate the Ki-ras proto-oncogene are present in about 50 percent of human colorectal tumors. To study the functional significance of these mutations, the activated Ki-ras genes in two human colon carcinoma cell lines, DLD-1 and HCT 116, were disrupted by homologous recombination. Compared with parental cells, cells disrupted at the activated Ki-ras gene were morphologically altered, lost the capacity for anchorage-independent growth, grew more slowly both in vitro and in nude mice, and showed reduced expression of c-myc. Thus, the activated Ki-ras gene plays a key role in colorectal tumorigenesis through altered cell differentiation and cell growth.
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U2 - 10.1126/science.8465203
DO - 10.1126/science.8465203
M3 - Article
C2 - 8465203
AN - SCOPUS:0027905014
VL - 260
SP - 85
EP - 88
JO - Science
JF - Science
SN - 0036-8075
IS - 5104
ER -