Alternation of extracellular matrix remodeling and apoptosis by activation of the aryl hydrocarbon receptor pathway in human periodontal ligament cells

Atsushi Tomokiyo, Hidefumi Maeda, Shinsuke Fujii, Satoshi Monnouchi, Naohisa Wada, Kiyomi Hori, Katsuaki Koori, Naohide Yamamoto, Yoko Teramatsu, Akifumi Akamine

Research output: Contribution to journalArticle

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Abstract

It is well known that the aryl hydrocarbon receptor (AhR) is involved in the toxicity of halogenated aromatic hydrocarbons (HAH) and polycyclic aromatic hydrocarbons (PAH). Recent experiments have shown the induction of impaired tooth and hard-tissue formation by AhR pathway activation, however, the effect on periodontal ligament (PDL) tissue remains unclear. Here, we investigated the effects of benzo(a)pyrene (BaP), a member of PAH, on the extracellular matrix (ECM) remodeling-related molecules, collagen type I (COL-I), matrix metalloproteinase-1 (MMP-1), alpha-smooth muscle actin (α-SMA) expression, and apoptosis in two different human periodontal ligament cells (HPDLCs). The transduction of AhR from the cytoplasm to the nucleus and the increase of AhR-responsive genes; that is, cytochrome P450 1A1 (CYP1A1), cytochrome P450 1B1 (CYP1B1), and aryl-hydrocarbon receptor repressor (AhRR), expression was induced by BaP exposure in both HPDLCs. BaP treatment significantly enhanced MMP-1 mRNA expression and MMP-1 protein production, while markedly suppressing COL-I and a-SMA mRNA expression in both HPDLCs. Furthermore, these BaP-treated HPDLCs fell into apoptotic cell death as evidenced by induction in annexin V and caspase-3/7 staining and reduction of total cell number and Bcl-2 mRNA expression. Thus, BaP exposure altered the expression of ECM-related molecules and induced apoptosis in HPDLCs through activation of the AhR pathway. Overactivity of the AhR pathway may induce an inappropriate turnover of PDL tissue via disordered ECM remodeling and apoptosis in PDL cells.

Original languageEnglish
Pages (from-to)3093-3103
Number of pages11
JournalJournal of Cellular Biochemistry
Volume113
Issue number10
DOIs
Publication statusPublished - Jan 1 2012

Fingerprint

Aryl Hydrocarbon Receptors
Periodontal Ligament
Ligaments
Extracellular Matrix
Chemical activation
Apoptosis
Matrix Metalloproteinase 1
Polycyclic Aromatic Hydrocarbons
Tissue
Collagen Type I
Cytochrome P-450 Enzyme System
Messenger RNA
Halogenated Hydrocarbons
Caspase 7
Aromatic Hydrocarbons
Molecules
Annexin A5
Aromatic hydrocarbons
Benzo(a)pyrene
Cell death

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Alternation of extracellular matrix remodeling and apoptosis by activation of the aryl hydrocarbon receptor pathway in human periodontal ligament cells. / Tomokiyo, Atsushi; Maeda, Hidefumi; Fujii, Shinsuke; Monnouchi, Satoshi; Wada, Naohisa; Hori, Kiyomi; Koori, Katsuaki; Yamamoto, Naohide; Teramatsu, Yoko; Akamine, Akifumi.

In: Journal of Cellular Biochemistry, Vol. 113, No. 10, 01.01.2012, p. 3093-3103.

Research output: Contribution to journalArticle

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