Alternative mRNA Splicing in Three Venom Families Underlying a Possible Production of Divergent Venom Proteins of the Habu Snake, Protobothrops flavoviridis

Tomohisa Ogawa, Naoko Oda-Ueda, Kanako Hisata, Hitomi Nakamura, Takahito Chijiwa, Shousaku Hattori, Akiko Isomoto, Haruki Yugeta, Shinichi Yamasaki, Yasuyuki Fukumaki, Motonori Ohno, Noriyuki Satoh, Hiroki Shibata

Research output: Contribution to journalArticle

Abstract

Snake venoms are complex mixtures of toxic proteins encoded by various gene families that function synergistically to incapacitate prey. A huge repertoire of snake venom genes and proteins have been reported, and alternative splicing is suggested to be involved in the production of divergent gene transcripts. However, a genome-wide survey of the transcript repertoire and the extent of alternative splicing still remains to be determined. In this study, the comprehensive analysis of transcriptomes in the venom gland was achieved by using PacBio sequencing. Extensive alternative splicing was observed in three venom protein gene families, metalloproteinase (MP), serine protease (SP), and vascular endothelial growth factors (VEGF). Eleven MP and SP genes and a VEGF gene are expressed as a total of 81, 61, and 8 transcript variants, respectively. In the MP gene family, individual genes are transcribed into different classes of MPs by alternative splicing. We also observed trans-splicing among the clustered SP genes. No other venom genes as well as non-venom counterpart genes exhibited alternative splicing. Our results thus indicate a potential contribution of mRNA alternative and trans-splicing in the production of highly variable transcripts of venom genes in the habu snake.

Original languageEnglish
JournalToxins
Volume11
Issue number10
DOIs
Publication statusPublished - Oct 9 2019

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Trimeresurus
Snakes
Venoms
Alternative Splicing
Genes
Messenger RNA
Proteins
Metalloproteases
Serine Proteases
Vascular Endothelial Growth Factors
Trans-Splicing
Snake Venoms
Poisons
Gene Expression Profiling
Complex Mixtures

All Science Journal Classification (ASJC) codes

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Alternative mRNA Splicing in Three Venom Families Underlying a Possible Production of Divergent Venom Proteins of the Habu Snake, Protobothrops flavoviridis. / Ogawa, Tomohisa; Oda-Ueda, Naoko; Hisata, Kanako; Nakamura, Hitomi; Chijiwa, Takahito; Hattori, Shousaku; Isomoto, Akiko; Yugeta, Haruki; Yamasaki, Shinichi; Fukumaki, Yasuyuki; Ohno, Motonori; Satoh, Noriyuki; Shibata, Hiroki.

In: Toxins, Vol. 11, No. 10, 09.10.2019.

Research output: Contribution to journalArticle

Ogawa, T, Oda-Ueda, N, Hisata, K, Nakamura, H, Chijiwa, T, Hattori, S, Isomoto, A, Yugeta, H, Yamasaki, S, Fukumaki, Y, Ohno, M, Satoh, N & Shibata, H 2019, 'Alternative mRNA Splicing in Three Venom Families Underlying a Possible Production of Divergent Venom Proteins of the Habu Snake, Protobothrops flavoviridis', Toxins, vol. 11, no. 10. https://doi.org/10.3390/toxins11100581
Ogawa, Tomohisa ; Oda-Ueda, Naoko ; Hisata, Kanako ; Nakamura, Hitomi ; Chijiwa, Takahito ; Hattori, Shousaku ; Isomoto, Akiko ; Yugeta, Haruki ; Yamasaki, Shinichi ; Fukumaki, Yasuyuki ; Ohno, Motonori ; Satoh, Noriyuki ; Shibata, Hiroki. / Alternative mRNA Splicing in Three Venom Families Underlying a Possible Production of Divergent Venom Proteins of the Habu Snake, Protobothrops flavoviridis. In: Toxins. 2019 ; Vol. 11, No. 10.
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