Ambuic acid inhibits the biosynthesis of cyclic peptide quormones in gram-positive bacteria

Jiro Nakayama, Yumi Uemura, Kenzo Nishiguchi, Norito Yoshimura, Yasuhiro Igarashi, Kenji Sonomoto

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Quorum sensing is a cell-density-dependent regulatory system in gram-positive bacteria and is often regulated by cyclic peptides called "quormones," which function as extracellular communication signals. With an aim to discover an antipathogenic agent targeting quorum sensing in gram-positive bacteria, we screened 153 samples of fungal butanol extracts with the guidance of the inhibition of quorum-sensing-mediated gelatinase production in Enterococcus faecalis. Following the screenings, we found that ambuic acid, a known secondary fungal metabolite, inhibited the quorum-sensing-mediated gelatinase production without influencing the growth of E. faecalis. We further demonstrated that ambuic acid targeted the biosynthesis of a cyclic peptide quormone called gelatinase biosynthesis-activating pheromone. Furthermore, ambuic acid also inhibited the biosynthesis of the cyclic peptide quormones of Staphylococcus aureus and Listeria innocua. These results suggest the potential use of ambuic acid as a lead compound of antipathogenic drugs that target the quorum-sensing-mediated virulence expression of gram-positive bacteria.

Original languageEnglish
Pages (from-to)580-586
Number of pages7
JournalAntimicrobial Agents and Chemotherapy
Volume53
Issue number2
DOIs
Publication statusPublished - Feb 1 2009

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Quorum Sensing
Cyclic Peptides
Gram-Positive Bacteria
Gelatinases
Enterococcus faecalis
Butanols
Listeria
Virulence
Staphylococcus aureus
Cell Count
ambuic acid
Growth
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

Ambuic acid inhibits the biosynthesis of cyclic peptide quormones in gram-positive bacteria. / Nakayama, Jiro; Uemura, Yumi; Nishiguchi, Kenzo; Yoshimura, Norito; Igarashi, Yasuhiro; Sonomoto, Kenji.

In: Antimicrobial Agents and Chemotherapy, Vol. 53, No. 2, 01.02.2009, p. 580-586.

Research output: Contribution to journalArticle

Nakayama, Jiro ; Uemura, Yumi ; Nishiguchi, Kenzo ; Yoshimura, Norito ; Igarashi, Yasuhiro ; Sonomoto, Kenji. / Ambuic acid inhibits the biosynthesis of cyclic peptide quormones in gram-positive bacteria. In: Antimicrobial Agents and Chemotherapy. 2009 ; Vol. 53, No. 2. pp. 580-586.
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