Amelioration of experimental allergic rhinitis with suppression of topical immune responses by lack of IL-27/WSX-1 signaling

Yohei Shimanoe, Yoshiyuki Miyazaki, Hiromitsu Hara, Akira Inokuchi, Hiroki Yoshida

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

BackgroundAllergic rhinitis is 1 of the most common atopic diseases with strong similarity to asthma. Interleukin (IL) 27 is an immunosuppressive cytokine, and lack of the IL-27 receptor (WSX-1) resulted in exacerbation of allergic airway hyperresponsiveness. Objective: To address the role of IL-27/WSX-1 in the rhinitis model compared with the asthma model. Methods: Wild-type or WSX-1 / female mice were immunized intraperitoneally 4 times with ovalbumin adsorbed to aluminum potassium sulfate at a 1-week interval. The sensitized mice were then challenged for 14 days with ovalbumin intranasally from days 22 to 35. Clinical scores, serum antigen specific IgE levels, and cytokine production in the nasal lavage fluid were examined. Cytokine and chemokine expression in the cervical lymph nodes, nasopharynx-associated lymphoid tissues, and nasal mucosa was also examined. Results: WSX-1/ mice developed augmented immune responses in the serum (IgE production), cervical lymph nodes (cytokine and chemokine expression), and nasopharynx-associated lymphoid tissues (cytokine and chemokine expression), whereas local responses, such as clinical scores and nasal lavage fluid cytokine production, were reduced in WSX-1/ mice. Expression of some chemokines was also reduced in the nasal mucosal tissues of WSX-1 / mice. Conclusion: In contrast to the immunosuppressive role observed in the asthma model, IL-27 WSX-1 topically plays an exacerbating role in the pathogenesis of allergic rhinitis, presumably through differential expression of chemokines.

Original languageEnglish
Pages (from-to)223-232
Number of pages10
JournalAnnals of Allergy, Asthma and Immunology
Volume102
Issue number3
DOIs
Publication statusPublished - Jan 1 2009
Externally publishedYes

Fingerprint

Interleukin-27
Chemokines
Cytokines
Nasal Lavage Fluid
Nasopharynx
Asthma
Ovalbumin
Lymphoid Tissue
Immunosuppressive Agents
Rhinitis
Immunoglobulin E
Lymph Nodes
Interleukin Receptors
Nasal Mucosa
Serum
Nose
Allergic Rhinitis
Mucous Membrane
Antigens

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Pulmonary and Respiratory Medicine

Cite this

Amelioration of experimental allergic rhinitis with suppression of topical immune responses by lack of IL-27/WSX-1 signaling. / Shimanoe, Yohei; Miyazaki, Yoshiyuki; Hara, Hiromitsu; Inokuchi, Akira; Yoshida, Hiroki.

In: Annals of Allergy, Asthma and Immunology, Vol. 102, No. 3, 01.01.2009, p. 223-232.

Research output: Contribution to journalArticle

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