Ameloblastin, an Extracellular Matrix Protein, Affects Long Bone Growth and Mineralization

Xuanyu Lu, Satoshi Fukumoto, Yoshihiko Yamada, Carla A. Evans, Thomas G.H. Diekwisch, Xianghong Luan

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Matrix molecules such as the enamel-related calcium-binding phosphoprotein ameloblastin (AMBN) are expressed in multiple tissues, including teeth, bones, and cartilage. Here we have asked whether AMBN is of functional importance for timely long bone development and, if so, how it exerts its function related to osteogenesis. Adolescent AMBN-deficient mice (AMBNΔ5-6) suffered from a 33% to 38% reduction in femur length and an 8.4% shorter trunk spinal column when compared with WT controls, whereas there was no difference between adult animals. On a cellular level, AMBN truncation resulted in a shortened growth plate and a 41% to 49% reduction in the number of proliferating tibia chondrocytes and osteoblasts. Bone marrow stromal cells (BMSCs) isolated from AMBN mutant mice displayed defects in proliferation and differentiation potential as well as cytoskeleton organization. Osteogenesis-related growth factors, such as insulin-like growth factor 1 (IGF1) and BMP7, were also significantly (46% to 73%) reduced in AMBN-deficient BMSCs. Addition of exogenous AMBN restored cytoskeleton structures in AMBN mutant BMSCs and resulted in a dramatic 400% to 600% increase in BMP2, BMP7, and Col1A expression. Block of RhoA diminished the effect of AMBN on osteogenic growth factor and matrix protein gene expression. Addition of exogenous BMP7 and IGF1 rescued the proliferation and differentiation potential of AMBN-deficient BMSCs. Confirming the effects of AMBN on long bone growth, back-crossing of mutant mice with full-length AMBN overexpressors resulted in a complete rescue of AMBNΔ5-6 bone defects. Together, these data indicate that AMBN affects extracellular matrix production and cell adhesion properties in the long bone growth plate, resulting in altered cytoskeletal dynamics, increased osteogenesis-related gene expression, as well as osteoblast and chondrocyte proliferation. We propose that AMBN facilitates rapid long bone growth and an important growth spurt during the skeletogenesis of adolescent tooth-bearing vertebrates.

Original languageEnglish
Pages (from-to)1235-1246
Number of pages12
JournalJournal of Bone and Mineral Research
Volume31
Issue number6
DOIs
Publication statusPublished - Jun 1 2016
Externally publishedYes

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Physiologic Calcification
Extracellular Matrix Proteins
Bone Development
Mesenchymal Stromal Cells
Osteogenesis
Growth Plate
Somatomedins
Chondrocytes
Cytoskeleton
Osteoblasts
Intercellular Signaling Peptides and Proteins
Tooth
Bone Plates
Gene Expression
Bone and Bones
Phosphoproteins
Dental Enamel
Tibia
Cell Adhesion
Femur

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Orthopedics and Sports Medicine

Cite this

Ameloblastin, an Extracellular Matrix Protein, Affects Long Bone Growth and Mineralization. / Lu, Xuanyu; Fukumoto, Satoshi; Yamada, Yoshihiko; Evans, Carla A.; Diekwisch, Thomas G.H.; Luan, Xianghong.

In: Journal of Bone and Mineral Research, Vol. 31, No. 6, 01.06.2016, p. 1235-1246.

Research output: Contribution to journalArticle

Lu, Xuanyu ; Fukumoto, Satoshi ; Yamada, Yoshihiko ; Evans, Carla A. ; Diekwisch, Thomas G.H. ; Luan, Xianghong. / Ameloblastin, an Extracellular Matrix Protein, Affects Long Bone Growth and Mineralization. In: Journal of Bone and Mineral Research. 2016 ; Vol. 31, No. 6. pp. 1235-1246.
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