Amelogenin-mediated regulation of osteoclastogenesis, and periodontal cell proliferation and migration

J. Hatakeyama, D. Philp, Y. Hatakeyama, Naoto Haruyama, L. Shum, M. A. Aragon, Z. Yuan, C. W. Gibson, T. Sreenath, H. K. Kleinman, A. B. Kulkami

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

We previously reported that amelogenin isoforms M180 and leucine-rich amelogenin peptide (LRAP) are expressed in the periodontal region, and that their absence is associated with increased cementum defects in amelogenin-knockout (KO) mice. The aim of the present study was to characterize the functions of these isoforms in osteoclastogenesis and in the proliferation and migration of cementoblast/periodontal ligament cells. The co-cultures of wild-type (WT) osteoclast progenitor and KO cementoblast/periodontal ligament cells displayed more tartrate-resistant acid phosphatase (TRAP)-positive cells than the co-cultures of WT cells. The addition of LRAP to both co-cultures significantly reduced RANKL expression and the TRAP-positive cells. Proliferation and migration rates of the KO cementoblast/periodontal ligament cells were lower than those of WT cells and increased with the addition of either LRAP or P172 (a porcine homolog of mouse M180). Thus, we demonstrate the regulation of osteoclastogenesis by LRAP, and the proliferation and migration of cementoblast/periodontal ligament cells by LRAP and P172.

Original languageEnglish
Pages (from-to)144-149
Number of pages6
JournalJournal of Dental Research
Volume85
Issue number2
DOIs
Publication statusPublished - Feb 1 2006
Externally publishedYes

Fingerprint

Amelogenin
Osteogenesis
Dental Cementum
Cell Movement
Cell Proliferation
Periodontal Ligament
Coculture Techniques
Protein Isoforms
Osteoclasts
Knockout Mice
Swine
Cell Culture Techniques
leucine-rich amelogenin peptide

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Amelogenin-mediated regulation of osteoclastogenesis, and periodontal cell proliferation and migration. / Hatakeyama, J.; Philp, D.; Hatakeyama, Y.; Haruyama, Naoto; Shum, L.; Aragon, M. A.; Yuan, Z.; Gibson, C. W.; Sreenath, T.; Kleinman, H. K.; Kulkami, A. B.

In: Journal of Dental Research, Vol. 85, No. 2, 01.02.2006, p. 144-149.

Research output: Contribution to journalArticle

Hatakeyama, J, Philp, D, Hatakeyama, Y, Haruyama, N, Shum, L, Aragon, MA, Yuan, Z, Gibson, CW, Sreenath, T, Kleinman, HK & Kulkami, AB 2006, 'Amelogenin-mediated regulation of osteoclastogenesis, and periodontal cell proliferation and migration', Journal of Dental Research, vol. 85, no. 2, pp. 144-149. https://doi.org/10.1177/154405910608500206
Hatakeyama, J. ; Philp, D. ; Hatakeyama, Y. ; Haruyama, Naoto ; Shum, L. ; Aragon, M. A. ; Yuan, Z. ; Gibson, C. W. ; Sreenath, T. ; Kleinman, H. K. ; Kulkami, A. B. / Amelogenin-mediated regulation of osteoclastogenesis, and periodontal cell proliferation and migration. In: Journal of Dental Research. 2006 ; Vol. 85, No. 2. pp. 144-149.
@article{fbc4a1d1806449b1aa1f2b7069ed056b,
title = "Amelogenin-mediated regulation of osteoclastogenesis, and periodontal cell proliferation and migration",
abstract = "We previously reported that amelogenin isoforms M180 and leucine-rich amelogenin peptide (LRAP) are expressed in the periodontal region, and that their absence is associated with increased cementum defects in amelogenin-knockout (KO) mice. The aim of the present study was to characterize the functions of these isoforms in osteoclastogenesis and in the proliferation and migration of cementoblast/periodontal ligament cells. The co-cultures of wild-type (WT) osteoclast progenitor and KO cementoblast/periodontal ligament cells displayed more tartrate-resistant acid phosphatase (TRAP)-positive cells than the co-cultures of WT cells. The addition of LRAP to both co-cultures significantly reduced RANKL expression and the TRAP-positive cells. Proliferation and migration rates of the KO cementoblast/periodontal ligament cells were lower than those of WT cells and increased with the addition of either LRAP or P172 (a porcine homolog of mouse M180). Thus, we demonstrate the regulation of osteoclastogenesis by LRAP, and the proliferation and migration of cementoblast/periodontal ligament cells by LRAP and P172.",
author = "J. Hatakeyama and D. Philp and Y. Hatakeyama and Naoto Haruyama and L. Shum and Aragon, {M. A.} and Z. Yuan and Gibson, {C. W.} and T. Sreenath and Kleinman, {H. K.} and Kulkami, {A. B.}",
year = "2006",
month = "2",
day = "1",
doi = "10.1177/154405910608500206",
language = "English",
volume = "85",
pages = "144--149",
journal = "Journal of Dental Research",
issn = "0022-0345",
publisher = "SAGE Publications Inc.",
number = "2",

}

TY - JOUR

T1 - Amelogenin-mediated regulation of osteoclastogenesis, and periodontal cell proliferation and migration

AU - Hatakeyama, J.

AU - Philp, D.

AU - Hatakeyama, Y.

AU - Haruyama, Naoto

AU - Shum, L.

AU - Aragon, M. A.

AU - Yuan, Z.

AU - Gibson, C. W.

AU - Sreenath, T.

AU - Kleinman, H. K.

AU - Kulkami, A. B.

PY - 2006/2/1

Y1 - 2006/2/1

N2 - We previously reported that amelogenin isoforms M180 and leucine-rich amelogenin peptide (LRAP) are expressed in the periodontal region, and that their absence is associated with increased cementum defects in amelogenin-knockout (KO) mice. The aim of the present study was to characterize the functions of these isoforms in osteoclastogenesis and in the proliferation and migration of cementoblast/periodontal ligament cells. The co-cultures of wild-type (WT) osteoclast progenitor and KO cementoblast/periodontal ligament cells displayed more tartrate-resistant acid phosphatase (TRAP)-positive cells than the co-cultures of WT cells. The addition of LRAP to both co-cultures significantly reduced RANKL expression and the TRAP-positive cells. Proliferation and migration rates of the KO cementoblast/periodontal ligament cells were lower than those of WT cells and increased with the addition of either LRAP or P172 (a porcine homolog of mouse M180). Thus, we demonstrate the regulation of osteoclastogenesis by LRAP, and the proliferation and migration of cementoblast/periodontal ligament cells by LRAP and P172.

AB - We previously reported that amelogenin isoforms M180 and leucine-rich amelogenin peptide (LRAP) are expressed in the periodontal region, and that their absence is associated with increased cementum defects in amelogenin-knockout (KO) mice. The aim of the present study was to characterize the functions of these isoforms in osteoclastogenesis and in the proliferation and migration of cementoblast/periodontal ligament cells. The co-cultures of wild-type (WT) osteoclast progenitor and KO cementoblast/periodontal ligament cells displayed more tartrate-resistant acid phosphatase (TRAP)-positive cells than the co-cultures of WT cells. The addition of LRAP to both co-cultures significantly reduced RANKL expression and the TRAP-positive cells. Proliferation and migration rates of the KO cementoblast/periodontal ligament cells were lower than those of WT cells and increased with the addition of either LRAP or P172 (a porcine homolog of mouse M180). Thus, we demonstrate the regulation of osteoclastogenesis by LRAP, and the proliferation and migration of cementoblast/periodontal ligament cells by LRAP and P172.

UR - http://www.scopus.com/inward/record.url?scp=33744780170&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33744780170&partnerID=8YFLogxK

U2 - 10.1177/154405910608500206

DO - 10.1177/154405910608500206

M3 - Article

C2 - 16434732

AN - SCOPUS:33744780170

VL - 85

SP - 144

EP - 149

JO - Journal of Dental Research

JF - Journal of Dental Research

SN - 0022-0345

IS - 2

ER -