Amiloride inhibition on NaCl responses of the chorda tympani nerve in two 129 substrains of mice, 129P3/J and 129X1/SvJ

Tadahiro Ohkuri, Keiko Nakano, Noriatsu Shigemura, Ryusuke Yoshida, Yuzo Ninomiya

Research output: Contribution to journalArticle

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Abstract

Amiloride, a sodium channel blocker, is known to suppress NaCl responses of the chorda tympani (CT) nerve in various mammalian species. In mice, the NaCl suppressing effect of amiloride is reported to differ among strains. In C57BL mice, amiloride inhibits NaCl responses to about 50% of control, whereas no such clear suppression was evident in prior studies with 129 mice. However, evidence from behavioral studies is not entirely consistent with this. Recently, it has been found that genetic backgrounds of 129 mice differ within substrains. 129X1/SvJ (formerly 129/SvJ) mice differ from the 129P3/J (formerly 129/J) strain by 25% of sequence length polymorphisms. Therefore, we examined possible substrain difference between 129P3/J and 129X1/SvJ mice in the amiloride sensitivity of electrophysiologically recorded NaCl responses. Amiloride significantly suppressed CT responses to NaCl without affecting responses to KCl both in 129P3/J and 129X1/SvJ mice. However, the magnitude of the amiloride inhibition was significantly larger (∼50% of control in response to 0.01-1.0 M NaCl by 100 μM amiloride) in 129X1/SvJ than in 129P3/J mice (∼20% of control in response to 0.03-0.3 M NaCl by 100 μM amiloride). Threshold amiloride concentration for suppression of responses to 0.3 M NaCl was 30 μM in 129P3/J mice, which was higher than that in 129X1/SvJ mice (10 μM). In 129X1/SvJ mice, the threshold amiloride concentration eliciting inhibition of NaCl responses and the magnitude of the inhibition were comparable with those in C57BL/6 mice. These results suggest that amiloride sensitivity of NaCl responses differs even among the 129 substrains, 129P3/J and 129 X1/SvJ, and the substrain difference of 129 mice in amiloride sensitivity is as large as that between two inbred strains (129P3/J and C57BL/6).

Original languageEnglish
Pages (from-to)565-572
Number of pages8
JournalChemical Senses
Volume31
Issue number6
DOIs
Publication statusPublished - Jul 1 2006

Fingerprint

Chorda Tympani Nerve
129 Strain Mouse
Tympanis
Amiloride
nerve tissue
mice
Inbred C57BL Mouse
Chorda
Sodium Channel Blockers
sodium channels

All Science Journal Classification (ASJC) codes

  • Physiology
  • Sensory Systems
  • Physiology (medical)
  • Behavioral Neuroscience

Cite this

Amiloride inhibition on NaCl responses of the chorda tympani nerve in two 129 substrains of mice, 129P3/J and 129X1/SvJ. / Ohkuri, Tadahiro; Nakano, Keiko; Shigemura, Noriatsu; Yoshida, Ryusuke; Ninomiya, Yuzo.

In: Chemical Senses, Vol. 31, No. 6, 01.07.2006, p. 565-572.

Research output: Contribution to journalArticle

Ohkuri, Tadahiro ; Nakano, Keiko ; Shigemura, Noriatsu ; Yoshida, Ryusuke ; Ninomiya, Yuzo. / Amiloride inhibition on NaCl responses of the chorda tympani nerve in two 129 substrains of mice, 129P3/J and 129X1/SvJ. In: Chemical Senses. 2006 ; Vol. 31, No. 6. pp. 565-572.
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abstract = "Amiloride, a sodium channel blocker, is known to suppress NaCl responses of the chorda tympani (CT) nerve in various mammalian species. In mice, the NaCl suppressing effect of amiloride is reported to differ among strains. In C57BL mice, amiloride inhibits NaCl responses to about 50{\%} of control, whereas no such clear suppression was evident in prior studies with 129 mice. However, evidence from behavioral studies is not entirely consistent with this. Recently, it has been found that genetic backgrounds of 129 mice differ within substrains. 129X1/SvJ (formerly 129/SvJ) mice differ from the 129P3/J (formerly 129/J) strain by 25{\%} of sequence length polymorphisms. Therefore, we examined possible substrain difference between 129P3/J and 129X1/SvJ mice in the amiloride sensitivity of electrophysiologically recorded NaCl responses. Amiloride significantly suppressed CT responses to NaCl without affecting responses to KCl both in 129P3/J and 129X1/SvJ mice. However, the magnitude of the amiloride inhibition was significantly larger (∼50{\%} of control in response to 0.01-1.0 M NaCl by 100 μM amiloride) in 129X1/SvJ than in 129P3/J mice (∼20{\%} of control in response to 0.03-0.3 M NaCl by 100 μM amiloride). Threshold amiloride concentration for suppression of responses to 0.3 M NaCl was 30 μM in 129P3/J mice, which was higher than that in 129X1/SvJ mice (10 μM). In 129X1/SvJ mice, the threshold amiloride concentration eliciting inhibition of NaCl responses and the magnitude of the inhibition were comparable with those in C57BL/6 mice. These results suggest that amiloride sensitivity of NaCl responses differs even among the 129 substrains, 129P3/J and 129 X1/SvJ, and the substrain difference of 129 mice in amiloride sensitivity is as large as that between two inbred strains (129P3/J and C57BL/6).",
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