Amino Acid Sequence of Chymotrypsin Inhibitor ECI from the Seeds of Erythrina Variegata (LINN.) var. Orientalis

Makoto Kimura, Yoshiaki Kouzuma, Nobuyuki Yamasaki

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The amino acids of the chymotrypsin inhibitor (ECI) from the Erythrina variegata seeds have been sequenced. The sequence was solved by analysis of peptides derived from the protein by enzymatic digestions with trypsin and Staphylococcus aureus V8 proteinase, as well as by chemical cleavage with o-iodosobenzoic acid. The ECI consists of 179 amino acid residues with a pyroglutamic acid as the N-terminal residue and has a calculated molecular weight of 19, 791. Comparison of this sequence with the sequences of the two trypsin inhibitors, ETIa and ETIb, from the E. variegata seeds shows that about 60% of the residues of ECI are identical to those of ETIa and ETIb and that the reactive sites, Arg63, in ETIa and ETIb change to Leu64 in ECI.

Original languageEnglish
Pages (from-to)102-106
Number of pages5
JournalBioscience, Biotechnology and Biochemistry
Volume57
Issue number1
DOIs
Publication statusPublished - Jan 1 1993

Fingerprint

Erythrina
Chymotrypsin
Seed
Amino Acid Sequence
Seeds
Pyrrolidonecarboxylic Acid
Amino Acids
Trypsin Inhibitors
Trypsin
Proteolysis
Staphylococcus aureus
Catalytic Domain
Peptide Hydrolases
Molecular Weight
Molecular weight
Peptides
Acids
Proteins

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Amino Acid Sequence of Chymotrypsin Inhibitor ECI from the Seeds of Erythrina Variegata (LINN.) var. Orientalis. / Kimura, Makoto; Kouzuma, Yoshiaki; Yamasaki, Nobuyuki.

In: Bioscience, Biotechnology and Biochemistry, Vol. 57, No. 1, 01.01.1993, p. 102-106.

Research output: Contribution to journalArticle

Kimura, Makoto ; Kouzuma, Yoshiaki ; Yamasaki, Nobuyuki. / Amino Acid Sequence of Chymotrypsin Inhibitor ECI from the Seeds of Erythrina Variegata (LINN.) var. Orientalis. In: Bioscience, Biotechnology and Biochemistry. 1993 ; Vol. 57, No. 1. pp. 102-106.
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