Amino acid sequence of pheromone-inducible surface protein in Enterococcus faecalis, that is encoded on the conjugative plasmid pPD1

Jiro Nakayama, Hiromich Nagasawa, Akira Isogai, Don B. Clewell, Akinori Suzuki

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The major pheromone-inducible protein, PD78, believed to contribute to bacterial conjugation, was purified from Enterococcus (formerly Streptococcus) faecalis cells containing the plasmid pPD1. A cloned EcoRI-Bg/II 3.6-kbp fragment of the plasmid pAM351 (pPD1::Tn916) contained an open reading frame corresponding to 467 amino acid residues representing PD78. In a central region of the deduced protein, there is a repeated sequence of X-X-Pro that is repeated 15 times. This is analogous to the Gln-Gln-Pro repeat in the C-terminal region of TraD product encoded on the R 100 plasmid in Escherichia coli.

Original languageEnglish
Pages (from-to)81-84
Number of pages4
JournalFEBS Letters
Volume267
Issue number1
DOIs
Publication statusPublished - Jul 2 1990

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Enterococcus faecalis
Pheromones
Amino Acid Sequence
Membrane Proteins
Genetic Conjugation
Plasmids
Amino Acids
R Factors
Enterococcus
Open Reading Frames
Proteins
Escherichia coli

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Amino acid sequence of pheromone-inducible surface protein in Enterococcus faecalis, that is encoded on the conjugative plasmid pPD1. / Nakayama, Jiro; Nagasawa, Hiromich; Isogai, Akira; Clewell, Don B.; Suzuki, Akinori.

In: FEBS Letters, Vol. 267, No. 1, 02.07.1990, p. 81-84.

Research output: Contribution to journalArticle

Nakayama, Jiro ; Nagasawa, Hiromich ; Isogai, Akira ; Clewell, Don B. ; Suzuki, Akinori. / Amino acid sequence of pheromone-inducible surface protein in Enterococcus faecalis, that is encoded on the conjugative plasmid pPD1. In: FEBS Letters. 1990 ; Vol. 267, No. 1. pp. 81-84.
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