Aminoacylation of the N-terminal cysteine is essential for Lol-dependent release of lipoproteins from membranes but does not depend on lipoprotein sorting signals

Ayumu Fukuda, Shin Ichi Matsuyama, Takashi Hara, Jiro Nakayama, Hiromichi Nagasawa, Hajime Tokuda

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Lipoproteins are present in a wide variety of bacteria and are anchored to membranes through lipids attached to the N-terminal cysteine. The Lol system of Escherichia coli mediates the membrane-specific localization of lipoproteins. Aspartate at position 2 functions as a Lol avoidance signal and causes the retention of lipoproteins in the inner membrane, whereas lipoproteins having residues other than aspartate at position 2 are released from the inner membrane and localized to the outer membrane by the Lol system. Phospholipid:apolipoprotein transacylase, Lnt, catalyzes the last step of lipoprotein modification, converting apolipoprotein into mature lipoprotein. To reveal the importance of this aminoacylation for the Lol-dependent membrane localization, apolipoproteins were prepared by inhibiting lipoprotein maturation. Lnt was also purified and used to convert apolipoprotein into mature lipoprotein in vitro. The release of these lipoproteins was examined in proteoliposomes. We show here that the aminoacylation is essential for the Lol-dependent release of lipoproteins from membranes. Furthermore, lipoproteins with aspartate at position 2 were found to be aminoacylated both in vivo and in vitro, indicating that the lipoproteinsorting signal does not affect lipid modification.

Original languageEnglish
Pages (from-to)43512-43518
Number of pages7
JournalJournal of Biological Chemistry
Volume277
Issue number45
DOIs
Publication statusPublished - Nov 8 2002
Externally publishedYes

Fingerprint

Aminoacylation
Sorting
Lipoproteins
Cysteine
Membranes
Apolipoproteins
Aspartic Acid
Lipids
Membrane Lipids
Escherichia coli
Phospholipids
Bacteria

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Aminoacylation of the N-terminal cysteine is essential for Lol-dependent release of lipoproteins from membranes but does not depend on lipoprotein sorting signals. / Fukuda, Ayumu; Matsuyama, Shin Ichi; Hara, Takashi; Nakayama, Jiro; Nagasawa, Hiromichi; Tokuda, Hajime.

In: Journal of Biological Chemistry, Vol. 277, No. 45, 08.11.2002, p. 43512-43518.

Research output: Contribution to journalArticle

Fukuda, Ayumu ; Matsuyama, Shin Ichi ; Hara, Takashi ; Nakayama, Jiro ; Nagasawa, Hiromichi ; Tokuda, Hajime. / Aminoacylation of the N-terminal cysteine is essential for Lol-dependent release of lipoproteins from membranes but does not depend on lipoprotein sorting signals. In: Journal of Biological Chemistry. 2002 ; Vol. 277, No. 45. pp. 43512-43518.
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