Amounts of proteins altered by mutations in the dnaA gene of Escherichia coli

Akiko Ohba, Tohru Mizushima, Tsutomu Katayama, Kazuhisa Sekimizu

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We identified proteins whose amounts were altered in a temperature-sensitive dnaA46 mutant of Escherichia coli. Proteins whose amounts were increased in the mutant were serine hydroxymethyltransferase, β-ketoacyl [acyl carrier protein] synthase II, long-chain fatty acid transport protein, and UDP-glucose 4-epimerase, while the decreased ones were flagellin and D-ribose-binding protein. Transformation of the mutant with a plasmid containing the wild type dnaA gene complemented the phenotype. As pulse-labeling experiments revealed that the rates of synthesis of the proteins were altered in the mutant, DnaA protein may be involved in expression of these proteins.

Original languageEnglish
Pages (from-to)125-128
Number of pages4
JournalFEBS Letters
Volume404
Issue number2-3
DOIs
Publication statusPublished - Mar 10 1997

Fingerprint

Escherichia coli
Genes
Mutation
holo-(acyl-carrier-protein) synthase
Fatty Acid Transport Proteins
Proteins
UDPglucose 4-Epimerase
Glycine Hydroxymethyltransferase
Flagellin
Ribose
Mutant Proteins
Carrier Proteins
Plasmids
Labeling
Phenotype
Temperature
Experiments

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Amounts of proteins altered by mutations in the dnaA gene of Escherichia coli. / Ohba, Akiko; Mizushima, Tohru; Katayama, Tsutomu; Sekimizu, Kazuhisa.

In: FEBS Letters, Vol. 404, No. 2-3, 10.03.1997, p. 125-128.

Research output: Contribution to journalArticle

Ohba, Akiko ; Mizushima, Tohru ; Katayama, Tsutomu ; Sekimizu, Kazuhisa. / Amounts of proteins altered by mutations in the dnaA gene of Escherichia coli. In: FEBS Letters. 1997 ; Vol. 404, No. 2-3. pp. 125-128.
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