TY - JOUR
T1 - Amounts of proteins altered by mutations in the dnaA gene of Escherichia coli
AU - Ohba, Akiko
AU - Mizushima, Tohru
AU - Katayama, Tsutomu
AU - Sekimizu, Kazuhisa
N1 - Funding Information:
We are grateful to Drs. Y. Ishii and Y. Itoh (Kyushu University) for assistance with performing two-dimensional gel electrophoresis and protein sequencing, respectively, and M. Ohara for helpful comments. This work was supported in part by Grants in Aid for Scientific Research from the Ministry of Education, Science and Culture, Japan.
Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1997/3/10
Y1 - 1997/3/10
N2 - We identified proteins whose amounts were altered in a temperature-sensitive dnaA46 mutant of Escherichia coli. Proteins whose amounts were increased in the mutant were serine hydroxymethyltransferase, β-ketoacyl [acyl carrier protein] synthase II, long-chain fatty acid transport protein, and UDP-glucose 4-epimerase, while the decreased ones were flagellin and D-ribose-binding protein. Transformation of the mutant with a plasmid containing the wild type dnaA gene complemented the phenotype. As pulse-labeling experiments revealed that the rates of synthesis of the proteins were altered in the mutant, DnaA protein may be involved in expression of these proteins.
AB - We identified proteins whose amounts were altered in a temperature-sensitive dnaA46 mutant of Escherichia coli. Proteins whose amounts were increased in the mutant were serine hydroxymethyltransferase, β-ketoacyl [acyl carrier protein] synthase II, long-chain fatty acid transport protein, and UDP-glucose 4-epimerase, while the decreased ones were flagellin and D-ribose-binding protein. Transformation of the mutant with a plasmid containing the wild type dnaA gene complemented the phenotype. As pulse-labeling experiments revealed that the rates of synthesis of the proteins were altered in the mutant, DnaA protein may be involved in expression of these proteins.
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U2 - 10.1016/S0014-5793(97)00108-7
DO - 10.1016/S0014-5793(97)00108-7
M3 - Article
C2 - 9119048
AN - SCOPUS:0031048751
VL - 404
SP - 125
EP - 128
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 2-3
ER -